##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BbeI BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
EcoRI EcoT22I FbaI FseI HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MluNI MroI MscI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI StuI SwaI
TfiI Tsp509I Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 538 CGCG CG^CG
Aor51HI 94 AGCGCT AGC^GCT
AspI 684 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 265 TGCGCA TGC^GCA
BanI 1380 GGCACC G^G[CT][GA]CC
BbvCI 1817 GCTGAGG CCTCAGC(-5/-2)
BsaBI 705 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1808 GAATGC GAATGC(1/-1)
BssSI 372 CTCGTG CACGAG(-5/-1)
BstUI 538 CGCG CG^CG
BstYI 862 AGATCC [GA]^GATC[CT]
DraIII 45 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1681 CGGCCG C^GGCCG
EclXI 1681 CGGCCG C^GGCCG
Eco47III 94 AGCGCT AGC^GCT
Eco52I 1681 CGGCCG C^GGCCG
EcoNI 41 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1089 GATATC GAT^ATC
FspI 265 TGCGCA TGC^GCA
HincII 420 GTCAAC GT[CT]^[GA]AC
HindII 420 GTCAAC GT[CT]^[GA]AC
MamI 705 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 862 AGATCC [GA]^GATC[CT]
MseI 1857 TTAA T^TAA
MvnI 538 CGCG CG^CG
PflFI 684 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 334 AGTACT AGT^ACT
SexAI 46 ACCAGGT A^CC[AT]GGT
SfiI 1469 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 55 CCCGGG CCC^GGG
SphI 1825 GCATGC GCATG^C
Tru9I 1857 TTAA T^TAA
Tth111I 684 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 862 AGATCC [GA]^GATC[CT]
XmaCI 55 CCCGGG C^CCGGG
XmaI 55 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 417 GGCGTC G[GA]^CG[CT]C
1686 GGCGTC
AflIII 424 ACACGT A^C[GA][CT]GT
426 ACGTGT
AocI 530 CCTGAGG CC^T[ACGT]AGG
1410 CCTCAGG
BbrPI 228 CACGTG CAC^GTG
425 CACGTG
BsaAI 228 CACGTG [CT]AC^GT[GA]
425 CACGTG
BsaHI 417 GGCGTC G[GA]^CG[CT]C
1686 GGCGTC
BsrFI 198 GCCGGC [GA]^CCGG[CT]
1683 GCCGGC
BstXI 678 CCAGAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1183 CCATGGACGTGG
Bsu36I 530 CCTGAGG CC^T[ACGT]AGG
1410 CCTCAGG
Cfr10I 198 GCCGGC [GA]^CCGG[CT]
1683 GCCGGC
Eco81I 530 CCTGAGG CC^T[ACGT]AGG
1410 CCTCAGG
EcoT14I 487 CCATGG C^C[AT][AT]GG
1183 CCATGG
Hin1I 417 GGCGTC G[GA]^CG[CT]C
1686 GGCGTC
NaeI 198 GCCGGC GCC^GGC
1683 GCCGGC
NcoI 487 CCATGG C^CATGG
1183 CCATGG
NgoMIV 198 GCCGGC G^CCGGC
1683 GCCGGC
PmaCI 228 CACGTG CAC^GTG
425 CACGTG
PmlI 228 CACGTG CAC^GTG
425 CACGTG
SacI 276 GAGCTC GAGCT^C
725 GAGCTC
SapI 278 GCTCTTC GCTCTTC(1/4)
1505 GCTCTTC
StyI 487 CCATGG C^C[AT][AT]GG
1183 CCATGG
XcmI 487 CCATGGTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1270 CCAGCTGGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI Sse8387I TaqI
TseI Tsp45I TspRI TthHB8I