##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BciVI BclI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NheI NotI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI ScaI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2310 GTCTAC GT^[AC][GT]AC
AflIII 271 ACGTGT A^C[GA][CT]GT
Alw44I 447 GTGCAC G^TGCAC
ApaLI 447 GTGCAC G^TGCAC
Asp700I 200 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1440 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1234 TGCGCA TGC^GCA
BamHI 227 GGATCC G^GATCC
BbeI 22 GGCGCC GGCGC^C
BfaI 1740 CTAG C^TAG
BlpI 2025 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2025 GCTCAGC GC^T[ACGT]AGC
BsiEI 502 CGGTCG CG[GA][CT]^CG
BspDI 2433 ATCGAT AT^CGAT
CelII 2025 GCTCAGC GC^T[ACGT]AGC
ClaI 2433 ATCGAT AT^CGAT
FspI 1234 TGCGCA TGC^GCA
HindIII 2373 AAGCTT A^AGCTT
KasI 22 GGCGCC G^GCGCC
MaeI 1740 CTAG C^TAG
NarI 22 GGCGCC GG^CGCC
NcoI 3454 CCATGG C^CATGG
NdeI 1243 CATATG CA^TATG
NruI 962 TCGCGA TCG^CGA
NspI 3034 GCATGT [GA]CATG^[CT]
PflFI 1440 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3454 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI 2006 GGCCCCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 22 GGCGCC GGC^GCC
Tth111I 1440 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3454 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 200 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AatII 1248 GACGTC GACGT^C
1827 GACGTC
BbrPI 270 CACGTG CAC^GTG
3373 CACGTG
BsrFI 2042 GCCGGC [GA]^CCGG[CT]
2455 GCCGGC
Cfr10I 2042 GCCGGC [GA]^CCGG[CT]
2455 GCCGGC
DraIII 441 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
450 CACAGCGTG
NaeI 2042 GCCGGC GCC^GGC
2455 GCCGGC
NgoMIV 2042 GCCGGC G^CCGGC
2455 GCCGGC
PaeR7I 116 CTCGAG C^TCGAG
2925 CTCGAG
PmaCI 270 CACGTG CAC^GTG
3373 CACGTG
PmlI 270 CACGTG CAC^GTG
3373 CACGTG
SapI 1152 GAAGAGC GCTCTTC(1/4)
2813 GAAGAGC
SexAI 622 ACCTGGT A^CC[AT]GGT
3072 ACCAGGT
XhoI 116 CTCGAG C^TCGAG
2925 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI XhoII