##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       Acc65I      AclI        AhdI        Aor51HI     
AscI        Asp718I     AspEI       AspI        BbrPI       
BciVI       BclI        BsaAI       BsaBI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      BssSI       ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoT22I     
FbaI        FseI        HpaI        KpnI        MamI        
MluI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PflFI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SalI        ScaI        SexAI       SfiI        SnaBI       
SpeI        SphI        SspBI       Tth111I     


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         6281  GTATAC               GT^[AC][GT]AC
AflII         193  CTTAAG               C^TTAAG
AgeI         1640  ACCGGT               A^CCGGT
AseI         5014  ATTAAT               AT^TAAT
AsnI         5014  ATTAAT               AT^TAAT
AviII         381  TGCGCA               TGC^GCA
AvrII         243  CCTAGG               C^CTAGG
BfrI          193  CTTAAG               C^TTAAG
BlnI          243  CCTAGG               C^CTAGG
Bst1107I     6281  GTATAC               GTA^TAC
BstBI        1900  TTCGAA               TT^CGAA
BstEII       3338  GGTCACC              G^GT[ACGT]ACC
BstPI        3338  GGTCACC              G^GT[ACGT]ACC
BstZ17I      6281  GTATAC               GTA^TAC
DrdI         3947  GACCCAAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3338  GGTCACC              G^GT[ACGT]ACC
EcoRI        3478  GAATTC               G^AATTC
EcoRV        5425  GATATC               GAT^ATC
FspI          381  TGCGCA               TGC^GCA
HincII       2911  GTCAAC               GT[CT]^[GA]AC
HindII       2911  GTCAAC               GT[CT]^[GA]AC
HindIII      5360  AAGCTT               A^AGCTT
MfeI         5710  CAATTG               C^AATTG
MunI         5710  CAATTG               C^AATTG
NcoI         1368  CCATGG               C^CATGG
NspV         1900  TTCGAA               TT^CGAA
PinAI        1640  ACCGGT               A^CCGGT
PshBI        5014  ATTAAT               AT^TAAT
SbfI         2407  CCTGCAGG             CCTGCA^GG
SfuI         1900  TTCGAA               TT^CGAA
SgrAI        1639  CACCGGTG             C[GA]^CCGG[CT]G
Sse8387I     2407  CCTGCAGG             CCTGCA^GG
SspI         1450  AATATT               AAT^ATT
SwaI         2325  ATTTAAAT             ATTT^AAAT
XbaI         3394  TCTAGA               T^CTAGA
XcmI         3950  CCAAAGGTCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1852  TCCGGA               T^CCGGA
             2353  TCCGGA              
AcyI         1054  GGCGCC               G[GA]^CG[CT]C
             2273  GGCGCC              
AflIII       4949  ACACGT               A^C[GA][CT]GT
             5332  ACGTGT              
Alw44I       1188  GTGCAC               G^TGCAC
             5416  GTGCAC              
AocI         1066  CCTCAGG              CC^T[ACGT]AGG
             3541  CCTGAGG             
ApaLI        1188  GTGCAC               G^TGCAC
             5416  GTGCAC              
BamHI        1849  GGATCC               G^GATCC
             2692  GGATCC              
BbeI         1054  GGCGCC               GGCGC^C
             2273  GGCGCC              
BglI          112  GCCCCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2780  GCCAGCCTGGC         
BsaHI        1054  GGCGCC               G[GA]^CG[CT]C
             2273  GGCGCC              
BseAI        1852  TCCGGA               T^CCGGA
             2353  TCCGGA              
BspEI        1852  TCCGGA               T^CCGGA
             2353  TCCGGA              
BspHI         531  TCATGA               T^CATGA
             1227  TCATGA              
BstAPI       2049  GCACGCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2758  GCAGGGCCTGC         
Bsu36I       1066  CCTCAGG              CC^T[ACGT]AGG
             3541  CCTGAGG             
DraI         2326  TTTAAA               TTT^AAA
             5823  TTTAAA              
Eco81I       1066  CCTCAGG              CC^T[ACGT]AGG
             3541  CCTGAGG             
Hin1I        1054  GGCGCC               G[GA]^CG[CT]C
             2273  GGCGCC              
KasI         1054  GGCGCC               G^GCGCC
             2273  GGCGCC              
MroI         1852  TCCGGA               T^CCGGA
             2353  TCCGGA              
MslI         2324  CATTTAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3958  CACATGGATG          
NaeI          281  GCCGGC               GCC^GGC
              356  GCCGGC              
NarI         1054  GGCGCC               GG^CGCC
             2273  GGCGCC              
NgoMIV        281  GCCGGC               G^CCGGC
              356  GCCGGC              
PaeR7I       2588  CTCGAG               C^TCGAG
             3431  CTCGAG              
RcaI          531  TCATGA               T^CATGA
             1227  TCATGA              
SfoI         1054  GGCGCC               GGC^GCC
             2273  GGCGCC              
SmaI          457  CCCGGG               CCC^GGG
             1745  CCCGGG              
XhoI         2588  CTCGAG               C^TCGAG
             3431  CTCGAG              
XmaCI         457  CCCGGG               C^CCGGG
             1745  CCCGGG              
XmaI          457  CCCGGG               C^CCGGG
             1745  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcgI        
BcnI        BfaI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SacII       SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI