##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
BcgI        BclI        BglII       BsaBI       BsiWI       
BsmBI       Bsp1407I    BspHI       BsrGI       BssHII      
Bst1107I    BstBI       BstUI       BstZ17I     CpoI        
Eam1105I    Eco47III    EcoRI       EcoRV       FbaI        
HgaI        HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NotI        
NruI        NspV        PacI        PinAI       PmeI        
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         4495  GTCTAC               GT^[AC][GT]AC
AccIII        268  TCCGGA               T^CCGGA
AflII          99  CTTAAG               C^TTAAG
Alw44I       1296  GTGCAC               G^TGCAC
ApaLI        1296  GTGCAC               G^TGCAC
AseI         5297  ATTAAT               AT^TAAT
AsnI         5297  ATTAAT               AT^TAAT
Asp700I      4009  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        3184  TGCGCA               TGC^GCA
AvrII        3941  CCTAGG               C^CTAGG
BamHI        1128  GGATCC               G^GATCC
BbrPI        4320  CACGTG               CAC^GTG
BfrI           99  CTTAAG               C^TTAAG
BlnI         3941  CCTAGG               C^CTAGG
BlpI         3729  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3729  GCTCAGC              GC^T[ACGT]AGC
BsaAI        4320  CACGTG               [CT]AC^GT[GA]
BsaWI         268  TCCGGA               [AT]^CCGG[AT]
BseAI         268  TCCGGA               T^CCGGA
BsiEI        5648  CGGCCG               CG[GA][CT]^CG
BspDI        4227  ATCGAT               AT^CGAT
BspEI         268  TCCGGA               T^CCGGA
BspLU11I      565  ACATGT               A^CATGT
BstEII       5255  GGTTACC              G^GT[ACGT]ACC
BstPI        5255  GGTTACC              G^GT[ACGT]ACC
CelII        3729  GCTCAGC              GC^T[ACGT]AGC
ClaI         4227  ATCGAT               AT^CGAT
EagI         5648  CGGCCG               C^GGCCG
EclXI        5648  CGGCCG               C^GGCCG
Eco52I       5648  CGGCCG               C^GGCCG
EcoO65I      5255  GGTTACC              G^GT[ACGT]ACC
FseI          711  GGCCGGCC             GGCCGG^CC
FspI         3184  TGCGCA               TGC^GCA
MroI          268  TCCGGA               T^CCGGA
NaeI          712  GCCGGC               GCC^GGC
NdeI         5287  CATATG               CA^TATG
NgoMIV        712  GCCGGC               G^CCGGC
NheI         1196  GCTAGC               G^CTAGC
PmaCI        4320  CACGTG               CAC^GTG
PmlI         4320  CACGTG               CAC^GTG
PshAI        5206  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        5297  ATTAAT               AT^TAAT
SspI         3873  AATATT               AAT^ATT
XmnI         4009  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AflIII        433  ACGTGT               A^C[GA][CT]GT
              565  ACATGT              
BbsI         2963  GAAGAC               GAAGAC(2/6)
             2015  GTCTTC              
BciVI         807  GGATAC               GTATCC(6/5)
             5585  GGATAC              
BglI         1078  GCCAACAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4972  GCCTTGAGGGC         
BpuAI        2963  GAAGAC               GAAGAC(2/6)
             2015  GTCTTC              
BsrBI         126  CCGCTC               CCGCTC(-3/-3)
             1754  CCGCTC              
BsrDI         465  CATTGC               GCAATG(2/0)
             5245  CATTGC              
BssSI        5710  CACGAG               CACGAG(-5/-1)
             5537  CTCGTG              
DraI         5111  TTTAAA               TTT^AAA
             5370  TTTAAA              
DraIII       2123  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3634  CACAAAGTG           
DrdI         2966  GACCAGCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4158  GACAAAGTGGTC        
EcoT22I       375  ATGCAT               ATGCA^T
             2816  ATGCAT              
HincII       3957  GTTGAC               GT[CT]^[GA]AC
             4167  GTCAAC              
HindII       3957  GTTGAC               GT[CT]^[GA]AC
             4167  GTCAAC              
HindIII      4712  AAGCTT               A^AGCTT
             5015  AAGCTT              
NcoI         2248  CCATGG               C^CATGG
             2500  CCATGG              
NsiI          375  ATGCAT               ATGCA^T
             2816  ATGCAT              
PaeR7I       2862  CTCGAG               C^TCGAG
             3620  CTCGAG              
ScaI         4787  AGTACT               AGT^ACT
             4856  AGTACT              
SexAI        2124  ACCTGGT              A^CC[AT]GGT
             5105  ACCAGGT             
XbaI         2621  TCTAGA               T^CTAGA
             5322  TCTAGA              
XhoI         2862  CTCGAG               C^TCGAG
             3620  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
AspHI       AspI        AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI