################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AclI AflIII AgeI AhdI AocI Aor51HI AscI Asp718I AspEI BclI BglII BsaBI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HindIII KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI PacI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SexAI SfiI SgrAI SnaBI SpeI SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 3089 GTCTAC GT^[AC][GT]AC AcyI 93 GGCGCC G[GA]^CG[CT]C AflII 936 CTTAAG C^TTAAG Alw44I 1880 GTGCAC G^TGCAC ApaI 593 GGGCCC GGGCC^C ApaLI 1880 GTGCAC G^TGCAC AseI 3012 ATTAAT AT^TAAT AsnI 3012 ATTAAT AT^TAAT AspI 3642 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3571 CCTAGG C^CTAGG BalI 3457 TGGCCA TGG^CCA BamHI 1622 GGATCC G^GATCC BbeI 93 GGCGCC GGCGC^C BbrPI 129 CACGTG CAC^GTG BfrI 936 CTTAAG C^TTAAG BlnI 3571 CCTAGG C^CTAGG BlpI 653 GCTGAGC GC^T[ACGT]AGC Bpu1102I 653 GCTGAGC GC^T[ACGT]AGC BsaAI 129 CACGTG [CT]AC^GT[GA] BsaHI 93 GGCGCC G[GA]^CG[CT]C BsmBI 408 GAGACG CGTCTC(1/5) BssHII 2489 GCGCGC G^CGCGC BstBI 3601 TTCGAA TT^CGAA CelII 653 GCTGAGC GC^T[ACGT]AGC DraI 3256 TTTAAA TTT^AAA DraIII 1341 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG Hin1I 93 GGCGCC G[GA]^CG[CT]C HincII 3347 GTTAAC GT[CT]^[GA]AC HindII 3347 GTTAAC GT[CT]^[GA]AC HpaI 3347 GTTAAC GTT^AAC KasI 93 GGCGCC G^GCGCC MluNI 3457 TGGCCA TGG^CCA MscI 3457 TGGCCA TGG^CCA NarI 93 GGCGCC GG^CGCC NcoI 2626 CCATGG C^CATGG NspI 2029 GCATGC [GA]CATG^[CT] NspV 3601 TTCGAA TT^CGAA PaeR7I 2001 CTCGAG C^TCGAG PflFI 3642 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 129 CACGTG CAC^GTG PmlI 129 CACGTG CAC^GTG PshBI 3012 ATTAAT AT^TAAT ScaI 3193 AGTACT AGT^ACT SfoI 93 GGCGCC GGC^GCC SfuI 3601 TTCGAA TT^CGAA SphI 2029 GCATGC GCATG^C StuI 2140 AGGCCT AGG^CCT Tth111I 3642 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1805 TCTAGA T^CTAGA XcmI 469 CCAGGGAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2001 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 832 TCCGGA T^CCGGA 2703 TCCGGA Asp700I 309 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1248 GAAGACCTTC AviII 297 TGCGCA TGC^GCA 2026 TGCGCA BanI 93 GGCGCC G^G[CT][GA]CC 2263 GGCACC BcgI 142 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1028 GCAGGAGTGTCG BciVI 3221 GGATAC GTATCC(6/5) 3235 GGATAC BseAI 832 TCCGGA T^CCGGA 2703 TCCGGA BspEI 832 TCCGGA T^CCGGA 2703 TCCGGA BspMI 1903 ACCTGC ACCTGC(4/8) 2542 GCAGGT BssSI 528 CACGAG CACGAG(-5/-1) 395 CTCGTG BstYI 1622 GGATCC [GA]^GATC[CT] 2107 AGATCC EcoRI 956 GAATTC G^AATTC 1499 GAATTC FspI 297 TGCGCA TGC^GCA 2026 TGCGCA MaeII 130 ACGT A^CGT 2932 ACGT MflI 1622 GGATCC [GA]^GATC[CT] 2107 AGATCC MroI 832 TCCGGA T^CCGGA 2703 TCCGGA PflMI 329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2138 CCAGGCCTTGG SmaI 2384 CCCGGG CCC^GGG 2888 CCCGGG Van91I 329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2138 CCAGGCCTTGG XhoII 1622 GGATCC [GA]^GATC[CT] 2107 AGATCC XmaCI 2384 CCCGGG C^CCGGG 2888 CCCGGG XmaI 2384 CCCGGG C^CCGGG 2888 CCCGGG XmnI 309 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1248 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I