##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BlnI BsaBI BsaHI
BsiWI BspDI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FseI FspI Hin1I HpaI KasI
KpnI MamI MfeI MluI MunI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 4384 GTCTAC GT^[AC][GT]AC
AccIII 2676 TCCGGA T^CCGGA
AflII 1868 CTTAAG C^TTAAG
Aor51HI 3572 AGCGCT AGC^GCT
BamHI 4002 GGATCC G^GATCC
BcgI 2263 GCAGGAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1868 CTTAAG C^TTAAG
BsaWI 2676 TCCGGA [AT]^CCGG[AT]
BseAI 2676 TCCGGA T^CCGGA
BsiEI 857 CGGTCG CG[GA][CT]^CG
BsmBI 140 CGTCTC CGTCTC(1/5)
Bsp1407I 999 TGTACA T^GTACA
BspEI 2676 TCCGGA T^CCGGA
BspLU11I 325 ACATGT A^CATGT
BsrGI 999 TGTACA T^GTACA
EarI 4098 CTCTTC CTCTTC(1/4)
Eco47III 3572 AGCGCT AGC^GCT
EcoRI 4028 GAATTC G^AATTC
HgaI 2269 GCGTC GACGC(5/10)
Ksp632I 4098 CTCTTC CTCTTC(1/4)
KspI 4209 CCGCGG CCGC^GG
MroI 2676 TCCGGA T^CCGGA
NdeI 4070 CATATG CA^TATG
NspI 325 ACATGT [GA]CATG^[CT]
SacII 4209 CCGCGG CCGC^GG
SapI 4097 GCTCTTC GCTCTTC(1/4)
SfiI 3671 GGCCCACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 999 TGTACA T^GTACA
XbaI 1503 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 2732 CGCG CG^CG
4210 CGCG
AflIII 325 ACATGT A^C[GA][CT]GT
996 ACGTGT
BbrPI 5084 CACGTG CAC^GTG
5403 CACGTG
BbvCI 3534 GCTGAGG CCTCAGC(-5/-2)
5726 GCTGAGG
BglII 2418 AGATCT A^GATCT
5090 AGATCT
BsaAI 5084 CACGTG [CT]AC^GT[GA]
5403 CACGTG
BsrBI 1186 GAGCGG CCGCTC(-3/-3)
3541 GAGCGG
BsrDI 1008 CATTGC GCAATG(2/0)
2695 GCAATG
BssSI 3798 CACGAG CACGAG(-5/-1)
3470 CTCGTG
BstEII 3188 GGTGACC G^GT[ACGT]ACC
4201 GGTGACC
BstPI 3188 GGTGACC G^GT[ACGT]ACC
4201 GGTGACC
BstUI 2732 CGCG CG^CG
4210 CGCG
DraIII 2109 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3501 CACCATGTG
EcoO65I 3188 GGTGACC G^GT[ACGT]ACC
4201 GGTGACC
HaeII 585 AGCGCC [GA]GCGC^[CT]
3572 AGCGCT
HincII 1309 GTTGAC GT[CT]^[GA]AC
1374 GTCAAC
HindII 1309 GTTGAC GT[CT]^[GA]AC
1374 GTCAAC
HindIII 2192 AAGCTT A^AGCTT
2857 AAGCTT
MvnI 2732 CGCG CG^CG
4210 CGCG
NaeI 2239 GCCGGC GCC^GGC
3668 GCCGGC
NcoI 1479 CCATGG C^CATGG
4307 CCATGG
NgoMIV 2239 GCCGGC G^CCGGC
3668 GCCGGC
PmaCI 5084 CACGTG CAC^GTG
5403 CACGTG
PmlI 5084 CACGTG CAC^GTG
5403 CACGTG
PshAI 2996 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3864 GACAGAGGTC
SexAI 2110 ACCAGGT A^CC[AT]GGT
4917 ACCTGGT
SmaI 572 CCCGGG CCC^GGG
863 CCCGGG
SwaI 5806 ATTTAAAT ATTT^AAAT
5828 ATTTAAAT
XcmI 471 CCAGCTTCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3674 CCACGGGGCCGCTGG
XmaCI 572 CCCGGG C^CCGGG
863 CCCGGG
XmaI 572 CCCGGG C^CCGGG
863 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI