##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
BamHI BbrPI BclI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI KspI MluI NaeI
NdeI NgoMIV NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SgrAI SnaBI SpeI
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2701 GGTACC G^GTACC
AccI 114 GTAGAC GT^[AC][GT]AC
AccII 4191 CGCG CG^CG
AccIII 1783 TCCGGA T^CCGGA
AflII 5779 CTTAAG C^TTAAG
Asp718I 2701 GGTACC G^GTACC
AspI 2668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5150 TGCGCA TGC^GCA
BbeI 2304 GGCGCC GGCGC^C
BfrI 5779 CTTAAG C^TTAAG
BsaAI 1797 CACGTA [CT]AC^GT[GA]
BsaWI 1783 TCCGGA [AT]^CCGG[AT]
BseAI 1783 TCCGGA T^CCGGA
BspEI 1783 TCCGGA T^CCGGA
BspHI 2110 TCATGA T^CATGA
BsrDI 3482 CATTGC GCAATG(2/0)
BssSI 5019 CTCGTG CACGAG(-5/-1)
BstBI 2273 TTCGAA TT^CGAA
BstUI 4191 CGCG CG^CG
FspI 5150 TGCGCA TGC^GCA
HpaI 449 GTTAAC GTT^AAC
KasI 2304 GGCGCC G^GCGCC
KpnI 2701 GGTACC GGTAC^C
MfeI 2049 CAATTG C^AATTG
MroI 1783 TCCGGA T^CCGGA
MunI 2049 CAATTG C^AATTG
MvnI 4191 CGCG CG^CG
NarI 2304 GGCGCC GG^CGCC
NcoI 4015 CCATGG C^CATGG
NheI 5619 GCTAGC G^CTAGC
NspV 2273 TTCGAA TT^CGAA
PaeR7I 516 CTCGAG C^TCGAG
PflFI 2668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2110 TCATGA T^CATGA
SbfI 671 CCTGCAGG CCTGCA^GG
SexAI 6177 ACCAGGT A^CC[AT]GGT
SfoI 2304 GGCGCC GGC^GCC
SfuI 2273 TTCGAA TT^CGAA
SmaI 4425 CCCGGG CCC^GGG
SphI 2388 GCATGC GCATG^C
Sse8387I 671 CCTGCAGG CCTGCA^GG
SspI 1961 AATATT AAT^ATT
Tth111I 2668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 516 CTCGAG C^TCGAG
XmaCI 4425 CCCGGG C^CCGGG
XmaI 4425 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 2266 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5998 GAATCTTTTC
AvrII 304 CCTAGG C^CTAGG
6444 CCTAGG
BcgI 221 CGACAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1827 GCAGTGCAGTCG
BciVI 2224 GTATCC GTATCC(6/5)
2986 GTATCC
BglII 396 AGATCT A^GATCT
4237 AGATCT
BlnI 304 CCTAGG C^CTAGG
6444 CCTAGG
BsaBI 908 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6544 GATTCTCATC
BstAPI 4752 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4936 GCAGGCGGTGC
DraI 6726 TTTAAA TTT^AAA
6738 TTTAAA
DrdI 2996 GACTCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6080 GACATTTGGGTC
FauI 4159 GCGGG CCCGC(4/6)
4444 GCGGG
HaeII 2304 GGCGCC [GA]GCGC^[CT]
4072 GGCGCT
HindIII 129 AAGCTT A^AGCTT
3158 AAGCTT
MamI 908 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6544 GATTCTCATC
PshAI 13 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1754 GACATCAGTC
SapI 995 GAAGAGC GCTCTTC(1/4)
1350 GCTCTTC
ScaI 2975 AGTACT AGT^ACT
5636 AGTACT
XbaI 188 TCTAGA T^CTAGA
6144 TCTAGA
XmnI 2266 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5998 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII