##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AclI AfaI AflII AflIII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I EcoO65I EcoRV EcoT22I FbaI
HindIII HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MslI MunI NarI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 166 GTAGAC GT^[AC][GT]AC
AccIII 748 TCCGGA T^CCGGA
AcsI 835 GAATTC [GA]^AATT[CT]
AocI 1207 CCTGAGG CC^T[ACGT]AGG
ApoI 835 GAATTC [GA]^AATT[CT]
AviII 542 TGCGCA TGC^GCA
BanI 827 GGCACC G^G[CT][GA]CC
BcgI 77 GCATTTATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 613 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 140 GAGACC GGTCTC(1/5)
BseAI 748 TCCGGA T^CCGGA
BsmAI 140 GAGAC GTCTC(1/5)
BspEI 748 TCCGGA T^CCGGA
BspMI 494 ACCTGC ACCTGC(4/8)
BsrDI 108 CATTGC GCAATG(2/0)
BsrI 1024 ACTGG ACTGG(1/-1)
Bsu36I 1207 CCTGAGG CC^T[ACGT]AGG
DraII 68 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 245 GACCTGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1091 CCATGG C^C[GA][CT]GG
Eco81I 1207 CCTGAGG CC^T[ACGT]AGG
EcoNI 201 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 68 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 835 GAATTC G^AATTC
FseI 1212 GGCCGGCC GGCCGG^CC
FspI 542 TGCGCA TGC^GCA
HgaI 565 GACGC GACGC(5/10)
HincII 772 GTCAAC GT[CT]^[GA]AC
HindII 772 GTCAAC GT[CT]^[GA]AC
HphI 352 TCACC GGTGA(8/7)
MroI 748 TCCGGA T^CCGGA
MseI 158 TTAA T^TAA
NcoI 1091 CCATGG C^CATGG
NotI 1241 GCGGCCGC GC^GGCCGC
PpuMI 68 AGGACCT [GA]G^G[AT]CC[CT]
PstI 571 CTGCAG CTGCA^G
SfcI 571 CTGCAG C^T[GA][CT]AG
Tru9I 158 TTAA T^TAA
Tsp509I 836 AATT ^AATT
TspRI 1023 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 1230 CGCG CG^CG
1240 CGCG
AlwNI 427 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
874 CAGCAGCTG
Aor51HI 671 AGCGCT AGC^GCT
887 AGCGCT
BalI 194 TGGCCA TGG^CCA
803 TGGCCA
BamHI 128 GGATCC G^GATCC
1203 GGATCC
BssSI 796 CACGAG CACGAG(-5/-1)
958 CACGAG
BstUI 1230 CGCG CG^CG
1240 CGCG
BstYI 128 GGATCC [GA]^GATC[CT]
1203 GGATCC
Eco47III 671 AGCGCT AGC^GCT
887 AGCGCT
EcoT14I 1091 CCATGG C^C[AT][AT]GG
1151 CCAAGG
HinfI 252 GAGTC G^A[ACGT]TC
601 GACTC
MflI 128 GGATCC [GA]^GATC[CT]
1203 GGATCC
MluNI 194 TGGCCA TGG^CCA
803 TGGCCA
MscI 194 TGGCCA TGG^CCA
803 TGGCCA
MvnI 1230 CGCG CG^CG
1240 CGCG
PflMI 426 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
795 CCACGAGATGG
PleI 601 GACTC GAGTC(4/5)
252 GAGTC
StuI 411 AGGCCT AGG^CCT
899 AGGCCT
StyI 1091 CCATGG C^C[AT][AT]GG
1151 CCAAGG
Van91I 426 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
795 CCACGAGATGG
XhoII 128 GGATCC [GA]^GATC[CT]
1203 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AatII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EagI EarI EclXI Eco52I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SmaI TseI
XmaCI XmaI