##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbrPI BcgI BfrI BsaAI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EcoRV EcoT22I FspI
HindIII HpaI KpnI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AccI 4425 GTCTAC GT^[AC][GT]AC
AfaI 394 GTAC GT^AC
AflIII 1386 ACGTGT A^C[GA][CT]GT
AhdI 509 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2711 AGCGCT AGC^GCT
Asp700I 4748 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 509 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 3012 GTATCC GTATCC(6/5)
BglII 258 AGATCT A^GATCT
BsaBI 1081 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3098 CGGCCG CG[GA][CT]^CG
BspHI 5203 TCATGA T^CATGA
EagI 3098 CGGCCG C^GGCCG
Eam1105I 509 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3098 CGGCCG C^GGCCG
Eco47III 2711 AGCGCT AGC^GCT
Eco52I 3098 CGGCCG C^GGCCG
EcoRI 4748 GAATTC G^AATTC
FseI 4084 GGCCGGCC GGCCGG^CC
MamI 1081 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PshAI 1054 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5203 TCATGA T^CATGA
RsaI 394 GTAC GT^AC
SacI 1499 GAGCTC GAGCT^C
XmnI 4748 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AatII 1732 GACGTC GACGT^C
5565 GACGTC
AcsI 4302 AAATTC [GA]^AATT[CT]
4748 GAATTC
Alw44I 2639 GTGCAC G^TGCAC
6344 GTGCAC
ApaLI 2639 GTGCAC G^TGCAC
6344 GTGCAC
ApoI 4302 AAATTC [GA]^AATT[CT]
4748 GAATTC
BclI 597 TGATCA T^GATCA
711 TGATCA
BsmI 952 GAATGC GAATGC(1/-1)
6185 GCATTC
DraIII 284 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
6338 CACACCGTG
FbaI 597 TGATCA T^GATCA
711 TGATCA
HincII 1061 GTCAAC GT[CT]^[GA]AC
5635 GTCAAC
HindII 1061 GTCAAC GT[CT]^[GA]AC
5635 GTCAAC
SapI 1592 GAAGAGC GCTCTTC(1/4)
3280 GAAGAGC
SfiI 2305 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3163 GGCCTCAGGGGCC
SphI 1644 GCATGC GCATG^C
3336 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AccIII AciI AcyI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI