##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI BciVI BfrI BglII BsaBI
BsiWI BspDI BspLU11I BsrDI BssHII
BstBI ClaI CpoI DraI DrdI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI MamI MfeI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SfuI SnaBI SpeI
SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 763 GGTACC G^GTACC
AccIII 6480 TCCGGA T^CCGGA
AflIII 1612 ACGCGT A^C[GA][CT]GT
AhdI 4365 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2846 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 763 GGTACC G^GTACC
AspEI 4365 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5975 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 6697 TGATCA T^GATCA
BsaWI 6480 TCCGGA [AT]^CCGG[AT]
BseAI 6480 TCCGGA T^CCGGA
BsmI 1565 GCATTC GAATGC(1/-1)
BspEI 6480 TCCGGA T^CCGGA
BspHI 2514 TCATGA T^CATGA
Bst1107I 4681 GTATAC GTA^TAC
BstZ17I 4681 GTATAC GTA^TAC
Eam1105I 4365 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 994 GAATTC G^AATTC
FbaI 6697 TGATCA T^GATCA
KpnI 763 GGTACC GGTAC^C
KspI 5477 CCGCGG CCGC^GG
MluI 1612 ACGCGT A^CGCGT
MroI 6480 TCCGGA T^CCGGA
PflFI 5975 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2514 TCATGA T^CATGA
SacII 5477 CCGCGG CCGC^GG
SgrAI 1820 CGCCGGTG C[GA]^CCGG[CT]G
SspI 2812 AATATT AAT^ATT
Tth111I 5975 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5848 TCTAGA T^CTAGA
XmnI 2846 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 4681 GTATAC GT^[AC][GT]AC
6172 GTCTAC
AcsI 994 GAATTC [GA]^AATT[CT]
3598 AAATTT
Aor51HI 6291 AGCGCT AGC^GCT
6456 AGCGCT
ApoI 994 GAATTC [GA]^AATT[CT]
3598 AAATTT
AviII 229 TGCGCA TGC^GCA
3797 TGCGCA
AvrII 5126 CCTAGG C^CTAGG
5644 CCTAGG
BlnI 5126 CCTAGG C^CTAGG
5644 CCTAGG
Bsp1407I 2828 TGTACA T^GTACA
6570 TGTACA
BsrBI 1501 CCGCTC CCGCTC(-3/-3)
4766 CCGCTC
BsrGI 2828 TGTACA T^GTACA
6570 TGTACA
BstEII 5103 GGTAACC G^GT[ACGT]ACC
5518 GGTGACC
BstPI 5103 GGTAACC G^GT[ACGT]ACC
5518 GGTGACC
Eco47III 6291 AGCGCT AGC^GCT
6456 AGCGCT
EcoO65I 5103 GGTAACC G^GT[ACGT]ACC
5518 GGTGACC
FspI 229 TGCGCA TGC^GCA
3797 TGCGCA
NcoI 3120 CCATGG C^CATGG
4532 CCATGG
PaeR7I 1425 CTCGAG C^TCGAG
6395 CTCGAG
PshAI 5212 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6393 GACTCGAGTC
SfiI 89 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5662 GGCCGGGGCGGCC
SspBI 2828 TGTACA T^GTACA
6570 TGTACA
XcmI 688 CCACACTCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6564 CCATTTTGTACATGG
XhoI 1425 CTCGAG C^TCGAG
6395 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI