##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccI AgeI AscI
Asp718I AviII AvrII BamHI BbrPI
BciVI BglII BlnI BsaBI BsiWI
BspDI BsrBI BsrFI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SfiI SfuI
SgrAI SmaI SnaBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 5502 TCCGGA T^CCGGA
Alw44I 1737 GTGCAC G^TGCAC
Aor51HI 1122 AGCGCT AGC^GCT
ApaI 3318 GGGCCC GGGCC^C
ApaLI 1737 GTGCAC G^TGCAC
Asp700I 5077 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 5751 CTCGAG C^[CT]CG[GA]G
BbeI 4920 GGCGCC GGCGC^C
BclI 2333 TGATCA T^GATCA
BsaAI 2116 CACGTA [CT]AC^GT[GA]
BseAI 5502 TCCGGA T^CCGGA
BsiEI 4679 CGACCG CG[GA][CT]^CG
BsoBI 5751 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2010 TGTACA T^GTACA
BspEI 5502 TCCGGA T^CCGGA
BspHI 2097 TCATGA T^CATGA
BspLU11I 4028 ACATGT A^CATGT
BsrDI 3571 CATTGC GCAATG(2/0)
BsrGI 2010 TGTACA T^GTACA
Eco47III 1122 AGCGCT AGC^GCT
EcoRV 6037 GATATC GAT^ATC
FbaI 2333 TGATCA T^GATCA
KasI 4920 GGCGCC G^GCGCC
MroI 5502 TCCGGA T^CCGGA
NarI 4920 GGCGCC GG^CGCC
NcoI 3766 CCATGG C^CATGG
NdeI 2493 CATATG CA^TATG
PaeR7I 5751 CTCGAG C^TCGAG
RcaI 2097 TCATGA T^CATGA
SapI 2922 GAAGAGC GCTCTTC(1/4)
SbfI 5513 CCTGCAGG CCTGCA^GG
SexAI 5391 ACCAGGT A^CC[AT]GGT
SfoI 4920 GGCGCC GGC^GCC
SpeI 3935 ACTAGT A^CTAGT
Sse8387I 5513 CCTGCAGG CCTGCA^GG
SspBI 2010 TGTACA T^GTACA
XhoI 5751 CTCGAG C^TCGAG
XmnI 5077 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AclI 129 AACGTT AA^CGTT
2232 AACGTT
AcyI 4920 GGCGCC G[GA]^CG[CT]C
5582 GGCGTC
AflII 2287 CTTAAG C^TTAAG
2734 CTTAAG
AflIII 2115 ACACGT A^C[GA][CT]GT
4028 ACATGT
AhdI 1164 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1361 GACCCCAAGTC
AseI 2578 ATTAAT AT^TAAT
2755 ATTAAT
AsnI 2578 ATTAAT AT^TAAT
2755 ATTAAT
AspEI 1164 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1361 GACCCCAAGTC
AspI 1861 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3656 GACAAGGTC
BcgI 1775 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4462 GCATGGGGCTCG
BcnI 3641 CCCGG CC^[GC]GG
5230 CCGGG
BfrI 2287 CTTAAG C^TTAAG
2734 CTTAAG
BlpI 4390 GCTCAGC GC^T[ACGT]AGC
5672 GCTGAGC
Bpu1102I 4390 GCTCAGC GC^T[ACGT]AGC
5672 GCTGAGC
BsaHI 4920 GGCGCC G[GA]^CG[CT]C
5582 GGCGTC
BsaWI 523 ACCGGA [AT]^CCGG[AT]
5502 TCCGGA
BsmBI 2983 GAGACG CGTCTC(1/5)
4620 GAGACG
BssHII 64 GCGCGC G^CGCGC
66 GCGCGC
BstAPI 2653 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4949 GCAGAAGCTGC
CelII 4390 GCTCAGC GC^T[ACGT]AGC
5672 GCTGAGC
Eam1105I 1164 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1361 GACCCCAAGTC
Hin1I 4920 GGCGCC G[GA]^CG[CT]C
5582 GGCGTC
MslI 4823 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
6023 CATGTCAGTG
NciI 3641 CCCGG CC^[GC]GG
5230 CCGGG
PflFI 1861 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3656 GACAAGGTC
PshBI 2578 ATTAAT AT^TAAT
2755 ATTAAT
Psp1406I 129 AACGTT AA^CGTT
2232 AACGTT
SacI 866 GAGCTC GAGCT^C
4892 GAGCTC
ScaI 2761 AGTACT AGT^ACT
5981 AGTACT
SphI 201 GCATGC GCATG^C
1973 GCATGC
Tth111I 1861 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3656 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII