##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AclI        AflIII      AgeI        Aor51HI     AscI        
AseI        AsnI        Asp700I     BclI        BglII       
BsaBI       BsiWI       BspDI       BspHI       BspLU11I    
BsrDI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HpaI        MamI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII        5318  GACGTC               GACGT^C
Acc65I       2038  GGTACC               G^GTACC
AccI         5099  GTCTAC               GT^[AC][GT]AC
AccIII       1714  TCCGGA               T^CCGGA
AcsI         5081  AAATTT               [GA]^AATT[CT]
AflII        3715  CTTAAG               C^TTAAG
AhdI         3744  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         5081  AAATTT               [GA]^AATT[CT]
Asp718I      2038  GGTACC               G^GTACC
AspEI        3744  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        3181  GGATCC               G^GATCC
BfrI         3715  CTTAAG               C^TTAAG
BseAI        1714  TCCGGA               T^CCGGA
BsiEI         266  CGGCCG               CG[GA][CT]^CG
BsmI         4032  GAATGC               GAATGC(1/-1)
Bsp1407I     5476  TGTACA               T^GTACA
BspEI        1714  TCCGGA               T^CCGGA
BsrFI        4609  GCCGGC               [GA]^CCGG[CT]
BsrGI        5476  TGTACA               T^GTACA
Cfr10I       4609  GCCGGC               [GA]^CCGG[CT]
EagI          266  CGGCCG               C^GGCCG
Eam1105I     3744  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         266  CGGCCG               C^GGCCG
Eco52I        266  CGGCCG               C^GGCCG
HindIII       805  AAGCTT               A^AGCTT
KpnI         2038  GGTACC               GGTAC^C
MroI         1714  TCCGGA               T^CCGGA
NaeI         4609  GCCGGC               GCC^GGC
NgoMIV       4609  GCCGGC               G^CCGGC
PshAI        3379  GACAAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI          762  GGCCTTCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        5476  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Alw44I        118  GTGCAC               G^TGCAC
             5269  GTGCAC              
ApaLI         118  GTGCAC               G^TGCAC
             5269  GTGCAC              
BbrPI        2602  CACGTG               CAC^GTG
             3217  CACGTG              
BciVI        2813  GTATCC               GTATCC(6/5)
             2856  GTATCC              
BlpI         2526  GCTCAGC              GC^T[ACGT]AGC
             4395  GCTGAGC             
Bpu1102I     2526  GCTCAGC              GC^T[ACGT]AGC
             4395  GCTGAGC             
BsaAI        2602  CACGTG               [CT]AC^GT[GA]
             3217  CACGTG              
BsaWI         287  TCCGGT               [AT]^CCGG[AT]
             1714  TCCGGA              
BssHII        470  GCGCGC               G^CGCGC
              731  GCGCGC              
BstYI        1610  AGATCC               [GA]^GATC[CT]
             3181  GGATCC              
CelII        2526  GCTCAGC              GC^T[ACGT]AGC
             4395  GCTGAGC             
DraIII       3188  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4841  CACCCCGTG           
DrdI          438  GACCGTGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3070  GACTGAAGGGTC        
MflI         1610  AGATCC               [GA]^GATC[CT]
             3181  GGATCC              
NcoI         3013  CCATGG               C^CATGG
             4522  CCATGG              
PflMI        3187  CCACAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4840  CCACCCCGTGG         
PmaCI        2602  CACGTG               CAC^GTG
             3217  CACGTG              
PmlI         2602  CACGTG               CAC^GTG
             3217  CACGTG              
SphI         1368  GCATGC               GCATG^C
             2679  GCATGC              
Van91I       3187  CCACAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4840  CCACCCCGTGG         
XcmI         3971  CCATCAGGCAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4658  CCATCAAAGACATGG     
XhoII        1610  AGATCC               [GA]^GATC[CT]
             3181  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcgI        BcnI        BfaI        
BglI        BlnI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SacII       
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
XmaCI       XmaI