##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI BbeI BcgI BfrI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BsrFI Bst1107I BstBI BstZ17I
Cfr10I ClaI CpoI DrdI EcoRV
EcoT22I FseI KasI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SfoI SfuI
SgrAI SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 5044 GACGCC G[GA]^CG[CT]C
AhdI 3767 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2760 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1348 AGCGCT AGC^GCT
AseI 3857 ATTAAT AT^TAAT
AsnI 3857 ATTAAT AT^TAAT
AspEI 3767 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5126 TGCGCA TGC^GCA
AvrII 6412 CCTAGG C^CTAGG
BamHI 5853 GGATCC G^GATCC
BciVI 5754 GGATAC GTATCC(6/5)
BlnI 6412 CCTAGG C^CTAGG
BsaHI 5044 GACGCC G[GA]^CG[CT]C
BsiEI 268 CGGCCG CG[GA][CT]^CG
BspLU11I 4840 ACATGT A^CATGT
BsrBI 254 CCGCTC CCGCTC(-3/-3)
BssHII 4126 GCGCGC G^CGCGC
DraI 6023 TTTAAA TTT^AAA
EagI 268 CGGCCG C^GGCCG
Eam1105I 3767 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 268 CGGCCG C^GGCCG
Eco47III 1348 AGCGCT AGC^GCT
Eco52I 268 CGGCCG C^GGCCG
FspI 5126 TGCGCA TGC^GCA
Hin1I 5044 GACGCC G[GA]^CG[CT]C
HpaI 4330 GTTAAC GTT^AAC
KspI 552 CCGCGG CCGC^GG
NdeI 2549 CATATG CA^TATG
NheI 1886 GCTAGC G^CTAGC
PshBI 3857 ATTAAT AT^TAAT
SacII 552 CCGCGG CCGC^GG
SbfI 3287 CCTGCAGG CCTGCA^GG
SexAI 3223 ACCTGGT A^CC[AT]GGT
SfiI 5911 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 6191 GCATGC GCATG^C
Sse8387I 3287 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3360 GGTACC G^GTACC
5810 GGTACC
Asp718I 3360 GGTACC G^GTACC
5810 GGTACC
BbvCI 3194 CCTCAGC CCTCAGC(-5/-2)
3159 GCTGAGG
BclI 1766 TGATCA T^GATCA
5051 TGATCA
BglI 322 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5912 GCCACCCTGGC
BlpI 998 GCTAAGC GC^T[ACGT]AGC
1374 GCTAAGC
Bpu1102I 998 GCTAAGC GC^T[ACGT]AGC
1374 GCTAAGC
BsmBI 16 GAGACG CGTCTC(1/5)
4569 GAGACG
BsmI 2108 GCATTC GAATGC(1/-1)
3791 GCATTC
Bsp1407I 3030 TGTACA T^GTACA
5800 TGTACA
BsrGI 3030 TGTACA T^GTACA
5800 TGTACA
BssSI 3302 CACGAG CACGAG(-5/-1)
2878 CTCGTG
BstEII 3293 GGTCACC G^GT[ACGT]ACC
5785 GGTGACC
BstPI 3293 GGTCACC G^GT[ACGT]ACC
5785 GGTGACC
CelII 998 GCTAAGC GC^T[ACGT]AGC
1374 GCTAAGC
EcoO65I 3293 GGTCACC G^GT[ACGT]ACC
5785 GGTGACC
FbaI 1766 TGATCA T^GATCA
5051 TGATCA
HaeII 1348 AGCGCT [GA]GCGC^[CT]
5869 AGCGCC
HgaI 1787 GACGC GACGC(5/10)
5044 GACGC
HincII 4330 GTTAAC GT[CT]^[GA]AC
4372 GTCAAC
HindII 4330 GTTAAC GT[CT]^[GA]AC
4372 GTCAAC
HindIII 771 AAGCTT A^AGCTT
989 AAGCTT
KpnI 3360 GGTACC GGTAC^C
5810 GGTACC
SmaI 1451 CCCGGG CCC^GGG
4580 CCCGGG
SspBI 3030 TGTACA T^GTACA
5800 TGTACA
XbaI 1541 TCTAGA T^CTAGA
4186 TCTAGA
XmaCI 1451 CCCGGG C^CCGGG
4580 CCCGGG
XmaI 1451 CCCGGG C^CCGGG
4580 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AocI
ApaI ApaLI ApoI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI