##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AclI AflIII AgeI AseI AsnI
AviII BamHI BbeI BsaBI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssSI
ClaI CpoI DrdI EcoRV FseI
FspI HindIII HpaI KasI KspI
MamI MluI NarI NheI NotI
NruI PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SnaBI Sse8387I SspBI SwaI XcmI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 5610 GGTACC G^GTACC
AccIII 5415 TCCGGA T^CCGGA
AhdI 3246 GACTTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5480 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4446 AGCGCT AGC^GCT
AscI 180 GGCGCGCC GG^CGCGCC
Asp718I 5610 GGTACC G^GTACC
AspEI 3246 GACTTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 5367 CACGTG CAC^GTG
BciVI 6095 GTATCC GTATCC(6/5)
BclI 342 TGATCA T^GATCA
BlpI 5824 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5824 GCTCAGC GC^T[ACGT]AGC
BsaI 5151 GGTCTC GGTCTC(1/5)
BsaWI 5415 TCCGGA [AT]^CCGG[AT]
BseAI 5415 TCCGGA T^CCGGA
BsmI 4548 GAATGC GAATGC(1/-1)
BspEI 5415 TCCGGA T^CCGGA
BspHI 1667 TCATGA T^CATGA
BsrDI 500 GCAATG GCAATG(2/0)
BsrFI 122 GCCGGC [GA]^CCGG[CT]
BssHII 181 GCGCGC G^CGCGC
Bst1107I 6462 GTATAC GTA^TAC
BstEII 1254 GGTCACC G^GT[ACGT]ACC
BstPI 1254 GGTCACC G^GT[ACGT]ACC
BstZ17I 6462 GTATAC GTA^TAC
Bsu36I 5480 CCTCAGG CC^T[ACGT]AGG
CelII 5824 GCTCAGC GC^T[ACGT]AGC
Cfr10I 122 GCCGGC [GA]^CCGG[CT]
DraIII 5364 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3246 GACTTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4446 AGCGCT AGC^GCT
Eco81I 5480 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1254 GGTCACC G^GT[ACGT]ACC
EcoRI 2090 GAATTC G^AATTC
EcoT22I 3536 ATGCAT ATGCA^T
FbaI 342 TGATCA T^GATCA
HaeII 4446 AGCGCT [GA]GCGC^[CT]
HincII 2437 GTTGAC GT[CT]^[GA]AC
HindII 2437 GTTGAC GT[CT]^[GA]AC
KpnI 5610 GGTACC GGTAC^C
MroI 5415 TCCGGA T^CCGGA
NaeI 122 GCCGGC GCC^GGC
NdeI 1961 CATATG CA^TATG
NgoMIV 122 GCCGGC G^CCGGC
NsiI 3536 ATGCAT ATGCA^T
PaeR7I 5869 CTCGAG C^TCGAG
PflMI 852 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5367 CACGTG CAC^GTG
PmlI 5367 CACGTG CAC^GTG
RcaI 1667 TCATGA T^CATGA
SpeI 4459 ACTAGT A^CTAGT
SphI 6062 GCATGC GCATG^C
Van91I 852 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 5869 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 8 CGCG CG^CG
182 CGCG
Alw44I 2969 GTGCAC G^TGCAC
5370 GTGCAC
ApaLI 2969 GTGCAC G^TGCAC
5370 GTGCAC
Asp700I 1266 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5661 GAATTTATTC
AspI 4782 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4957 GACAGAGTC
AvrII 1621 CCTAGG C^CTAGG
2881 CCTAGG
BalI 194 TGGCCA TGG^CCA
467 TGGCCA
BbsI 769 GAAGAC GAAGAC(2/6)
1266 GAAGAC
BcgI 5039 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5861 GCAGGTCCCTCG
BglI 122 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
469 GCCAGTTTGGC
BglII 3030 AGATCT A^GATCT
5272 AGATCT
BlnI 1621 CCTAGG C^CTAGG
2881 CCTAGG
BpuAI 769 GAAGAC GAAGAC(2/6)
1266 GAAGAC
BsgI 683 CTGCAC GTGCAG(16/14)
3173 GTGCAG
BsiEI 124 CGGCCG CG[GA][CT]^CG
214 CGGCCG
BstBI 1455 TTCGAA TT^CGAA
2487 TTCGAA
BstUI 8 CGCG CG^CG
182 CGCG
BstXI 2779 CCAGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3967 CCATTTTAGTGG
EagI 124 CGGCCG C^GGCCG
214 CGGCCG
EclXI 124 CGGCCG C^GGCCG
214 CGGCCG
Eco52I 124 CGGCCG C^GGCCG
214 CGGCCG
EcoNI 192 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5785 CCTGCGGCAGG
MluNI 194 TGGCCA TGG^CCA
467 TGGCCA
MscI 194 TGGCCA TGG^CCA
467 TGGCCA
MvnI 8 CGCG CG^CG
182 CGCG
NcoI 4298 CCATGG C^CATGG
6175 CCATGG
NspV 1455 TTCGAA TT^CGAA
2487 TTCGAA
PflFI 4782 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4957 GACAGAGTC
SapI 117 GAAGAGC GCTCTTC(1/4)
4732 GCTCTTC
ScaI 5876 AGTACT AGT^ACT
6335 AGTACT
SfuI 1455 TTCGAA TT^CGAA
2487 TTCGAA
SmaI 5027 CCCGGG CCC^GGG
6028 CCCGGG
Tth111I 4782 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4957 GACAGAGTC
XbaI 4033 TCTAGA T^CTAGA
5275 TCTAGA
XmaCI 5027 CCCGGG C^CCGGG
6028 CCCGGG
XmaI 5027 CCCGGG C^CCGGG
6028 CCCGGG
XmnI 1266 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5661 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AatII AccI AciI AcsI AcyI
AfaI AflII AluI AlwI AlwNI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BfrI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII