##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI AseI AsnI AviII BamHI
BbeI BbrPI BfrI BsaAI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI Eco47III EcoRV FseI FspI
HpaI KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 5615 GGTACC G^GTACC
AflIII 4600 ACATGT A^C[GA][CT]GT
AhdI 4259 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1548 GTGCAC G^TGCAC
ApaLI 1548 GTGCAC G^TGCAC
Asp718I 5615 GGTACC G^GTACC
AspEI 4259 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4102 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 462 GTATCC GTATCC(6/5)
BsiEI 2599 CGGCCG CG[GA][CT]^CG
Bsp1407I 2203 TGTACA T^GTACA
BspHI 86 TCATGA T^CATGA
BspLU11I 4600 ACATGT A^CATGT
BsrDI 3995 CATTGC GCAATG(2/0)
BsrGI 2203 TGTACA T^GTACA
BssHII 2955 GCGCGC G^CGCGC
DrdI 520 GACGAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2599 CGGCCG C^GGCCG
Eam1105I 4259 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2599 CGGCCG C^GGCCG
Eco52I 2599 CGGCCG C^GGCCG
EcoRI 1963 GAATTC G^AATTC
EcoT22I 5015 ATGCAT ATGCA^T
KpnI 5615 GGTACC GGTAC^C
NdeI 449 CATATG CA^TATG
NsiI 5015 ATGCAT ATGCA^T
PaeR7I 2622 CTCGAG C^TCGAG
PflFI 4102 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 86 TCATGA T^CATGA
SapI 5378 GCTCTTC GCTCTTC(1/4)
SbfI 1680 CCTGCAGG CCTGCA^GG
SmaI 5424 CCCGGG CCC^GGG
SphI 1465 GCATGC GCATG^C
Sse8387I 1680 CCTGCAGG CCTGCA^GG
SspBI 2203 TGTACA T^GTACA
Tth111I 4102 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2622 CTCGAG C^TCGAG
XmaCI 5424 CCCGGG C^CCGGG
XmaI 5424 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1774 GACGTC GACGT^C
3455 GACGTC
AccI 4256 GTAGAC GT^[AC][GT]AC
4509 GTAGAC
AccII 2956 CGCG CG^CG
3207 CGCG
Asp700I 2417 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3928 GAAAATTTTC
AvrII 564 CCTAGG C^CTAGG
5300 CCTAGG
BcgI 2614 GCACGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3764 GCAGAATCCTCG
BclI 89 TGATCA T^GATCA
5524 TGATCA
BglII 2887 AGATCT A^GATCT
4416 AGATCT
BlnI 564 CCTAGG C^CTAGG
5300 CCTAGG
BsmBI 2625 GAGACG CGTCTC(1/5)
3453 GAGACG
BsrFI 4573 GCCGGT [GA]^CCGG[CT]
5284 GCCGGT
BstUI 2956 CGCG CG^CG
3207 CGCG
Cfr10I 4573 GCCGGT [GA]^CCGG[CT]
5284 GCCGGT
DraI 3950 TTTAAA TTT^AAA
5575 TTTAAA
DraIII 1816 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1875 CACCGAGTG
FbaI 89 TGATCA T^GATCA
5524 TGATCA
HincII 1515 GTCAAC GT[CT]^[GA]AC
3511 GTTGAC
HindII 1515 GTCAAC GT[CT]^[GA]AC
3511 GTTGAC
HindIII 1358 AAGCTT A^AGCTT
1949 AAGCTT
MvnI 2956 CGCG CG^CG
3207 CGCG
NspI 1465 GCATGC [GA]CATG^[CT]
4600 ACATGT
SacI 2271 GAGCTC GAGCT^C
3433 GAGCTC
SfiI 3082 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4940 GGCCAGAGTGGCC
XmnI 2417 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3928 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII