################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AatII Acc65I AclI AhdI Aor51HI AscI Asp718I AspEI AviII BclI BsaBI BsiWI BssHII BstBI BstXI CpoI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI FspI KpnI KspI MamI MfeI MunI NdeI NotI NruI NsiI NspV PacI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 4247 TCCGGA T^CCGGA AflII 6260 CTTAAG C^TTAAG AgeI 2926 ACCGGT A^CCGGT Alw44I 5105 GTGCAC G^TGCAC ApaI 538 GGGCCC GGGCC^C ApaLI 5105 GTGCAC G^TGCAC AspI 1934 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 5076 GGATCC G^GATCC BbeI 152 GGCGCC GGCGC^C BcgI 100 CGAGCGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3551 GTATCC GTATCC(6/5) BfrI 6260 CTTAAG C^TTAAG BglI 131 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 4247 TCCGGA T^CCGGA BsiEI 54 CGGCCG CG[GA][CT]^CG BsmI 3019 GAATGC GAATGC(1/-1) BspDI 2707 ATCGAT AT^CGAT BspEI 4247 TCCGGA T^CCGGA BssSI 345 CTCGTG CACGAG(-5/-1) BstAPI 850 GCATCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 2707 ATCGAT AT^CGAT DrdI 1920 GACAAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 54 CGGCCG C^GGCCG EclXI 54 CGGCCG C^GGCCG Eco52I 54 CGGCCG C^GGCCG HaeII 152 GGCGCC [GA]GCGC^[CT] HpaI 4461 GTTAAC GTT^AAC KasI 152 GGCGCC G^GCGCC MluI 6391 ACGCGT A^CGCGT MroI 4247 TCCGGA T^CCGGA NaeI 52 GCCGGC GCC^GGC NarI 152 GGCGCC GG^CGCC NcoI 1496 CCATGG C^CATGG NgoMIV 52 GCCGGC G^CCGGC NheI 4749 GCTAGC G^CTAGC PaeR7I 592 CTCGAG C^TCGAG PflFI 1934 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1696 CCATATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2926 ACCGGT A^CCGGT PmeI 4641 GTTTAAAC GTTT^AAAC ScaI 6264 AGTACT AGT^ACT SfoI 152 GGCGCC GGC^GCC SmaI 111 CCCGGG CCC^GGG Tth111I 1934 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1696 CCATATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 5987 TCTAGA T^CTAGA XhoI 592 CTCGAG C^TCGAG XmaCI 111 CCCGGG C^CCGGG XmaI 111 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 46 GGCGTC G[GA]^CG[CT]C 152 GGCGCC Asp700I 1664 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2999 GAAACATTTC AvrII 4294 CCTAGG C^CTAGG 5587 CCTAGG BbrPI 2536 CACGTG CAC^GTG 5052 CACGTG BbvCI 1801 CCTCAGC CCTCAGC(-5/-2) 4114 CCTCAGC BglII 3324 AGATCT A^GATCT 4771 AGATCT BlnI 4294 CCTAGG C^CTAGG 5587 CCTAGG BsaHI 46 GGCGTC G[GA]^CG[CT]C 152 GGCGCC BsmBI 6455 CGTCTC CGTCTC(1/5) 3973 GAGACG BspMI 4923 ACCTGC ACCTGC(4/8) 2370 GCAGGT BsrBI 101 GAGCGG CCGCTC(-3/-3) 147 GAGCGG BsrFI 52 GCCGGC [GA]^CCGG[CT] 2926 ACCGGT BstEII 4741 GGTAACC G^GT[ACGT]ACC 5470 GGTTACC BstPI 4741 GGTAACC G^GT[ACGT]ACC 5470 GGTTACC Cfr10I 52 GCCGGC [GA]^CCGG[CT] 2926 ACCGGT DsaI 1496 CCATGG C^C[GA][CT]GG 3410 CCACGG EcoNI 2131 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4924 CCTGCCCCAGG EcoO65I 4741 GGTAACC G^GT[ACGT]ACC 5470 GGTTACC Hin1I 46 GGCGTC G[GA]^CG[CT]C 152 GGCGCC HindIII 274 AAGCTT A^AGCTT 5790 AAGCTT PmaCI 2536 CACGTG CAC^GTG 5052 CACGTG PmlI 2536 CACGTG CAC^GTG 5052 CACGTG SacI 14 GAGCTC GAGCT^C 6096 GAGCTC StuI 2401 AGGCCT AGG^CCT 6360 AGGCCT XmnI 1664 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2999 GAAACATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII