##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AclI        AflII       AhdI        Alw44I      
ApaLI       AscI        AspEI       AviII       AvrII       
BamHI       BbeI        BbvCI       BclI        BfrI        
BglI        BglII       BlnI        BsaBI       BsmI        
BspLU11I    BssHII      BstBI       BstEII      BstPI       
CpoI        Eam1105I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HpaI        
KasI        MamI        MluI        NarI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
SwaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       1685  GGTACC               G^GTACC
AccIII       4736  TCCGGA               T^CCGGA
AcyI          156  GACGCC               G[GA]^CG[CT]C
AgeI          884  ACCGGT               A^CCGGT
Aor51HI      1804  AGCGCT               AGC^GCT
ApaI         1830  GGGCCC               GGGCC^C
Asp700I      2672  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1685  GGTACC               G^GTACC
BbrPI        1959  CACGTG               CAC^GTG
BcgI          534  GCAAAGCTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI         156  GACGCC               G[GA]^CG[CT]C
BseAI        4736  TCCGGA               T^CCGGA
BsiWI        5412  CGTACG               C^GTACG
BspEI        4736  TCCGGA               T^CCGGA
BspHI        1295  TCATGA               T^CATGA
Bst1107I     2164  GTATAC               GTA^TAC
BstAPI       5142  GCAGCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      2164  GTATAC               GTA^TAC
DrdI          469  GACGGAGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1804  AGCGCT               AGC^GCT
EcoNI        2868  CCTCCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I         156  GACGCC               G[GA]^CG[CT]C
KpnI         1685  GGTACC               GGTAC^C
KspI         2686  CCGCGG               CCGC^GG
MroI         4736  TCCGGA               T^CCGGA
NaeI         2807  GCCGGC               GCC^GGC
NdeI         5307  CATATG               CA^TATG
NgoMIV       2807  GCCGGC               G^CCGGC
PaeR7I        397  CTCGAG               C^TCGAG
PinAI         884  ACCGGT               A^CCGGT
PmaCI        1959  CACGTG               CAC^GTG
PmlI         1959  CACGTG               CAC^GTG
RcaI         1295  TCATGA               T^CATGA
SacII        2686  CCGCGG               CCGC^GG
ScaI         6231  AGTACT               AGT^ACT
SexAI         821  ACCAGGT              A^CC[AT]GGT
StuI          139  AGGCCT               AGG^CCT
XbaI         3131  TCTAGA               T^CTAGA
XcmI         1864  CCAAAACCAATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          397  CTCGAG               C^TCGAG
XmnI         2672  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         2164  GTATAC               GT^[AC][GT]AC
             5661  GTCTAC              
AflIII       5768  ACACGT               A^C[GA][CT]GT
             6000  ACACGT              
AocI         4047  CCTGAGG              CC^T[ACGT]AGG
             4684  CCTCAGG             
AseI          873  ATTAAT               AT^TAAT
             6336  ATTAAT              
AsnI          873  ATTAAT               AT^TAAT
             6336  ATTAAT              
BciVI        2034  GGATAC               GTATCC(6/5)
             4728  GTATCC              
BlpI         2333  GCTGAGC              GC^T[ACGT]AGC
             2382  GCTCAGC             
Bpu1102I     2333  GCTGAGC              GC^T[ACGT]AGC
             2382  GCTCAGC             
BsaAI        1959  CACGTG               [CT]AC^GT[GA]
             6001  CACGTA              
BsiEI         319  CGGCCG               CG[GA][CT]^CG
              366  CGGCCG              
Bsp1407I     1519  TGTACA               T^GTACA
             2290  TGTACA              
BsrGI        1519  TGTACA               T^GTACA
             2290  TGTACA              
Bsu36I       4047  CCTGAGG              CC^T[ACGT]AGG
             4684  CCTCAGG             
CelII        2333  GCTGAGC              GC^T[ACGT]AGC
             2382  GCTCAGC             
DraIII        820  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2261  CACCATGTG           
EagI          319  CGGCCG               C^GGCCG
              366  CGGCCG              
EclXI         319  CGGCCG               C^GGCCG
              366  CGGCCG              
Eco52I        319  CGGCCG               C^GGCCG
              366  CGGCCG              
Eco81I       4047  CCTGAGG              CC^T[ACGT]AGG
             4684  CCTCAGG             
HincII       1905  GTCAAC               GT[CT]^[GA]AC
             3724  GTCAAC              
HindII       1905  GTCAAC               GT[CT]^[GA]AC
             3724  GTCAAC              
MfeI         1387  CAATTG               C^AATTG
             4993  CAATTG              
MunI         1387  CAATTG               C^AATTG
             4993  CAATTG              
NcoI          426  CCATGG               C^CATGG
             5742  CCATGG              
PflMI        2505  CCAACCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2630  CCAGTTTGTGG         
PshBI         873  ATTAAT               AT^TAAT
             6336  ATTAAT              
SphI         4509  GCATGC               GCATG^C
             5574  GCATGC              
SspBI        1519  TGTACA               T^GTACA
             2290  TGTACA              
Van91I       2505  CCAACCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2630  CCAGTTTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspDI       BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      ClaI        DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflFI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XhoII       
XmaCI       XmaI