##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AflII AscI AseI AsnI
AviII BbeI BciVI BclI BfrI
BsaBI BsiWI Bsp1407I BspDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI MamI MfeI MluI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfoI SfuI SgrAI SnaBI SphI
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 7604 GGTACC G^GTACC
AccIII 6145 TCCGGA T^CCGGA
AcsI 5338 AAATTC [GA]^AATT[CT]
Aor51HI 6305 AGCGCT AGC^GCT
ApoI 5338 AAATTC [GA]^AATT[CT]
Asp718I 7604 GGTACC G^GTACC
BglII 1627 AGATCT A^GATCT
BseAI 6145 TCCGGA T^CCGGA
BsmI 7461 GAATGC GAATGC(1/-1)
BspEI 6145 TCCGGA T^CCGGA
BspHI 5706 TCATGA T^CATGA
BspLU11I 5650 ACATGT A^CATGT
BssHII 586 GCGCGC G^CGCGC
Eco47III 6305 AGCGCT AGC^GCT
KpnI 7604 GGTACC GGTAC^C
KspI 642 CCGCGG CCGC^GG
MroI 6145 TCCGGA T^CCGGA
NheI 3924 GCTAGC G^CTAGC
PaeR7I 2988 CTCGAG C^TCGAG
RcaI 5706 TCATGA T^CATGA
SacII 642 CCGCGG CCGC^GG
SspI 4923 AATATT AAT^ATT
XhoI 2988 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AgeI 2966 ACCGGT A^CCGGT
3129 ACCGGT
AhdI 417 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1734 GACCCTGTGTC
AspEI 417 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1734 GACCCTGTGTC
BamHI 11 GGATCC G^GATCC
7391 GGATCC
BcgI 1294 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4085 GCAGCGGCCTCG
BsrBI 2772 CCGCTC CCGCTC(-3/-3)
2815 GAGCGG
DraI 7832 TTTAAA TTT^AAA
7995 TTTAAA
Eam1105I 417 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1734 GACCCTGTGTC
NaeI 365 GCCGGC GCC^GGC
2851 GCCGGC
NcoI 2140 CCATGG C^CATGG
3404 CCATGG
NgoMIV 365 GCCGGC G^CCGGC
2851 GCCGGC
PinAI 2966 ACCGGT A^CCGGT
3129 ACCGGT
SapI 4056 GAAGAGC GCTCTTC(1/4)
2180 GCTCTTC
SexAI 877 ACCTGGT A^CC[AT]GGT
1039 ACCTGGT
SpeI 3564 ACTAGT A^CTAGT
4934 ACTAGT
XbaI 792 TCTAGA T^CTAGA
7468 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI Asp700I
AspHI AspI AvaI AvaII AvrII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfiI SmaI SmlI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI