##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AgeI        
AhdI        AocI        Aor51HI     AscI        Asp718I     
AspEI       BclI        BglII       BsaBI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       Bst1107I    BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        HindIII     KpnI        KspI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SexAI       SfiI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         3089  GTCTAC               GT^[AC][GT]AC
AcyI           93  GGCGCC               G[GA]^CG[CT]C
AflII         936  CTTAAG               C^TTAAG
Alw44I       1880  GTGCAC               G^TGCAC
ApaI          593  GGGCCC               GGGCC^C
ApaLI        1880  GTGCAC               G^TGCAC
AseI         3012  ATTAAT               AT^TAAT
AsnI         3012  ATTAAT               AT^TAAT
AspI         3642  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3571  CCTAGG               C^CTAGG
BalI         3457  TGGCCA               TGG^CCA
BamHI        1622  GGATCC               G^GATCC
BbeI           93  GGCGCC               GGCGC^C
BbrPI         129  CACGTG               CAC^GTG
BfrI          936  CTTAAG               C^TTAAG
BlnI         3571  CCTAGG               C^CTAGG
BlpI          653  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      653  GCTGAGC              GC^T[ACGT]AGC
BsaAI         129  CACGTG               [CT]AC^GT[GA]
BsaHI          93  GGCGCC               G[GA]^CG[CT]C
BsmBI         408  GAGACG               CGTCTC(1/5)
BssHII       2489  GCGCGC               G^CGCGC
BstBI        3601  TTCGAA               TT^CGAA
CelII         653  GCTGAGC              GC^T[ACGT]AGC
DraI         3256  TTTAAA               TTT^AAA
DraIII       1341  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Hin1I          93  GGCGCC               G[GA]^CG[CT]C
HincII       3347  GTTAAC               GT[CT]^[GA]AC
HindII       3347  GTTAAC               GT[CT]^[GA]AC
HpaI         3347  GTTAAC               GTT^AAC
KasI           93  GGCGCC               G^GCGCC
MluNI        3457  TGGCCA               TGG^CCA
MscI         3457  TGGCCA               TGG^CCA
NarI           93  GGCGCC               GG^CGCC
NcoI         2626  CCATGG               C^CATGG
NspI         2029  GCATGC               [GA]CATG^[CT]
NspV         3601  TTCGAA               TT^CGAA
PaeR7I       2001  CTCGAG               C^TCGAG
PflFI        3642  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         129  CACGTG               CAC^GTG
PmlI          129  CACGTG               CAC^GTG
PshBI        3012  ATTAAT               AT^TAAT
ScaI         3193  AGTACT               AGT^ACT
SfoI           93  GGCGCC               GGC^GCC
SfuI         3601  TTCGAA               TT^CGAA
SphI         2029  GCATGC               GCATG^C
StuI         2140  AGGCCT               AGG^CCT
Tth111I      3642  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1805  TCTAGA               T^CTAGA
XcmI          469  CCAGGGAAATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2001  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccIII        832  TCCGGA               T^CCGGA
             2703  TCCGGA              
Asp700I       309  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1248  GAAGACCTTC          
AviII         297  TGCGCA               TGC^GCA
             2026  TGCGCA              
BanI           93  GGCGCC               G^G[CT][GA]CC
             2263  GGCACC              
BcgI          142  GCAAGAAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1028  GCAGGAGTGTCG        
BciVI        3221  GGATAC               GTATCC(6/5)
             3235  GGATAC              
BseAI         832  TCCGGA               T^CCGGA
             2703  TCCGGA              
BspEI         832  TCCGGA               T^CCGGA
             2703  TCCGGA              
BspMI        1903  ACCTGC               ACCTGC(4/8)
             2542  GCAGGT              
BssSI         528  CACGAG               CACGAG(-5/-1)
              395  CTCGTG              
BstYI        1622  GGATCC               [GA]^GATC[CT]
             2107  AGATCC              
EcoRI         956  GAATTC               G^AATTC
             1499  GAATTC              
FspI          297  TGCGCA               TGC^GCA
             2026  TGCGCA              
MaeII         130  ACGT                 A^CGT
             2932  ACGT                
MflI         1622  GGATCC               [GA]^GATC[CT]
             2107  AGATCC              
MroI          832  TCCGGA               T^CCGGA
             2703  TCCGGA              
PflMI         329  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2138  CCAGGCCTTGG         
SmaI         2384  CCCGGG               CCC^GGG
             2888  CCCGGG              
Van91I        329  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2138  CCAGGCCTTGG         
XhoII        1622  GGATCC               [GA]^GATC[CT]
             2107  AGATCC              
XmaCI        2384  CCCGGG               C^CCGGG
             2888  CCCGGG              
XmaI         2384  CCCGGG               C^CCGGG
             2888  CCCGGG              
XmnI          309  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1248  GAAGACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      SbfI        ScrFI       SfaNI       SfcI        
SmlI        Sse8387I    StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I