##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AscI        
BamHI       BciVI       BclI        BfrI        BglII       
BlpI        Bpu1102I    BsaBI       BseAI       BsiWI       
BspEI       BspHI       BspLU11I    Bst1107I    BstBI       
BstZ17I     CelII       EcoRI       FbaI        FseI        
HindIII     HpaI        MamI        MluI        MroI        
NaeI        NdeI        NgoMIV      NotI        NspV        
PacI        PaeR7I      PmeI        PshAI       Psp1406I    
RcaI        SalI        SbfI        ScaI        SfuI        
SgrAI       SnaBI       Sse8387I    SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       4785  GGTACC               G^GTACC
AgeI         1280  ACCGGT               A^CCGGT
AocI         2789  CCTCAGG              CC^T[ACGT]AGG
AseI         3437  ATTAAT               AT^TAAT
AsnI         3437  ATTAAT               AT^TAAT
Asp718I      4785  GGTACC               G^GTACC
AviII        4136  TGCGCA               TGC^GCA
BbrPI        4932  CACGTG               CAC^GTG
BbvCI        2672  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1257  CGACGCATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BspDI         328  ATCGAT               AT^CGAT
BsrBI         414  GAGCGG               CCGCTC(-3/-3)
BsrDI        5571  CATTGC               GCAATG(2/0)
BssHII        230  GCGCGC               G^CGCGC
Bsu36I       2789  CCTCAGG              CC^T[ACGT]AGG
ClaI          328  ATCGAT               AT^CGAT
CpoI         1199  CGGACCG              CG^G[AT]CCG
DraIII       5280  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1728  GACCCAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2789  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1725  CCTGACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        4098  GATATC               GAT^ATC
EcoT22I      1464  ATGCAT               ATGCA^T
FspI         4136  TGCGCA               TGC^GCA
KpnI         4785  GGTACC               GGTAC^C
MfeI         4034  CAATTG               C^AATTG
MunI         4034  CAATTG               C^AATTG
NheI          684  GCTAGC               G^CTAGC
NruI         5483  TCGCGA               TCG^CGA
NsiI         1464  ATGCAT               ATGCA^T
PflMI        3258  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1280  ACCGGT               A^CCGGT
PmaCI        4932  CACGTG               CAC^GTG
PmlI         4932  CACGTG               CAC^GTG
PshBI        3437  ATTAAT               AT^TAAT
PvuI           24  CGATCG               CGAT^CG
RsrII        1199  CGGACCG              CG^G[AT]CCG
SapI         3506  GAAGAGC              GCTCTTC(1/4)
SexAI         755  ACCTGGT              A^CC[AT]GGT
SfiI         1005  GGCCGAGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         1879  ACTAGT               A^CTAGT
SphI         3489  GCATGC               GCATG^C
SspI         1982  AATATT               AAT^ATT
Van91I       3258  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AatII        1051  GACGTC               GACGT^C
             3552  GACGTC              
AcsI         2013  GAATTT               [GA]^AATT[CT]
             4216  AAATTC              
AflIII       4933  ACGTGT               A^C[GA][CT]GT
             5387  ACGTGT              
AhdI         1279  GACCGGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3891  GACCTGTCGTC         
Alw44I       4929  GTGCAC               G^TGCAC
             5277  GTGCAC              
Aor51HI       731  AGCGCT               AGC^GCT
              870  AGCGCT              
ApaLI        4929  GTGCAC               G^TGCAC
             5277  GTGCAC              
ApoI         2013  GAATTT               [GA]^AATT[CT]
             4216  AAATTC              
AspEI        1279  GACCGGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3891  GACCTGTCGTC         
AvrII        3885  CCTAGG               C^CTAGG
             3910  CCTAGG              
BbeI          115  GGCGCC               GGCGC^C
              920  GGCGCC              
BlnI         3885  CCTAGG               C^CTAGG
             3910  CCTAGG              
BsmBI        1087  GAGACG               CGTCTC(1/5)
             2106  GAGACG              
BsmI         1462  GAATGC               GAATGC(1/-1)
             1065  GCATTC              
BstEII        360  GGTGACC              G^GT[ACGT]ACC
             3528  GGTAACC             
BstPI         360  GGTGACC              G^GT[ACGT]ACC
             3528  GGTAACC             
DraI         5543  TTTAAA               TTT^AAA
             5562  TTTAAA              
EagI          225  CGGCCG               C^GGCCG
             1589  CGGCCG              
Eam1105I     1279  GACCGGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3891  GACCTGTCGTC         
EclXI         225  CGGCCG               C^GGCCG
             1589  CGGCCG              
Eco47III      731  AGCGCT               AGC^GCT
              870  AGCGCT              
Eco52I        225  CGGCCG               C^GGCCG
             1589  CGGCCG              
EcoO65I       360  GGTGACC              G^GT[ACGT]ACC
             3528  GGTAACC             
KasI          115  GGCGCC               G^GCGCC
              920  GGCGCC              
NarI          115  GGCGCC               GG^CGCC
              920  GGCGCC              
NcoI         1016  CCATGG               C^CATGG
             3124  CCATGG              
NspI         2277  GCATGT               [GA]CATG^[CT]
             3489  GCATGC              
SfoI          115  GGCGCC               GGC^GCC
              920  GGCGCC              
StuI         2524  AGGCCT               AGG^CCT
             3189  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrFI       BsrGI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflFI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        SspBI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XcmI        
XhoII       XmaCI       XmaI        XmnI