##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AatII       Acc65I      AclI        AhdI        Aor51HI     
AscI        Asp718I     AspEI       AviII       BclI        
BsaBI       BsiWI       BssHII      BstBI       BstXI       
CpoI        Eam1105I    Eco47III    EcoRV       EcoT22I     
FbaI        FseI        FspI        KpnI        KspI        
MamI        MfeI        MunI        NdeI        NotI        
NruI        NsiI        NspV        PacI        Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII       4247  TCCGGA               T^CCGGA
AflII        6260  CTTAAG               C^TTAAG
AgeI         2926  ACCGGT               A^CCGGT
Alw44I       5105  GTGCAC               G^TGCAC
ApaI          538  GGGCCC               GGGCC^C
ApaLI        5105  GTGCAC               G^TGCAC
AspI         1934  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        5076  GGATCC               G^GATCC
BbeI          152  GGCGCC               GGCGC^C
BcgI          100  CGAGCGGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3551  GTATCC               GTATCC(6/5)
BfrI         6260  CTTAAG               C^TTAAG
BglI          131  GCCGGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI        4247  TCCGGA               T^CCGGA
BsiEI          54  CGGCCG               CG[GA][CT]^CG
BsmI         3019  GAATGC               GAATGC(1/-1)
BspDI        2707  ATCGAT               AT^CGAT
BspEI        4247  TCCGGA               T^CCGGA
BssSI         345  CTCGTG               CACGAG(-5/-1)
BstAPI        850  GCATCGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI         2707  ATCGAT               AT^CGAT
DrdI         1920  GACAAGCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           54  CGGCCG               C^GGCCG
EclXI          54  CGGCCG               C^GGCCG
Eco52I         54  CGGCCG               C^GGCCG
HaeII         152  GGCGCC               [GA]GCGC^[CT]
HpaI         4461  GTTAAC               GTT^AAC
KasI          152  GGCGCC               G^GCGCC
MluI         6391  ACGCGT               A^CGCGT
MroI         4247  TCCGGA               T^CCGGA
NaeI           52  GCCGGC               GCC^GGC
NarI          152  GGCGCC               GG^CGCC
NcoI         1496  CCATGG               C^CATGG
NgoMIV         52  GCCGGC               G^CCGGC
NheI         4749  GCTAGC               G^CTAGC
PaeR7I        592  CTCGAG               C^TCGAG
PflFI        1934  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1696  CCATATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2926  ACCGGT               A^CCGGT
PmeI         4641  GTTTAAAC             GTTT^AAAC
ScaI         6264  AGTACT               AGT^ACT
SfoI          152  GGCGCC               GGC^GCC
SmaI          111  CCCGGG               CCC^GGG
Tth111I      1934  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1696  CCATATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         5987  TCTAGA               T^CTAGA
XhoI          592  CTCGAG               C^TCGAG
XmaCI         111  CCCGGG               C^CCGGG
XmaI          111  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI           46  GGCGTC               G[GA]^CG[CT]C
              152  GGCGCC              
Asp700I      1664  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2999  GAAACATTTC          
AvrII        4294  CCTAGG               C^CTAGG
             5587  CCTAGG              
BbrPI        2536  CACGTG               CAC^GTG
             5052  CACGTG              
BbvCI        1801  CCTCAGC              CCTCAGC(-5/-2)
             4114  CCTCAGC             
BglII        3324  AGATCT               A^GATCT
             4771  AGATCT              
BlnI         4294  CCTAGG               C^CTAGG
             5587  CCTAGG              
BsaHI          46  GGCGTC               G[GA]^CG[CT]C
              152  GGCGCC              
BsmBI        6455  CGTCTC               CGTCTC(1/5)
             3973  GAGACG              
BspMI        4923  ACCTGC               ACCTGC(4/8)
             2370  GCAGGT              
BsrBI         101  GAGCGG               CCGCTC(-3/-3)
              147  GAGCGG              
BsrFI          52  GCCGGC               [GA]^CCGG[CT]
             2926  ACCGGT              
BstEII       4741  GGTAACC              G^GT[ACGT]ACC
             5470  GGTTACC             
BstPI        4741  GGTAACC              G^GT[ACGT]ACC
             5470  GGTTACC             
Cfr10I         52  GCCGGC               [GA]^CCGG[CT]
             2926  ACCGGT              
DsaI         1496  CCATGG               C^C[GA][CT]GG
             3410  CCACGG              
EcoNI        2131  CCTGGAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4924  CCTGCCCCAGG         
EcoO65I      4741  GGTAACC              G^GT[ACGT]ACC
             5470  GGTTACC             
Hin1I          46  GGCGTC               G[GA]^CG[CT]C
              152  GGCGCC              
HindIII       274  AAGCTT               A^AGCTT
             5790  AAGCTT              
PmaCI        2536  CACGTG               CAC^GTG
             5052  CACGTG              
PmlI         2536  CACGTG               CAC^GTG
             5052  CACGTG              
SacI           14  GAGCTC               GAGCT^C
             6096  GAGCTC              
StuI         2401  AGGCCT               AGG^CCT
             6360  AGGCCT              
XmnI         1664  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2999  GAAACATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        AseI        AsnI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspHI       BspLU11I    BsrDI       
BsrGI       BsrI        BssKI       Bst1107I    BstF5I      
BstNI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DraIII      EaeI        
EarI        Eco81I      EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PshAI       
PshBI       PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII