##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

Acc65I      AclI        AgeI        AscI        AseI        
AsnI        Asp718I     BbrPI       BciVI       BclI        
BsiEI       BsiWI       BssHII      Bst1107I    BstBI       
BstZ17I     DraI        EagI        EclXI       Eco52I      
EcoRI       EcoT22I     FbaI        HpaI        KpnI        
MfeI        MluI        MunI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        SalI        SbfI        SfuI        SgrAI       
SnaBI       Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        3752  GACGTC               GACGT^C
AcsI         1712  GAATTT               [GA]^AATT[CT]
AflIII       4486  ACATGT               A^C[GA][CT]GT
AhdI         1593  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         1712  GAATTT               [GA]^AATT[CT]
AspEI        1593  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2832  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        8148  CCTAGG               C^CTAGG
BamHI         433  GGATCC               G^GATCC
BlnI         8148  CCTAGG               C^CTAGG
BsaAI        1221  TACGTG               [CT]AC^GT[GA]
BsaBI        8223  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI        1515  ATCGAT               AT^CGAT
BspLU11I     4486  ACATGT               A^CATGT
ClaI         1515  ATCGAT               AT^CGAT
CpoI         4444  CGGTCCG              CG^G[AT]CCG
Eam1105I     1593  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1518  GATATC               GAT^ATC
FseI         5228  GGCCGGCC             GGCCGG^CC
KspI          161  CCGCGG               CCGC^GG
MamI         8223  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI         1229  GCTAGC               G^CTAGC
PflFI        2832  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         852  GACCATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        4444  CGGTCCG              CG^G[AT]CCG
SacII         161  CCGCGG               CCGC^GG
SapI         7770  GAAGAGC              GCTCTTC(1/4)
ScaI         5536  AGTACT               AGT^ACT
SexAI        2833  ACCTGGT              A^CC[AT]GGT
SfiI         6017  GGCCTCGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI          706  AATATT               AAT^ATT
Tth111I      2832  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3506  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII         25  TCCGGA               T^CCGGA
             3430  TCCGGA              
Alw44I       4385  GTGCAC               G^TGCAC
             7399  GTGCAC              
Aor51HI      3270  AGCGCT               AGC^GCT
             6276  AGCGCT              
ApaLI        4385  GTGCAC               G^TGCAC
             7399  GTGCAC              
Asp700I      1115  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             7848  GAAATGCTTC          
BbeI          153  GGCGCC               GGCGC^C
             6132  GGCGCC              
BcgI         4640  CGAGGCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4100  GCAGGCCTCTCG        
BsaWI          25  TCCGGA               [AT]^CCGG[AT]
             3430  TCCGGA              
BseAI          25  TCCGGA               T^CCGGA
             3430  TCCGGA              
BsmI         3047  GAATGC               GAATGC(1/-1)
             5077  GCATTC              
BspEI          25  TCCGGA               T^CCGGA
             3430  TCCGGA              
BspHI         664  TCATGA               T^CATGA
              698  TCATGA              
BssSI         891  CACGAG               CACGAG(-5/-1)
              985  CACGAG              
Eco47III     3270  AGCGCT               AGC^GCT
             6276  AGCGCT              
HincII       1613  GTCAAC               GT[CT]^[GA]AC
             7133  GTTGAC              
HindII       1613  GTCAAC               GT[CT]^[GA]AC
             7133  GTTGAC              
KasI          153  GGCGCC               G^GCGCC
             6132  GGCGCC              
MroI           25  TCCGGA               T^CCGGA
             3430  TCCGGA              
NarI          153  GGCGCC               GG^CGCC
             6132  GGCGCC              
PaeR7I       3541  CTCGAG               C^TCGAG
             4132  CTCGAG              
RcaI          664  TCATGA               T^CATGA
              698  TCATGA              
SfoI          153  GGCGCC               GGC^GCC
             6132  GGCGCC              
SpeI         2474  ACTAGT               A^CTAGT
             7436  ACTAGT              
XhoI         3541  CTCGAG               C^TCGAG
             4132  CTCGAG              
XmnI         1115  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             7848  GAAATGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  152 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflII       AluI        AlwI        AlwNI       AocI        
ApaI        AspHI       AvaI        AvaII       AviII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BfrI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SphI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI