##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

Acc65I      AhdI        AocI        AscI        Asp718I     
AspEI       BamHI       BbvCI       BlpI        Bpu1102I    
BsaBI       BsiWI       BsmBI       BssSI       BstBI       
Bsu36I      CelII       CpoI        DrdI        Eam1105I    
Eco81I      EcoNI       EcoRI       EcoRV       HpaI        
KpnI        MamI        MfeI        MluI        MunI        
NotI        NruI        NspV        PacI        PaeR7I      
PshAI       PvuI        RsrII       SbfI        SexAI       
SfuI        SnaBI       SpeI        Sse8387I    StuI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        2782  GACGTC               GACGT^C
AccIII         76  TCCGGA               T^CCGGA
AclI         4306  AACGTT               AA^CGTT
AflII        3184  CTTAAG               C^TTAAG
AgeI          365  ACCGGT               A^CCGGT
Asp700I      4334  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         550  TGCGCA               TGC^GCA
BcgI         1958  GCAGCCCTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4257  TGATCA               T^GATCA
BfrI         3184  CTTAAG               C^TTAAG
BseAI          76  TCCGGA               T^CCGGA
BsmI         3083  GAATGC               GAATGC(1/-1)
BspDI        1966  ATCGAT               AT^CGAT
BspEI          76  TCCGGA               T^CCGGA
BspHI        2414  TCATGA               T^CATGA
BsrBI        2467  GAGCGG               CCGCTC(-3/-3)
BsrDI        1103  GCAATG               GCAATG(2/0)
BssHII        461  GCGCGC               G^CGCGC
BstEII       1093  GGTCACC              G^GT[ACGT]ACC
BstPI        1093  GGTCACC              G^GT[ACGT]ACC
ClaI         1966  ATCGAT               AT^CGAT
EcoO65I      1093  GGTCACC              G^GT[ACGT]ACC
FbaI         4257  TGATCA               T^GATCA
FspI          550  TGCGCA               TGC^GCA
KspI          122  CCGCGG               CCGC^GG
MroI           76  TCCGGA               T^CCGGA
NdeI         4976  CATATG               CA^TATG
NheI         4375  GCTAGC               G^CTAGC
PinAI         365  ACCGGT               A^CCGGT
PmeI         3824  GTTTAAAC             GTTT^AAAC
Psp1406I     4306  AACGTT               AA^CGTT
RcaI         2414  TCATGA               T^CATGA
SacII         122  CCGCGG               CCGC^GG
SalI         2996  GTCGAC               G^TCGAC
SapI         3323  GAAGAGC              GCTCTTC(1/4)
SmaI         2029  CCCGGG               CCC^GGG
SphI         2059  GCATGC               GCATG^C
XmaCI        2029  CCCGGG               C^CCGGG
XmaI         2029  CCCGGG               C^CCGGG
XmnI         4334  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Aor51HI       881  AGCGCT               AGC^GCT
             2147  AGCGCT              
AseI         5657  ATTAAT               AT^TAAT
             5712  ATTAAT              
AsnI         5657  ATTAAT               AT^TAAT
             5712  ATTAAT              
AspI         1567  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2347  GACCATGTC           
AvaI           69  CTCGGG               C^[CT]CG[GA]G
             2029  CCCGGG              
BbrPI         787  CACGTG               CAC^GTG
             1626  CACGTG              
BglII         178  AGATCT               A^GATCT
             5616  AGATCT              
BsaAI         787  CACGTG               [CT]AC^GT[GA]
             1626  CACGTG              
BsaWI          76  TCCGGA               [AT]^CCGG[AT]
              365  ACCGGT              
BsoBI          69  CTCGGG               C^[CT]CG[GA]G
             2029  CCCGGG              
BspLU11I     3518  ACATGT               A^CATGT
             5581  ACATGT              
Bst1107I     4178  GTATAC               GTA^TAC
             5585  GTATAC              
BstZ17I      4178  GTATAC               GTA^TAC
             5585  GTATAC              
DraIII       2456  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2473  CACTCGGTG           
Eco47III      881  AGCGCT               AGC^GCT
             2147  AGCGCT              
FseI          100  GGCCGGCC             GGCCGG^CC
              116  GGCCGGCC            
HincII       2996  GTCGAC               GT[CT]^[GA]AC
             5198  GTCAAC              
HindII       2996  GTCGAC               GT[CT]^[GA]AC
             5198  GTCAAC              
HindIII      2919  AAGCTT               A^AGCTT
             3196  AAGCTT              
PflFI        1567  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2347  GACCATGTC           
PmaCI         787  CACGTG               CAC^GTG
             1626  CACGTG              
PmlI          787  CACGTG               CAC^GTG
             1626  CACGTG              
PshBI        5657  ATTAAT               AT^TAAT
             5712  ATTAAT              
ScaI         2850  AGTACT               AGT^ACT
             4120  AGTACT              
SfiI          988  GGCCTGCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1639  GGCCGCTGGGGCC       
SgrAI         534  CGCCGGCG             C[GA]^CCGG[CT]G
              564  CACCGGCG            
Tth111I      1567  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2347  GACCATGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       ApaI        ApaLI       ApoI        AspHI       
AvaII       AvrII       BalI        BanI        BanII       
BbeI        BbsI        BbvI        BciVI       BcnI        
BfaI        BglI        BlnI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    Bsp1407I    BspMI       
BsrFI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoRII      EcoT14I     EcoT22I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NsiI        NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII