##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AflIII      AhdI        AocI        Aor51HI     AscI        
Asp718I     AspEI       AvrII       BamHI       BciVI       
BclI        BfrI        BglII       BlnI        BsaBI       
BseAI       BsiWI       BsmI        BspDI       BspEI       
BspLU11I    Bst1107I    BstBI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        Eam1105I    
Eco47III    Eco81I      EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
MamI        MfeI        MflI        MluI        MroI        
MunI        NheI        NruI        NsiI        NspV        
PmeI        PvuI        RsrII       SalI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SspI        
SwaI        XbaI        XhoII       


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AclI         1892  AACGTT               AA^CGTT
AcsI         3442  GAATTC               [GA]^AATT[CT]
AgeI         1444  ACCGGT               A^CCGGT
ApoI         3442  GAATTC               [GA]^AATT[CT]
AseI         4533  ATTAAT               AT^TAAT
AsnI         4533  ATTAAT               AT^TAAT
Asp700I       735  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1789  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3266  TGGCCA               TGG^CCA
BbeI         1721  GGCGCC               GGCGC^C
BbrPI        1705  CACGTG               CAC^GTG
BcgI         1898  GCAGCACACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         2918  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2918  GCTCAGC              GC^T[ACGT]AGC
BsaWI        1444  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     3518  TGTACA               T^GTACA
BspHI        1169  TCATGA               T^CATGA
BsrDI        3500  CATTGC               GCAATG(2/0)
BsrGI        3518  TGTACA               T^GTACA
BssSI        1573  CTCGTG               CACGAG(-5/-1)
BstEII       1997  GGTCACC              G^GT[ACGT]ACC
BstPI        1997  GGTCACC              G^GT[ACGT]ACC
BstXI        3046  CCAGAAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        2918  GCTCAGC              GC^T[ACGT]AGC
EcoO65I      1997  GGTCACC              G^GT[ACGT]ACC
EcoRI        3442  GAATTC               G^AATTC
EcoRV        4546  GATATC               GAT^ATC
KasI         1721  GGCGCC               G^GCGCC
MluNI        3266  TGGCCA               TGG^CCA
MscI         3266  TGGCCA               TGG^CCA
NarI         1721  GGCGCC               GG^CGCC
NdeI         3806  CATATG               CA^TATG
NotI            1  GCGGCCGC             GC^GGCCGC
PacI         4534  TTAATTAA             TTAAT^TAA
PaeR7I       1906  CTCGAG               C^TCGAG
PflFI        1789  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1444  ACCGGT               A^CCGGT
PmaCI        1705  CACGTG               CAC^GTG
PmlI         1705  CACGTG               CAC^GTG
PshAI        2992  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4533  ATTAAT               AT^TAAT
Psp1406I     1892  AACGTT               AA^CGTT
RcaI         1169  TCATGA               T^CATGA
SapI         1770  GCTCTTC              GCTCTTC(1/4)
SbfI         1476  CCTGCAGG             CCTGCA^GG
ScaI         4219  AGTACT               AGT^ACT
SexAI        1583  ACCTGGT              A^CC[AT]GGT
SfoI         1721  GGCGCC               GGC^GCC
SmlI         1906  CTCGAG               C^T[CT][GA]AG
Sse8387I     1476  CCTGCAGG             CCTGCA^GG
SspBI        3518  TGTACA               T^GTACA
Tth111I      1789  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1906  CTCGAG               C^TCGAG
XmnI          735  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

ApaI         1853  GGGCCC               GGGCC^C
             2730  GGGCCC              
BbvCI         798  CCTCAGC              CCTCAGC(-5/-2)
             4279  CCTCAGC             
BsaAI         706  TACGTG               [CT]AC^GT[GA]
             1705  CACGTG              
BssHII       1766  GCGCGC               G^CGCGC
             2561  GCGCGC              
DrdI         2154  GACCCCGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2901  GACCTCATGGTC        
KspI           79  CCGCGG               CCGC^GG
             2627  CCGCGG              
NaeI          727  GCCGGC               GCC^GGC
             3110  GCCGGC              
NgoMIV        727  GCCGGC               G^CCGGC
             3110  GCCGGC              
NspI         1843  GCATGC               [GA]CATG^[CT]
             2375  GCATGC              
SacII          79  CCGCGG               CCGC^GG
             2627  CCGCGG              
SphI         1843  GCATGC               GCATG^C
             2375  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       AspHI       
AvaI        AvaII       AviII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XmaCI       XmaI