################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AflIII AhdI AocI Aor51HI AscI Asp718I AspEI AvrII BamHI BciVI BclI BfrI BglII BlnI BsaBI BseAI BsiWI BsmI BspDI BspEI BspLU11I Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MflI MluI MroI MunI NheI NruI NsiI NspV PmeI PvuI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XhoII ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AclI 1892 AACGTT AA^CGTT AcsI 3442 GAATTC [GA]^AATT[CT] AgeI 1444 ACCGGT A^CCGGT ApoI 3442 GAATTC [GA]^AATT[CT] AseI 4533 ATTAAT AT^TAAT AsnI 4533 ATTAAT AT^TAAT Asp700I 735 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1789 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3266 TGGCCA TGG^CCA BbeI 1721 GGCGCC GGCGC^C BbrPI 1705 CACGTG CAC^GTG BcgI 1898 GCAGCACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 2918 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2918 GCTCAGC GC^T[ACGT]AGC BsaWI 1444 ACCGGT [AT]^CCGG[AT] Bsp1407I 3518 TGTACA T^GTACA BspHI 1169 TCATGA T^CATGA BsrDI 3500 CATTGC GCAATG(2/0) BsrGI 3518 TGTACA T^GTACA BssSI 1573 CTCGTG CACGAG(-5/-1) BstEII 1997 GGTCACC G^GT[ACGT]ACC BstPI 1997 GGTCACC G^GT[ACGT]ACC BstXI 3046 CCAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2918 GCTCAGC GC^T[ACGT]AGC EcoO65I 1997 GGTCACC G^GT[ACGT]ACC EcoRI 3442 GAATTC G^AATTC EcoRV 4546 GATATC GAT^ATC KasI 1721 GGCGCC G^GCGCC MluNI 3266 TGGCCA TGG^CCA MscI 3266 TGGCCA TGG^CCA NarI 1721 GGCGCC GG^CGCC NdeI 3806 CATATG CA^TATG NotI 1 GCGGCCGC GC^GGCCGC PacI 4534 TTAATTAA TTAAT^TAA PaeR7I 1906 CTCGAG C^TCGAG PflFI 1789 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1444 ACCGGT A^CCGGT PmaCI 1705 CACGTG CAC^GTG PmlI 1705 CACGTG CAC^GTG PshAI 2992 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4533 ATTAAT AT^TAAT Psp1406I 1892 AACGTT AA^CGTT RcaI 1169 TCATGA T^CATGA SapI 1770 GCTCTTC GCTCTTC(1/4) SbfI 1476 CCTGCAGG CCTGCA^GG ScaI 4219 AGTACT AGT^ACT SexAI 1583 ACCTGGT A^CC[AT]GGT SfoI 1721 GGCGCC GGC^GCC SmlI 1906 CTCGAG C^T[CT][GA]AG Sse8387I 1476 CCTGCAGG CCTGCA^GG SspBI 3518 TGTACA T^GTACA Tth111I 1789 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1906 CTCGAG C^TCGAG XmnI 735 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### ApaI 1853 GGGCCC GGGCC^C 2730 GGGCCC BbvCI 798 CCTCAGC CCTCAGC(-5/-2) 4279 CCTCAGC BsaAI 706 TACGTG [CT]AC^GT[GA] 1705 CACGTG BssHII 1766 GCGCGC G^CGCGC 2561 GCGCGC DrdI 2154 GACCCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2901 GACCTCATGGTC KspI 79 CCGCGG CCGC^GG 2627 CCGCGG NaeI 727 GCCGGC GCC^GGC 3110 GCCGGC NgoMIV 727 GCCGGC G^CCGGC 3110 GCCGGC NspI 1843 GCATGC [GA]CATG^[CT] 2375 GCATGC SacII 79 CCGCGG CCGC^GG 2627 CCGCGG SphI 1843 GCATGC GCATG^C 2375 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI AspHI AvaI AvaII AviII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmaCI XmaI