##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AscI AviII BamHI BbeI BciVI
BclI BglI BseAI BsiWI BspEI
BspLU11I BsrDI BsrFI BssHII Bst1107I
BstUI BstZ17I Cfr10I CpoI DraIII
EagI EclXI Eco52I FbaI FseI
FspI KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SfiI SfoI SgrAI
SnaBI SphI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 476 GGTACC G^GTACC
AcyI 1230 GGCGTC G[GA]^CG[CT]C
AflII 5257 CTTAAG C^TTAAG
AflIII 1399 ACACGT A^C[GA][CT]GT
AhdI 186 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 5272 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 476 GGTACC G^GTACC
AspEI 186 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 891 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5044 TGGCCA TGG^CCA
BcgI 1052 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5257 CTTAAG C^TTAAG
BsaBI 2765 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1230 GGCGTC G[GA]^CG[CT]C
BsaWI 3918 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2661 TGTACA T^GTACA
BspDI 674 ATCGAT AT^CGAT
BspHI 2247 TCATGA T^CATGA
BsrBI 3217 CCGCTC CCGCTC(-3/-3)
BsrGI 2661 TGTACA T^GTACA
BstBI 3727 TTCGAA TT^CGAA
BstEII 1528 GGTAACC G^GT[ACGT]ACC
BstPI 1528 GGTAACC G^GT[ACGT]ACC
ClaI 674 ATCGAT AT^CGAT
Eam1105I 186 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1528 GGTAACC G^GT[ACGT]ACC
EcoRI 4129 GAATTC G^AATTC
Hin1I 1230 GGCGTC G[GA]^CG[CT]C
HindIII 2785 AAGCTT A^AGCTT
HpaI 1793 GTTAAC GTT^AAC
KpnI 476 GGTACC GGTAC^C
MamI 2765 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5044 TGGCCA TGG^CCA
MscI 5044 TGGCCA TGG^CCA
NdeI 1915 CATATG CA^TATG
NspV 3727 TTCGAA TT^CGAA
PflFI 891 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2247 TCATGA T^CATGA
SalI 4806 GTCGAC G^TCGAC
SapI 5027 GAAGAGC GCTCTTC(1/4)
ScaI 2419 AGTACT AGT^ACT
SfuI 3727 TTCGAA TT^CGAA
SmaI 930 CCCGGG CCC^GGG
SpeI 269 ACTAGT A^CTAGT
SspBI 2661 TGTACA T^GTACA
StuI 4422 AGGCCT AGG^CCT
SwaI 414 ATTTAAAT ATTT^AAAT
Tth111I 891 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 930 CCCGGG C^CCGGG
XmaI 930 CCCGGG C^CCGGG
XmnI 5272 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AocI 2856 CCTTAGG CC^T[ACGT]AGG
4274 CCTGAGG
Aor51HI 1701 AGCGCT AGC^GCT
2053 AGCGCT
ApaI 1989 GGGCCC GGGCC^C
2076 GGGCCC
AseI 2565 ATTAAT AT^TAAT
3766 ATTAAT
AsnI 2565 ATTAAT AT^TAAT
3766 ATTAAT
AvrII 2928 CCTAGG C^CTAGG
3641 CCTAGG
BbrPI 1444 CACGTG CAC^GTG
3942 CACGTG
BlnI 2928 CCTAGG C^CTAGG
3641 CCTAGG
BlpI 1555 GCTGAGC GC^T[ACGT]AGC
3897 GCTCAGC
Bpu1102I 1555 GCTGAGC GC^T[ACGT]AGC
3897 GCTCAGC
BsmBI 1672 CGTCTC CGTCTC(1/5)
4779 GAGACG
BsmI 4337 GAATGC GAATGC(1/-1)
4593 GAATGC
BssSI 699 CACGAG CACGAG(-5/-1)
2963 CACGAG
BstAPI 2206 GCACGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3980 GCAGGGTGTGC
BstXI 2437 CCAATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4787 CCAGTCTTTTGG
Bsu36I 2856 CCTTAGG CC^T[ACGT]AGG
4274 CCTGAGG
CelII 1555 GCTGAGC GC^T[ACGT]AGC
3897 GCTCAGC
DrdI 4781 GACGCTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5229 GACTGGCGAGTC
Eco47III 1701 AGCGCT AGC^GCT
2053 AGCGCT
Eco81I 2856 CCTTAGG CC^T[ACGT]AGG
4274 CCTGAGG
EcoNI 3204 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3666 CCTGGGGAAGG
EcoRV 2406 GATATC GAT^ATC
4260 GATATC
NcoI 1411 CCATGG C^CATGG
4490 CCATGG
NspI 1754 GCATGT [GA]CATG^[CT]
3121 ACATGC
PaeR7I 1768 CTCGAG C^TCGAG
3690 CTCGAG
PflMI 2018 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2192 CCATTCCTTGG
PmaCI 1444 CACGTG CAC^GTG
3942 CACGTG
PmlI 1444 CACGTG CAC^GTG
3942 CACGTG
PshBI 2565 ATTAAT AT^TAAT
3766 ATTAAT
PvuII 481 CAGCTG CAG^CTG
3126 CAGCTG
SacI 555 GAGCTC GAGCT^C
4116 GAGCTC
SbfI 599 CCTGCAGG CCTGCA^GG
4196 CCTGCAGG
SexAI 1339 ACCAGGT A^CC[AT]GGT
2097 ACCTGGT
Sse8387I 599 CCTGCAGG CCTGCA^GG
4196 CCTGCAGG
Van91I 2018 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2192 CCATTCCTTGG
XhoI 1768 CTCGAG C^TCGAG
3690 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII