##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AgeI        
AscI        AviII       BamHI       BbeI        BciVI       
BclI        BglI        BseAI       BsiWI       BspEI       
BspLU11I    BsrDI       BsrFI       BssHII      Bst1107I    
BstUI       BstZ17I     Cfr10I      CpoI        DraIII      
EagI        EclXI       Eco52I      FbaI        FseI        
FspI        KasI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SfiI        SfoI        SgrAI       
SnaBI       SphI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        476  GGTACC               G^GTACC
AcyI         1230  GGCGTC               G[GA]^CG[CT]C
AflII        5257  CTTAAG               C^TTAAG
AflIII       1399  ACACGT               A^C[GA][CT]GT
AhdI          186  GACATTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      5272  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       476  GGTACC               G^GTACC
AspEI         186  GACATTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          891  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         5044  TGGCCA               TGG^CCA
BcgI         1052  CGATTCTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         5257  CTTAAG               C^TTAAG
BsaBI        2765  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1230  GGCGTC               G[GA]^CG[CT]C
BsaWI        3918  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     2661  TGTACA               T^GTACA
BspDI         674  ATCGAT               AT^CGAT
BspHI        2247  TCATGA               T^CATGA
BsrBI        3217  CCGCTC               CCGCTC(-3/-3)
BsrGI        2661  TGTACA               T^GTACA
BstBI        3727  TTCGAA               TT^CGAA
BstEII       1528  GGTAACC              G^GT[ACGT]ACC
BstPI        1528  GGTAACC              G^GT[ACGT]ACC
ClaI          674  ATCGAT               AT^CGAT
Eam1105I      186  GACATTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1528  GGTAACC              G^GT[ACGT]ACC
EcoRI        4129  GAATTC               G^AATTC
Hin1I        1230  GGCGTC               G[GA]^CG[CT]C
HindIII      2785  AAGCTT               A^AGCTT
HpaI         1793  GTTAAC               GTT^AAC
KpnI          476  GGTACC               GGTAC^C
MamI         2765  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        5044  TGGCCA               TGG^CCA
MscI         5044  TGGCCA               TGG^CCA
NdeI         1915  CATATG               CA^TATG
NspV         3727  TTCGAA               TT^CGAA
PflFI         891  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2247  TCATGA               T^CATGA
SalI         4806  GTCGAC               G^TCGAC
SapI         5027  GAAGAGC              GCTCTTC(1/4)
ScaI         2419  AGTACT               AGT^ACT
SfuI         3727  TTCGAA               TT^CGAA
SmaI          930  CCCGGG               CCC^GGG
SpeI          269  ACTAGT               A^CTAGT
SspBI        2661  TGTACA               T^GTACA
StuI         4422  AGGCCT               AGG^CCT
SwaI          414  ATTTAAAT             ATTT^AAAT
Tth111I       891  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         930  CCCGGG               C^CCGGG
XmaI          930  CCCGGG               C^CCGGG
XmnI         5272  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AocI         2856  CCTTAGG              CC^T[ACGT]AGG
             4274  CCTGAGG             
Aor51HI      1701  AGCGCT               AGC^GCT
             2053  AGCGCT              
ApaI         1989  GGGCCC               GGGCC^C
             2076  GGGCCC              
AseI         2565  ATTAAT               AT^TAAT
             3766  ATTAAT              
AsnI         2565  ATTAAT               AT^TAAT
             3766  ATTAAT              
AvrII        2928  CCTAGG               C^CTAGG
             3641  CCTAGG              
BbrPI        1444  CACGTG               CAC^GTG
             3942  CACGTG              
BlnI         2928  CCTAGG               C^CTAGG
             3641  CCTAGG              
BlpI         1555  GCTGAGC              GC^T[ACGT]AGC
             3897  GCTCAGC             
Bpu1102I     1555  GCTGAGC              GC^T[ACGT]AGC
             3897  GCTCAGC             
BsmBI        1672  CGTCTC               CGTCTC(1/5)
             4779  GAGACG              
BsmI         4337  GAATGC               GAATGC(1/-1)
             4593  GAATGC              
BssSI         699  CACGAG               CACGAG(-5/-1)
             2963  CACGAG              
BstAPI       2206  GCACGGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3980  GCAGGGTGTGC         
BstXI        2437  CCAATGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4787  CCAGTCTTTTGG        
Bsu36I       2856  CCTTAGG              CC^T[ACGT]AGG
             4274  CCTGAGG             
CelII        1555  GCTGAGC              GC^T[ACGT]AGC
             3897  GCTCAGC             
DrdI         4781  GACGCTCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5229  GACTGGCGAGTC        
Eco47III     1701  AGCGCT               AGC^GCT
             2053  AGCGCT              
Eco81I       2856  CCTTAGG              CC^T[ACGT]AGG
             4274  CCTGAGG             
EcoNI        3204  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3666  CCTGGGGAAGG         
EcoRV        2406  GATATC               GAT^ATC
             4260  GATATC              
NcoI         1411  CCATGG               C^CATGG
             4490  CCATGG              
NspI         1754  GCATGT               [GA]CATG^[CT]
             3121  ACATGC              
PaeR7I       1768  CTCGAG               C^TCGAG
             3690  CTCGAG              
PflMI        2018  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2192  CCATTCCTTGG         
PmaCI        1444  CACGTG               CAC^GTG
             3942  CACGTG              
PmlI         1444  CACGTG               CAC^GTG
             3942  CACGTG              
PshBI        2565  ATTAAT               AT^TAAT
             3766  ATTAAT              
PvuII         481  CAGCTG               CAG^CTG
             3126  CAGCTG              
SacI          555  GAGCTC               GAGCT^C
             4116  GAGCTC              
SbfI          599  CCTGCAGG             CCTGCA^GG
             4196  CCTGCAGG            
SexAI        1339  ACCAGGT              A^CC[AT]GGT
             2097  ACCTGGT             
Sse8387I      599  CCTGCAGG             CCTGCA^GG
             4196  CCTGCAGG            
Van91I       2018  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2192  CCATTCCTTGG         
XhoI         1768  CTCGAG               C^TCGAG
             3690  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaAI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NsiI        PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XcmI        XhoII