##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AcsI AgeI ApoI
AscI AseI AsnI Asp700I BbrPI
BsiWI Bsp1407I BspDI BspHI BsrGI
Bst1107I BstZ17I ClaI CpoI EcoRI
EcoRV FseI HindIII MfeI MluI
MunI NdeI NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SnaBI SpeI SspBI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2401 GGTACC G^GTACC
AccI 1080 GTCTAC GT^[AC][GT]AC
AccIII 2344 TCCGGA T^CCGGA
AflIII 1702 ACATGT A^C[GA][CT]GT
AhdI 723 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2401 GGTACC G^GTACC
AspEI 723 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2407 GTATCC GTATCC(6/5)
BglII 1624 AGATCT A^GATCT
BsaBI 2342 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2344 TCCGGA T^CCGGA
BsiEI 2833 CGGCCG CG[GA][CT]^CG
BspEI 2344 TCCGGA T^CCGGA
BspLU11I 1702 ACATGT A^CATGT
BssHII 3292 GCGCGC G^CGCGC
BstBI 1644 TTCGAA TT^CGAA
DraI 6265 TTTAAA TTT^AAA
DraIII 4230 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3422 GACGCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2833 CGGCCG C^GGCCG
Eam1105I 723 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2833 CGGCCG C^GGCCG
Eco52I 2833 CGGCCG C^GGCCG
EcoT22I 1838 ATGCAT ATGCA^T
HincII 771 GTTAAC GT[CT]^[GA]AC
HindII 771 GTTAAC GT[CT]^[GA]AC
HpaI 771 GTTAAC GTT^AAC
KpnI 2401 GGTACC GGTAC^C
MamI 2342 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2344 TCCGGA T^CCGGA
NsiI 1838 ATGCAT ATGCA^T
NspV 1644 TTCGAA TT^CGAA
ScaI 793 AGTACT AGT^ACT
SfuI 1644 TTCGAA TT^CGAA
SgrAI 3054 CGCCGGCG C[GA]^CCGG[CT]G
SphI 5827 GCATGC GCATG^C
TfiI 660 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 44 CTTAAG C^TTAAG
5748 CTTAAG
AocI 932 CCTGAGG CC^T[ACGT]AGG
4994 CCTGAGG
Aor51HI 535 AGCGCT AGC^GCT
2947 AGCGCT
AvrII 1241 CCTAGG C^CTAGG
6231 CCTAGG
BbeI 1287 GGCGCC GGCGC^C
4186 GGCGCC
BcgI 3926 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4364 CGAAGCAGATGC
BfrI 44 CTTAAG C^TTAAG
5748 CTTAAG
BlnI 1241 CCTAGG C^CTAGG
6231 CCTAGG
BsaAI 348 TACGTG [CT]AC^GT[GA]
1455 TACGTG
BsrDI 2323 GCAATG GCAATG(2/0)
5062 GCAATG
BstXI 71 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1052 CCAGTTGCCTGG
Bsu36I 932 CCTGAGG CC^T[ACGT]AGG
4994 CCTGAGG
Eco47III 535 AGCGCT AGC^GCT
2947 AGCGCT
Eco81I 932 CCTGAGG CC^T[ACGT]AGG
4994 CCTGAGG
KasI 1287 GGCGCC G^GCGCC
4186 GGCGCC
KspI 1006 CCGCGG CCGC^GG
3709 CCGCGG
NaeI 3055 GCCGGC GCC^GGC
5332 GCCGGC
NarI 1287 GGCGCC GG^CGCC
4186 GGCGCC
NgoMIV 3055 GCCGGC G^CCGGC
5332 GCCGGC
PshAI 2039 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2864 GACGCACGTC
SacII 1006 CCGCGG CCGC^GG
3709 CCGCGG
SexAI 4780 ACCAGGT A^CC[AT]GGT
5836 ACCTGGT
SfoI 1287 GGCGCC GGC^GCC
4186 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AspI AvaI AvaII AviII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfiI
SmaI SmlI Sse8387I StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI