##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
Acc65I AhdI AocI AscI Asp718I
AspEI BamHI BbvCI BlpI Bpu1102I
BsaBI BsiWI BsmBI BssSI BstBI
Bsu36I CelII CpoI DrdI Eam1105I
Eco81I EcoNI EcoRI EcoRV HpaI
KpnI MamI MfeI MluI MunI
NotI NruI NspV PacI PaeR7I
PshAI PvuI RsrII SbfI SexAI
SfuI SnaBI SpeI Sse8387I StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2782 GACGTC GACGT^C
AccIII 76 TCCGGA T^CCGGA
AclI 4306 AACGTT AA^CGTT
AflII 3184 CTTAAG C^TTAAG
AgeI 365 ACCGGT A^CCGGT
Asp700I 4334 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 550 TGCGCA TGC^GCA
BcgI 1958 GCAGCCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4257 TGATCA T^GATCA
BfrI 3184 CTTAAG C^TTAAG
BseAI 76 TCCGGA T^CCGGA
BsmI 3083 GAATGC GAATGC(1/-1)
BspDI 1966 ATCGAT AT^CGAT
BspEI 76 TCCGGA T^CCGGA
BspHI 2414 TCATGA T^CATGA
BsrBI 2467 GAGCGG CCGCTC(-3/-3)
BsrDI 1103 GCAATG GCAATG(2/0)
BssHII 461 GCGCGC G^CGCGC
BstEII 1093 GGTCACC G^GT[ACGT]ACC
BstPI 1093 GGTCACC G^GT[ACGT]ACC
ClaI 1966 ATCGAT AT^CGAT
EcoO65I 1093 GGTCACC G^GT[ACGT]ACC
FbaI 4257 TGATCA T^GATCA
FspI 550 TGCGCA TGC^GCA
KspI 122 CCGCGG CCGC^GG
MroI 76 TCCGGA T^CCGGA
NdeI 4976 CATATG CA^TATG
NheI 4375 GCTAGC G^CTAGC
PinAI 365 ACCGGT A^CCGGT
PmeI 3824 GTTTAAAC GTTT^AAAC
Psp1406I 4306 AACGTT AA^CGTT
RcaI 2414 TCATGA T^CATGA
SacII 122 CCGCGG CCGC^GG
SalI 2996 GTCGAC G^TCGAC
SapI 3323 GAAGAGC GCTCTTC(1/4)
SmaI 2029 CCCGGG CCC^GGG
SphI 2059 GCATGC GCATG^C
XmaCI 2029 CCCGGG C^CCGGG
XmaI 2029 CCCGGG C^CCGGG
XmnI 4334 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Aor51HI 881 AGCGCT AGC^GCT
2147 AGCGCT
AseI 5657 ATTAAT AT^TAAT
5712 ATTAAT
AsnI 5657 ATTAAT AT^TAAT
5712 ATTAAT
AspI 1567 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2347 GACCATGTC
AvaI 69 CTCGGG C^[CT]CG[GA]G
2029 CCCGGG
BbrPI 787 CACGTG CAC^GTG
1626 CACGTG
BglII 178 AGATCT A^GATCT
5616 AGATCT
BsaAI 787 CACGTG [CT]AC^GT[GA]
1626 CACGTG
BsaWI 76 TCCGGA [AT]^CCGG[AT]
365 ACCGGT
BsoBI 69 CTCGGG C^[CT]CG[GA]G
2029 CCCGGG
BspLU11I 3518 ACATGT A^CATGT
5581 ACATGT
Bst1107I 4178 GTATAC GTA^TAC
5585 GTATAC
BstZ17I 4178 GTATAC GTA^TAC
5585 GTATAC
DraIII 2456 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2473 CACTCGGTG
Eco47III 881 AGCGCT AGC^GCT
2147 AGCGCT
FseI 100 GGCCGGCC GGCCGG^CC
116 GGCCGGCC
HincII 2996 GTCGAC GT[CT]^[GA]AC
5198 GTCAAC
HindII 2996 GTCGAC GT[CT]^[GA]AC
5198 GTCAAC
HindIII 2919 AAGCTT A^AGCTT
3196 AAGCTT
PflFI 1567 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2347 GACCATGTC
PmaCI 787 CACGTG CAC^GTG
1626 CACGTG
PmlI 787 CACGTG CAC^GTG
1626 CACGTG
PshBI 5657 ATTAAT AT^TAAT
5712 ATTAAT
ScaI 2850 AGTACT AGT^ACT
4120 AGTACT
SfiI 988 GGCCTGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1639 GGCCGCTGGGGCC
SgrAI 534 CGCCGGCG C[GA]^CCGG[CT]G
564 CACCGGCG
Tth111I 1567 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2347 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspHI
AvaII AvrII BalI BanI BanII
BbeI BbsI BbvI BciVI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII