##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AocI ApoI AseI AsnI
Asp718I BclI BfrI BglII BsaBI
BsiWI BspDI BspLU11I BstBI BstEII
BstPI Bsu36I ClaI CpoI DrdI
Eco81I EcoO65I EcoRI EcoRV FbaI
HindIII HpaI KpnI MamI MfeI
MluI MunI NdeI NotI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SfiI SfuI SgrAI SpeI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 5057 ACGTGT A^C[GA][CT]GT
AhdI 5711 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2000 GTGCAC G^TGCAC
Aor51HI 2902 AGCGCT AGC^GCT
ApaLI 2000 GTGCAC G^TGCAC
AscI 1225 GGCGCGCC GG^CGCGCC
Asp700I 5622 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5711 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4149 TGCGCA TGC^GCA
AvrII 5042 CCTAGG C^CTAGG
BamHI 5132 GGATCC G^GATCC
BbsI 3038 GTCTTC GAAGAC(2/6)
BcgI 684 GCAGCGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3192 GTATCC GTATCC(6/5)
BlnI 5042 CCTAGG C^CTAGG
BpuAI 3038 GTCTTC GAAGAC(2/6)
BsmBI 4751 GAGACG CGTCTC(1/5)
Bsp1407I 1851 TGTACA T^GTACA
BspHI 1380 TCATGA T^CATGA
BsrDI 4514 CATTGC GCAATG(2/0)
BsrGI 1851 TGTACA T^GTACA
BssHII 1226 GCGCGC G^CGCGC
BssSI 4415 CTCGTG CACGAG(-5/-1)
Bst1107I 4670 GTATAC GTA^TAC
BstZ17I 4670 GTATAC GTA^TAC
DraI 3566 TTTAAA TTT^AAA
Eam1105I 5711 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2902 AGCGCT AGC^GCT
EcoT22I 3493 ATGCAT ATGCA^T
FseI 441 GGCCGGCC GGCCGG^CC
FspI 4149 TGCGCA TGC^GCA
HincII 1097 GTCAAC GT[CT]^[GA]AC
HindII 1097 GTCAAC GT[CT]^[GA]AC
KspI 1050 CCGCGG CCGC^GG
NheI 5490 GCTAGC G^CTAGC
NruI 3288 TCGCGA TCG^CGA
NsiI 3493 ATGCAT ATGCA^T
RcaI 1380 TCATGA T^CATGA
SacII 1050 CCGCGG CCGC^GG
ScaI 4899 AGTACT AGT^ACT
SnaBI 4673 TACGTA TAC^GTA
SphI 200 GCATGC GCATG^C
SspBI 1851 TGTACA T^GTACA
XbaI 3901 TCTAGA T^CTAGA
XmnI 5622 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1829 GTCTAC GT^[AC][GT]AC
4670 GTATAC
AccIII 2953 TCCGGA T^CCGGA
5880 TCCGGA
AspI 331 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4753 GACGGAGTC
BbrPI 2627 CACGTG CAC^GTG
5611 CACGTG
BsaWI 2953 TCCGGA [AT]^CCGG[AT]
5880 TCCGGA
BseAI 2953 TCCGGA T^CCGGA
5880 TCCGGA
BsiEI 2773 CGGCCG CG[GA][CT]^CG
3162 CGGCCG
BspEI 2953 TCCGGA T^CCGGA
5880 TCCGGA
BsrBI 1028 CCGCTC CCGCTC(-3/-3)
415 GAGCGG
EagI 2773 CGGCCG C^GGCCG
3162 CGGCCG
EclXI 2773 CGGCCG C^GGCCG
3162 CGGCCG
Eco52I 2773 CGGCCG C^GGCCG
3162 CGGCCG
MroI 2953 TCCGGA T^CCGGA
5880 TCCGGA
PflFI 331 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4753 GACGGAGTC
PmaCI 2627 CACGTG CAC^GTG
5611 CACGTG
PmlI 2627 CACGTG CAC^GTG
5611 CACGTG
SexAI 3389 ACCTGGT A^CC[AT]GGT
5115 ACCTGGT
SspI 4731 AATATT AAT^ATT
5303 AATATT
TfiI 1742 GAATC G^A[AT]TC
5868 GATTC
Tth111I 331 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4753 GACGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI