################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII AclI AflII AhdI Alw44I ApaLI AscI AspEI AviII AvrII BamHI BbeI BbvCI BclI BfrI BglI BglII BlnI BsaBI BsmI BspLU11I BssHII BstBI BstEII BstPI CpoI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI MamI MluI NarI NheI NotI NruI NsiI NspV PacI PmeI PshAI Psp1406I PvuI RsrII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1685 GGTACC G^GTACC AccIII 4736 TCCGGA T^CCGGA AcyI 156 GACGCC G[GA]^CG[CT]C AgeI 884 ACCGGT A^CCGGT Aor51HI 1804 AGCGCT AGC^GCT ApaI 1830 GGGCCC GGGCC^C Asp700I 2672 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1685 GGTACC G^GTACC BbrPI 1959 CACGTG CAC^GTG BcgI 534 GCAAAGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 156 GACGCC G[GA]^CG[CT]C BseAI 4736 TCCGGA T^CCGGA BsiWI 5412 CGTACG C^GTACG BspEI 4736 TCCGGA T^CCGGA BspHI 1295 TCATGA T^CATGA Bst1107I 2164 GTATAC GTA^TAC BstAPI 5142 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2164 GTATAC GTA^TAC DrdI 469 GACGGAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1804 AGCGCT AGC^GCT EcoNI 2868 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 156 GACGCC G[GA]^CG[CT]C KpnI 1685 GGTACC GGTAC^C KspI 2686 CCGCGG CCGC^GG MroI 4736 TCCGGA T^CCGGA NaeI 2807 GCCGGC GCC^GGC NdeI 5307 CATATG CA^TATG NgoMIV 2807 GCCGGC G^CCGGC PaeR7I 397 CTCGAG C^TCGAG PinAI 884 ACCGGT A^CCGGT PmaCI 1959 CACGTG CAC^GTG PmlI 1959 CACGTG CAC^GTG RcaI 1295 TCATGA T^CATGA SacII 2686 CCGCGG CCGC^GG ScaI 6231 AGTACT AGT^ACT SexAI 821 ACCAGGT A^CC[AT]GGT StuI 139 AGGCCT AGG^CCT XbaI 3131 TCTAGA T^CTAGA XcmI 1864 CCAAAACCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 397 CTCGAG C^TCGAG XmnI 2672 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 2164 GTATAC GT^[AC][GT]AC 5661 GTCTAC AflIII 5768 ACACGT A^C[GA][CT]GT 6000 ACACGT AocI 4047 CCTGAGG CC^T[ACGT]AGG 4684 CCTCAGG AseI 873 ATTAAT AT^TAAT 6336 ATTAAT AsnI 873 ATTAAT AT^TAAT 6336 ATTAAT BciVI 2034 GGATAC GTATCC(6/5) 4728 GTATCC BlpI 2333 GCTGAGC GC^T[ACGT]AGC 2382 GCTCAGC Bpu1102I 2333 GCTGAGC GC^T[ACGT]AGC 2382 GCTCAGC BsaAI 1959 CACGTG [CT]AC^GT[GA] 6001 CACGTA BsiEI 319 CGGCCG CG[GA][CT]^CG 366 CGGCCG Bsp1407I 1519 TGTACA T^GTACA 2290 TGTACA BsrGI 1519 TGTACA T^GTACA 2290 TGTACA Bsu36I 4047 CCTGAGG CC^T[ACGT]AGG 4684 CCTCAGG CelII 2333 GCTGAGC GC^T[ACGT]AGC 2382 GCTCAGC DraIII 820 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2261 CACCATGTG EagI 319 CGGCCG C^GGCCG 366 CGGCCG EclXI 319 CGGCCG C^GGCCG 366 CGGCCG Eco52I 319 CGGCCG C^GGCCG 366 CGGCCG Eco81I 4047 CCTGAGG CC^T[ACGT]AGG 4684 CCTCAGG HincII 1905 GTCAAC GT[CT]^[GA]AC 3724 GTCAAC HindII 1905 GTCAAC GT[CT]^[GA]AC 3724 GTCAAC MfeI 1387 CAATTG C^AATTG 4993 CAATTG MunI 1387 CAATTG C^AATTG 4993 CAATTG NcoI 426 CCATGG C^CATGG 5742 CCATGG PflMI 2505 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2630 CCAGTTTGTGG PshBI 873 ATTAAT AT^TAAT 6336 ATTAAT SphI 4509 GCATGC GCATG^C 5574 GCATGC SspBI 1519 TGTACA T^GTACA 2290 TGTACA Van91I 2505 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2630 CCAGTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmaCI XmaI