##################################
# NO CUT ENZYMES ( 38 enzymes) #
##################################
AatII AgeI AscI AvrII BlnI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BstBI ClaI CpoI
FseI MluI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PvuI RcaI RsrII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 8112 GGTACC G^GTACC
AclI 2060 AACGTT AA^CGTT
AflII 2030 CTTAAG C^TTAAG
Alw44I 6554 GTGCAC G^TGCAC
ApaLI 6554 GTGCAC G^TGCAC
AseI 392 ATTAAT AT^TAAT
AsnI 392 ATTAAT AT^TAAT
Asp718I 8112 GGTACC G^GTACC
AviII 8338 TGCGCA TGC^GCA
BfrI 2030 CTTAAG C^TTAAG
BsaBI 8629 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI 4908 CTCGTG CACGAG(-5/-1)
Bst1107I 3178 GTATAC GTA^TAC
BstZ17I 3178 GTATAC GTA^TAC
DrdI 7468 GACGTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1467 GATATC GAT^ATC
FspI 8338 TGCGCA TGC^GCA
KpnI 8112 GGTACC GGTAC^C
KspI 3715 CCGCGG CCGC^GG
MamI 8629 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5060 GCCGGC GCC^GGC
NgoMIV 5060 GCCGGC G^CCGGC
PshBI 392 ATTAAT AT^TAAT
Psp1406I 2060 AACGTT AA^CGTT
SacI 5229 GAGCTC GAGCT^C
SacII 3715 CCGCGG CCGC^GG
SalI 576 GTCGAC G^TCGAC
SapI 7173 GAAGAGC GCTCTTC(1/4)
SphI 5011 GCATGC GCATG^C
SspI 3948 AATATT AAT^ATT
StuI 8119 AGGCCT AGG^CCT
XbaI 6926 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 2952 ACACGT A^C[GA][CT]GT
7469 ACGTGT
Aor51HI 2016 AGCGCT AGC^GCT
6933 AGCGCT
BamHI 1703 GGATCC G^GATCC
6449 GGATCC
BbrPI 2529 CACGTG CAC^GTG
5213 CACGTG
BciVI 61 GGATAC GTATCC(6/5)
4269 GTATCC
BclI 203 TGATCA T^GATCA
2801 TGATCA
BglII 4208 AGATCT A^GATCT
4609 AGATCT
BsmBI 4468 CGTCTC CGTCTC(1/5)
4745 CGTCTC
BstAPI 7683 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7891 GCAGTAACTGC
Eco47III 2016 AGCGCT AGC^GCT
6933 AGCGCT
EcoRI 1489 GAATTC G^AATTC
2997 GAATTC
EcoT22I 316 ATGCAT ATGCA^T
3319 ATGCAT
FbaI 203 TGATCA T^GATCA
2801 TGATCA
MfeI 2872 CAATTG C^AATTG
3354 CAATTG
MunI 2872 CAATTG C^AATTG
3354 CAATTG
NsiI 316 ATGCAT ATGCA^T
3319 ATGCAT
PaeR7I 2448 CTCGAG C^TCGAG
4938 CTCGAG
PmaCI 2529 CACGTG CAC^GTG
5213 CACGTG
PmlI 2529 CACGTG CAC^GTG
5213 CACGTG
ScaI 3340 AGTACT AGT^ACT
5278 AGTACT
XhoI 2448 CTCGAG C^TCGAG
4938 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 167 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI