##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp718I AviII
BbrPI BciVI BclI BfrI BglII
BsiEI BsiWI Bsp1407I BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CpoI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRV
FbaI FspI HincII HindII HpaI
KpnI MfeI MluI MunI NcoI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SexAI SfuI SgrAI SnaBI
SphI SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AhdI 3155 GACACATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3155 GACACATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4225 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3599 CCTAGG C^CTAGG
BbeI 2945 GGCGCC GGCGC^C
BcgI 5026 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3599 CCTAGG C^CTAGG
BlpI 3412 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3412 GCTCAGC GC^T[ACGT]AGC
BsaAI 2969 TACGTG [CT]AC^GT[GA]
BsaBI 2338 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 155 ATCGAT AT^CGAT
BspHI 3624 TCATGA T^CATGA
BssSI 1718 CACGAG CACGAG(-5/-1)
CelII 3412 GCTCAGC GC^T[ACGT]AGC
ClaI 155 ATCGAT AT^CGAT
Eam1105I 3155 GACACATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3673 CCTTTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 5387 ATGCAT ATGCA^T
FseI 777 GGCCGGCC GGCCGG^CC
HindIII 3897 AAGCTT A^AGCTT
KasI 2945 GGCGCC G^GCGCC
KspI 2637 CCGCGG CCGC^GG
MamI 2338 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2945 GGCGCC GG^CGCC
NheI 4662 GCTAGC G^CTAGC
NsiI 5387 ATGCAT ATGCA^T
PflFI 4225 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3624 TCATGA T^CATGA
SacII 2637 CCGCGG CCGC^GG
SbfI 539 CCTGCAGG CCTGCA^GG
SfiI 1195 GGCCCACTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2945 GGCGCC GGC^GCC
SmaI 1964 CCCGGG CCC^GGG
SpeI 4289 ACTAGT A^CTAGT
Sse8387I 539 CCTGCAGG CCTGCA^GG
StuI 1107 AGGCCT AGG^CCT
Tth111I 4225 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1964 CCCGGG C^CCGGG
XmaI 1964 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 200 GTCTAC GT^[AC][GT]AC
3274 GTCTAC
AcyI 495 GGCGTC G[GA]^CG[CT]C
2945 GGCGCC
Alw44I 770 GTGCAC G^TGCAC
2563 GTGCAC
Aor51HI 2043 AGCGCT AGC^GCT
4687 AGCGCT
ApaLI 770 GTGCAC G^TGCAC
2563 GTGCAC
BanI 578 GGCACC G^G[CT][GA]CC
2945 GGCGCC
BglI 1091 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1196 GCCCACTTGGC
BsaHI 495 GGCGTC G[GA]^CG[CT]C
2945 GGCGCC
BsrFI 778 GCCGGC [GA]^CCGG[CT]
2496 GCCGGC
Cfr10I 778 GCCGGC [GA]^CCGG[CT]
2496 GCCGGC
DsaI 1954 CCGTGG C^C[GA][CT]GG
2637 CCGCGG
Eco47III 2043 AGCGCT AGC^GCT
4687 AGCGCT
HgaI 496 GCGTC GACGC(5/10)
2286 GCGTC
Hin1I 495 GGCGTC G[GA]^CG[CT]C
2945 GGCGCC
NaeI 778 GCCGGC GCC^GGC
2496 GCCGGC
NdeI 2309 CATATG CA^TATG
5237 CATATG
NgoMIV 778 GCCGGC G^CCGGC
2496 GCCGGC
XbaI 4134 TCTAGA T^CTAGA
4917 TCTAGA
XcmI 1422 CCAGCACAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1945 CCACAGTGTCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BamHI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI