################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AclI AhdI Aor51HI AscI Asp718I AspEI AspI BbrPI BciVI BclI BsaAI BsaBI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FbaI FseI HpaI KpnI MamI MluI NdeI NheI NotI NruI NsiI PacI PflFI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SnaBI SpeI SphI SspBI Tth111I ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 6281 GTATAC GT^[AC][GT]AC AflII 193 CTTAAG C^TTAAG AgeI 1640 ACCGGT A^CCGGT AseI 5014 ATTAAT AT^TAAT AsnI 5014 ATTAAT AT^TAAT AviII 381 TGCGCA TGC^GCA AvrII 243 CCTAGG C^CTAGG BfrI 193 CTTAAG C^TTAAG BlnI 243 CCTAGG C^CTAGG Bst1107I 6281 GTATAC GTA^TAC BstBI 1900 TTCGAA TT^CGAA BstEII 3338 GGTCACC G^GT[ACGT]ACC BstPI 3338 GGTCACC G^GT[ACGT]ACC BstZ17I 6281 GTATAC GTA^TAC DrdI 3947 GACCCAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3338 GGTCACC G^GT[ACGT]ACC EcoRI 3478 GAATTC G^AATTC EcoRV 5425 GATATC GAT^ATC FspI 381 TGCGCA TGC^GCA HincII 2911 GTCAAC GT[CT]^[GA]AC HindII 2911 GTCAAC GT[CT]^[GA]AC HindIII 5360 AAGCTT A^AGCTT MfeI 5710 CAATTG C^AATTG MunI 5710 CAATTG C^AATTG NcoI 1368 CCATGG C^CATGG NspV 1900 TTCGAA TT^CGAA PinAI 1640 ACCGGT A^CCGGT PshBI 5014 ATTAAT AT^TAAT SbfI 2407 CCTGCAGG CCTGCA^GG SfuI 1900 TTCGAA TT^CGAA SgrAI 1639 CACCGGTG C[GA]^CCGG[CT]G Sse8387I 2407 CCTGCAGG CCTGCA^GG SspI 1450 AATATT AAT^ATT SwaI 2325 ATTTAAAT ATTT^AAAT XbaI 3394 TCTAGA T^CTAGA XcmI 3950 CCAAAGGTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccIII 1852 TCCGGA T^CCGGA 2353 TCCGGA AcyI 1054 GGCGCC G[GA]^CG[CT]C 2273 GGCGCC AflIII 4949 ACACGT A^C[GA][CT]GT 5332 ACGTGT Alw44I 1188 GTGCAC G^TGCAC 5416 GTGCAC AocI 1066 CCTCAGG CC^T[ACGT]AGG 3541 CCTGAGG ApaLI 1188 GTGCAC G^TGCAC 5416 GTGCAC BamHI 1849 GGATCC G^GATCC 2692 GGATCC BbeI 1054 GGCGCC GGCGC^C 2273 GGCGCC BglI 112 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2780 GCCAGCCTGGC BsaHI 1054 GGCGCC G[GA]^CG[CT]C 2273 GGCGCC BseAI 1852 TCCGGA T^CCGGA 2353 TCCGGA BspEI 1852 TCCGGA T^CCGGA 2353 TCCGGA BspHI 531 TCATGA T^CATGA 1227 TCATGA BstAPI 2049 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2758 GCAGGGCCTGC Bsu36I 1066 CCTCAGG CC^T[ACGT]AGG 3541 CCTGAGG DraI 2326 TTTAAA TTT^AAA 5823 TTTAAA Eco81I 1066 CCTCAGG CC^T[ACGT]AGG 3541 CCTGAGG Hin1I 1054 GGCGCC G[GA]^CG[CT]C 2273 GGCGCC KasI 1054 GGCGCC G^GCGCC 2273 GGCGCC MroI 1852 TCCGGA T^CCGGA 2353 TCCGGA MslI 2324 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3958 CACATGGATG NaeI 281 GCCGGC GCC^GGC 356 GCCGGC NarI 1054 GGCGCC GG^CGCC 2273 GGCGCC NgoMIV 281 GCCGGC G^CCGGC 356 GCCGGC PaeR7I 2588 CTCGAG C^TCGAG 3431 CTCGAG RcaI 531 TCATGA T^CATGA 1227 TCATGA SfoI 1054 GGCGCC GGC^GCC 2273 GGCGCC SmaI 457 CCCGGG CCC^GGG 1745 CCCGGG XhoI 2588 CTCGAG C^TCGAG 3431 CTCGAG XmaCI 457 CCCGGG C^CCGGG 1745 CCCGGG XmaI 457 CCCGGG C^CCGGG 1745 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI