##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
Acc65I AclI AgeI AscI AseI
AsnI Asp718I BbrPI BciVI BclI
BsiEI BsiWI BssHII Bst1107I BstBI
BstZ17I DraI EagI EclXI Eco52I
EcoRI EcoT22I FbaI HpaI KpnI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SalI SbfI SfuI SgrAI
SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3752 GACGTC GACGT^C
AcsI 1712 GAATTT [GA]^AATT[CT]
AflIII 4486 ACATGT A^C[GA][CT]GT
AhdI 1593 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1712 GAATTT [GA]^AATT[CT]
AspEI 1593 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2832 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 8148 CCTAGG C^CTAGG
BamHI 433 GGATCC G^GATCC
BlnI 8148 CCTAGG C^CTAGG
BsaAI 1221 TACGTG [CT]AC^GT[GA]
BsaBI 8223 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1515 ATCGAT AT^CGAT
BspLU11I 4486 ACATGT A^CATGT
ClaI 1515 ATCGAT AT^CGAT
CpoI 4444 CGGTCCG CG^G[AT]CCG
Eam1105I 1593 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1518 GATATC GAT^ATC
FseI 5228 GGCCGGCC GGCCGG^CC
KspI 161 CCGCGG CCGC^GG
MamI 8223 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1229 GCTAGC G^CTAGC
PflFI 2832 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 852 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4444 CGGTCCG CG^G[AT]CCG
SacII 161 CCGCGG CCGC^GG
SapI 7770 GAAGAGC GCTCTTC(1/4)
ScaI 5536 AGTACT AGT^ACT
SexAI 2833 ACCTGGT A^CC[AT]GGT
SfiI 6017 GGCCTCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 706 AATATT AAT^ATT
Tth111I 2832 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3506 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 25 TCCGGA T^CCGGA
3430 TCCGGA
Alw44I 4385 GTGCAC G^TGCAC
7399 GTGCAC
Aor51HI 3270 AGCGCT AGC^GCT
6276 AGCGCT
ApaLI 4385 GTGCAC G^TGCAC
7399 GTGCAC
Asp700I 1115 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7848 GAAATGCTTC
BbeI 153 GGCGCC GGCGC^C
6132 GGCGCC
BcgI 4640 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4100 GCAGGCCTCTCG
BsaWI 25 TCCGGA [AT]^CCGG[AT]
3430 TCCGGA
BseAI 25 TCCGGA T^CCGGA
3430 TCCGGA
BsmI 3047 GAATGC GAATGC(1/-1)
5077 GCATTC
BspEI 25 TCCGGA T^CCGGA
3430 TCCGGA
BspHI 664 TCATGA T^CATGA
698 TCATGA
BssSI 891 CACGAG CACGAG(-5/-1)
985 CACGAG
Eco47III 3270 AGCGCT AGC^GCT
6276 AGCGCT
HincII 1613 GTCAAC GT[CT]^[GA]AC
7133 GTTGAC
HindII 1613 GTCAAC GT[CT]^[GA]AC
7133 GTTGAC
KasI 153 GGCGCC G^GCGCC
6132 GGCGCC
MroI 25 TCCGGA T^CCGGA
3430 TCCGGA
NarI 153 GGCGCC GG^CGCC
6132 GGCGCC
PaeR7I 3541 CTCGAG C^TCGAG
4132 CTCGAG
RcaI 664 TCATGA T^CATGA
698 TCATGA
SfoI 153 GGCGCC GGC^GCC
6132 GGCGCC
SpeI 2474 ACTAGT A^CTAGT
7436 ACTAGT
XhoI 3541 CTCGAG C^TCGAG
4132 CTCGAG
XmnI 1115 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7848 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI