################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AclI AflIII AgeI Aor51HI AscI AseI AsnI Asp700I BclI BglII BsaBI BsiWI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AatII 5318 GACGTC GACGT^C Acc65I 2038 GGTACC G^GTACC AccI 5099 GTCTAC GT^[AC][GT]AC AccIII 1714 TCCGGA T^CCGGA AcsI 5081 AAATTT [GA]^AATT[CT] AflII 3715 CTTAAG C^TTAAG AhdI 3744 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 5081 AAATTT [GA]^AATT[CT] Asp718I 2038 GGTACC G^GTACC AspEI 3744 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 3181 GGATCC G^GATCC BfrI 3715 CTTAAG C^TTAAG BseAI 1714 TCCGGA T^CCGGA BsiEI 266 CGGCCG CG[GA][CT]^CG BsmI 4032 GAATGC GAATGC(1/-1) Bsp1407I 5476 TGTACA T^GTACA BspEI 1714 TCCGGA T^CCGGA BsrFI 4609 GCCGGC [GA]^CCGG[CT] BsrGI 5476 TGTACA T^GTACA Cfr10I 4609 GCCGGC [GA]^CCGG[CT] EagI 266 CGGCCG C^GGCCG Eam1105I 3744 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 266 CGGCCG C^GGCCG Eco52I 266 CGGCCG C^GGCCG HindIII 805 AAGCTT A^AGCTT KpnI 2038 GGTACC GGTAC^C MroI 1714 TCCGGA T^CCGGA NaeI 4609 GCCGGC GCC^GGC NgoMIV 4609 GCCGGC G^CCGGC PshAI 3379 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfiI 762 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 5476 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 118 GTGCAC G^TGCAC 5269 GTGCAC ApaLI 118 GTGCAC G^TGCAC 5269 GTGCAC BbrPI 2602 CACGTG CAC^GTG 3217 CACGTG BciVI 2813 GTATCC GTATCC(6/5) 2856 GTATCC BlpI 2526 GCTCAGC GC^T[ACGT]AGC 4395 GCTGAGC Bpu1102I 2526 GCTCAGC GC^T[ACGT]AGC 4395 GCTGAGC BsaAI 2602 CACGTG [CT]AC^GT[GA] 3217 CACGTG BsaWI 287 TCCGGT [AT]^CCGG[AT] 1714 TCCGGA BssHII 470 GCGCGC G^CGCGC 731 GCGCGC BstYI 1610 AGATCC [GA]^GATC[CT] 3181 GGATCC CelII 2526 GCTCAGC GC^T[ACGT]AGC 4395 GCTGAGC DraIII 3188 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 4841 CACCCCGTG DrdI 438 GACCGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3070 GACTGAAGGGTC MflI 1610 AGATCC [GA]^GATC[CT] 3181 GGATCC NcoI 3013 CCATGG C^CATGG 4522 CCATGG PflMI 3187 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4840 CCACCCCGTGG PmaCI 2602 CACGTG CAC^GTG 3217 CACGTG PmlI 2602 CACGTG CAC^GTG 3217 CACGTG SphI 1368 GCATGC GCATG^C 2679 GCATGC Van91I 3187 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4840 CCACCCCGTGG XcmI 3971 CCATCAGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4658 CCATCAAAGACATGG XhoII 1610 AGATCC [GA]^GATC[CT] 3181 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XmaCI XmaI