################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AccI AccIII AclI AflII AscI BamHI BciVI BclI BfrI BglII BlpI Bpu1102I BsaBI BseAI BsiWI BspEI BspHI BspLU11I Bst1107I BstBI BstZ17I CelII EcoRI FbaI FseI HindIII HpaI MamI MluI MroI NaeI NdeI NgoMIV NotI NspV PacI PaeR7I PmeI PshAI Psp1406I RcaI SalI SbfI ScaI SfuI SgrAI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 4785 GGTACC G^GTACC AgeI 1280 ACCGGT A^CCGGT AocI 2789 CCTCAGG CC^T[ACGT]AGG AseI 3437 ATTAAT AT^TAAT AsnI 3437 ATTAAT AT^TAAT Asp718I 4785 GGTACC G^GTACC AviII 4136 TGCGCA TGC^GCA BbrPI 4932 CACGTG CAC^GTG BbvCI 2672 CCTCAGC CCTCAGC(-5/-2) BcgI 1257 CGACGCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BspDI 328 ATCGAT AT^CGAT BsrBI 414 GAGCGG CCGCTC(-3/-3) BsrDI 5571 CATTGC GCAATG(2/0) BssHII 230 GCGCGC G^CGCGC Bsu36I 2789 CCTCAGG CC^T[ACGT]AGG ClaI 328 ATCGAT AT^CGAT CpoI 1199 CGGACCG CG^G[AT]CCG DraIII 5280 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1728 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2789 CCTCAGG CC^T[ACGT]AGG EcoNI 1725 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 4098 GATATC GAT^ATC EcoT22I 1464 ATGCAT ATGCA^T FspI 4136 TGCGCA TGC^GCA KpnI 4785 GGTACC GGTAC^C MfeI 4034 CAATTG C^AATTG MunI 4034 CAATTG C^AATTG NheI 684 GCTAGC G^CTAGC NruI 5483 TCGCGA TCG^CGA NsiI 1464 ATGCAT ATGCA^T PflMI 3258 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1280 ACCGGT A^CCGGT PmaCI 4932 CACGTG CAC^GTG PmlI 4932 CACGTG CAC^GTG PshBI 3437 ATTAAT AT^TAAT PvuI 24 CGATCG CGAT^CG RsrII 1199 CGGACCG CG^G[AT]CCG SapI 3506 GAAGAGC GCTCTTC(1/4) SexAI 755 ACCTGGT A^CC[AT]GGT SfiI 1005 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 1879 ACTAGT A^CTAGT SphI 3489 GCATGC GCATG^C SspI 1982 AATATT AAT^ATT Van91I 3258 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AatII 1051 GACGTC GACGT^C 3552 GACGTC AcsI 2013 GAATTT [GA]^AATT[CT] 4216 AAATTC AflIII 4933 ACGTGT A^C[GA][CT]GT 5387 ACGTGT AhdI 1279 GACCGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3891 GACCTGTCGTC Alw44I 4929 GTGCAC G^TGCAC 5277 GTGCAC Aor51HI 731 AGCGCT AGC^GCT 870 AGCGCT ApaLI 4929 GTGCAC G^TGCAC 5277 GTGCAC ApoI 2013 GAATTT [GA]^AATT[CT] 4216 AAATTC AspEI 1279 GACCGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3891 GACCTGTCGTC AvrII 3885 CCTAGG C^CTAGG 3910 CCTAGG BbeI 115 GGCGCC GGCGC^C 920 GGCGCC BlnI 3885 CCTAGG C^CTAGG 3910 CCTAGG BsmBI 1087 GAGACG CGTCTC(1/5) 2106 GAGACG BsmI 1462 GAATGC GAATGC(1/-1) 1065 GCATTC BstEII 360 GGTGACC G^GT[ACGT]ACC 3528 GGTAACC BstPI 360 GGTGACC G^GT[ACGT]ACC 3528 GGTAACC DraI 5543 TTTAAA TTT^AAA 5562 TTTAAA EagI 225 CGGCCG C^GGCCG 1589 CGGCCG Eam1105I 1279 GACCGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3891 GACCTGTCGTC EclXI 225 CGGCCG C^GGCCG 1589 CGGCCG Eco47III 731 AGCGCT AGC^GCT 870 AGCGCT Eco52I 225 CGGCCG C^GGCCG 1589 CGGCCG EcoO65I 360 GGTGACC G^GT[ACGT]ACC 3528 GGTAACC KasI 115 GGCGCC G^GCGCC 920 GGCGCC NarI 115 GGCGCC GG^CGCC 920 GGCGCC NcoI 1016 CCATGG C^CATGG 3124 CCATGG NspI 2277 GCATGT [GA]CATG^[CT] 3489 GCATGC SfoI 115 GGCGCC GGC^GCC 920 GGCGCC StuI 2524 AGGCCT AGG^CCT 3189 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmaCI XmaI XmnI