##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI BamHI BbrPI
BcgI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRV FseI HpaI
MamI MluI MroI NaeI NgoMIV
NheI NotI NruI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SalI SbfI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 4682 GACGTC GACGT^C
Acc65I 5185 GGTACC G^GTACC
AclI 1491 AACGTT AA^CGTT
AflIII 5534 ACATGT A^C[GA][CT]GT
AgeI 990 ACCGGT A^CCGGT
ApaI 2366 GGGCCC GGGCC^C
Asp718I 5185 GGTACC G^GTACC
AviII 100 TGCGCA TGC^GCA
BalI 775 TGGCCA TGG^CCA
BciVI 5915 GTATCC GTATCC(6/5)
BglI 1535 GCCGCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5923 AGATCT A^GATCT
BsaAI 3881 CACGTA [CT]AC^GT[GA]
BsiEI 988 CGACCG CG[GA][CT]^CG
BsmBI 149 GAGACG CGTCTC(1/5)
BspLU11I 5534 ACATGT A^CATGT
BspMI 4349 GCAGGT ACCTGC(4/8)
BstEII 461 GGTGACC G^GT[ACGT]ACC
BstPI 461 GGTGACC G^GT[ACGT]ACC
BstXI 5454 CCAACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 5685 TTTAAA TTT^AAA
EcoO65I 461 GGTGACC G^GT[ACGT]ACC
EcoRI 2744 GAATTC G^AATTC
FspI 100 TGCGCA TGC^GCA
KpnI 5185 GGTACC GGTAC^C
KspI 554 CCGCGG CCGC^GG
MluNI 775 TGGCCA TGG^CCA
MscI 775 TGGCCA TGG^CCA
NcoI 3217 CCATGG C^CATGG
PaeR7I 2874 CTCGAG C^TCGAG
PinAI 990 ACCGGT A^CCGGT
Psp1406I 1491 AACGTT AA^CGTT
SacII 554 CCGCGG CCGC^GG
SexAI 5193 ACCTGGT A^CC[AT]GGT
SgrAI 881 CACCGGCG C[GA]^CCGG[CT]G
SspI 5569 AATATT AAT^ATT
XhoI 2874 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2285 GTCTAC GT^[AC][GT]AC
2395 GTCTAC
AflII 4928 CTTAAG C^TTAAG
5821 CTTAAG
AvaI 92 CCCGAG C^[CT]CG[GA]G
2874 CTCGAG
AvrII 4781 CCTAGG C^CTAGG
5896 CCTAGG
BclI 4572 TGATCA T^GATCA
5132 TGATCA
BfrI 4928 CTTAAG C^TTAAG
5821 CTTAAG
BlnI 4781 CCTAGG C^CTAGG
5896 CCTAGG
BsaWI 477 ACCGGA [AT]^CCGG[AT]
990 ACCGGT
BsoBI 92 CCCGAG C^[CT]CG[GA]G
2874 CTCGAG
BspHI 2953 TCATGA T^CATGA
5092 TCATGA
BsrDI 1328 GCAATG GCAATG(2/0)
2820 GCAATG
BsrFI 882 ACCGGC [GA]^CCGG[CT]
990 ACCGGT
BssSI 507 CACGAG CACGAG(-5/-1)
2787 CTCGTG
Cfr10I 882 ACCGGC [GA]^CCGG[CT]
990 ACCGGT
DraIII 914 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1445 CACAGTGTG
EcoT22I 1089 ATGCAT ATGCA^T
1400 ATGCAT
FbaI 4572 TGATCA T^GATCA
5132 TGATCA
HgaI 790 GACGC GACGC(5/10)
2005 GCGTC
HincII 3386 GTTGAC GT[CT]^[GA]AC
3857 GTCAAC
HindII 3386 GTTGAC GT[CT]^[GA]AC
3857 GTCAAC
MfeI 1314 CAATTG C^AATTG
3231 CAATTG
MunI 1314 CAATTG C^AATTG
3231 CAATTG
NsiI 1089 ATGCAT ATGCA^T
1400 ATGCAT
NspI 4959 GCATGT [GA]CATG^[CT]
5534 ACATGT
PpuMI 3917 GGGTCCT [GA]G^G[AT]CC[CT]
5848 GGGTCCC
PvuII 3464 CAGCTG CAG^CTG
4077 CAGCTG
RcaI 2953 TCATGA T^CATGA
5092 TCATGA
SacI 236 GAGCTC GAGCT^C
2078 GAGCTC
ScaI 4668 AGTACT AGT^ACT
5417 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI ApoI
Asp700I AspEI AspHI AspI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeI
NdeII NlaIII NlaIV PflFI PflMI
PleI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI