##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Aor51HI AscI Asp718I AspEI AspI
AviII BalI BbrPI BglI BlpI
Bpu1102I BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII BssSI BstBI
CelII ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI HpaI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 82 CGCG CG^CG
AcyI 1179 GGCGCC G[GA]^CG[CT]C
AgeI 1496 ACCGGT A^CCGGT
Alw44I 1812 GTGCAC G^TGCAC
AlwNI 2015 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 4566 GGGCCC GGGCC^C
ApaLI 1812 GTGCAC G^TGCAC
AvaI 4360 CCCGAG C^[CT]CG[GA]G
BbeI 1179 GGCGCC GGCGC^C
BcgI 5869 CGAGGTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 410 GTATCC GTATCC(6/5)
BcnI 1238 CCGGG CC^[GC]GG
BsaBI 562 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1179 GGCGCC G[GA]^CG[CT]C
BsaI 4593 GAGACC GGTCTC(1/5)
BsoBI 4360 CCCGAG C^[CT]CG[GA]G
BstAPI 978 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3858 GGTTACC G^GT[ACGT]ACC
BstPI 3858 GGTTACC G^GT[ACGT]ACC
BstUI 82 CGCG CG^CG
BstXI 3445 CCATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 3443 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3858 GGTTACC G^GT[ACGT]ACC
HaeII 1179 GGCGCC [GA]GCGC^[CT]
Hin1I 1179 GGCGCC G[GA]^CG[CT]C
HindIII 4603 AAGCTT A^AGCTT
KasI 1179 GGCGCC G^GCGCC
MamI 562 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 5887 CCGCTG C[AC]G^C[GT]G
MvnI 82 CGCG CG^CG
NaeI 1036 GCCGGC GCC^GGC
NarI 1179 GGCGCC GG^CGCC
NciI 1238 CCGGG CC^[GC]GG
NdeI 2202 CATATG CA^TATG
NgoMIV 1036 GCCGGC G^CCGGC
PflMI 4638 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1496 ACCGGT A^CCGGT
SfoI 1179 GGCGCC GGC^GCC
StuI 5903 AGGCCT AGG^CCT
TaqI 2325 TCGA T^CGA
TthHB8I 2325 TCGA T^CGA
Van91I 4638 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AflII 3503 CTTAAG C^TTAAG
5065 CTTAAG
AseI 2548 ATTAAT AT^TAAT
5598 ATTAAT
AsnI 2548 ATTAAT AT^TAAT
5598 ATTAAT
AvrII 2749 CCTAGG C^CTAGG
5315 CCTAGG
BbsI 3875 GAAGAC GAAGAC(2/6)
5310 GTCTTC
BbvCI 4351 CCTCAGC CCTCAGC(-5/-2)
4709 CCTCAGC
BclI 744 TGATCA T^GATCA
2392 TGATCA
BfrI 3503 CTTAAG C^TTAAG
5065 CTTAAG
BglII 370 AGATCT A^GATCT
2134 AGATCT
BlnI 2749 CCTAGG C^CTAGG
5315 CCTAGG
BpuAI 3875 GAAGAC GAAGAC(2/6)
5310 GTCTTC
BsaAI 3268 TACGTG [CT]AC^GT[GA]
4559 CACGTA
BsaWI 1252 ACCGGA [AT]^CCGG[AT]
1496 ACCGGT
BseRI 542 CTCCTC GAGGAG(10/8)
4144 CTCCTC
BsmBI 1454 CGTCTC CGTCTC(1/5)
4256 GAGACG
BsmI 2967 GAATGC GAATGC(1/-1)
2242 GCATTC
Bsp1407I 2316 TGTACA T^GTACA
2443 TGTACA
BspHI 741 TCATGA T^CATGA
1922 TCATGA
BspMI 1222 GCAGGT ACCTGC(4/8)
3262 GCAGGT
BsrBI 31 GAGCGG CCGCTC(-3/-3)
37 GAGCGG
BsrFI 1036 GCCGGC [GA]^CCGG[CT]
1496 ACCGGT
BsrGI 2316 TGTACA T^GTACA
2443 TGTACA
Bst1107I 3142 GTATAC GTA^TAC
4058 GTATAC
BstZ17I 3142 GTATAC GTA^TAC
4058 GTATAC
CfoI 1180 GCGC GCG^C
1334 GCGC
Cfr10I 1036 GCCGGC [GA]^CCGG[CT]
1496 ACCGGT
EcoNI 1287 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2292 CCTTAAAAAGG
FauI 33 GCGGG CCCGC(4/6)
39 GCGGG
FbaI 744 TGATCA T^GATCA
2392 TGATCA
HgaI 80 GACGC GACGC(5/10)
1753 GCGTC
HhaI 1180 GCGC GCG^C
1334 GCGC
HinP1I 1180 GCGC G^CGC
1334 GCGC
HincII 678 GTTGAC GT[CT]^[GA]AC
5965 GTTGAC
HindII 678 GTTGAC GT[CT]^[GA]AC
5965 GTTGAC
NcoI 947 CCATGG C^CATGG
5912 CCATGG
PshBI 2548 ATTAAT AT^TAAT
5598 ATTAAT
PstI 782 CTGCAG CTGCA^G
985 CTGCAG
RcaI 741 TCATGA T^CATGA
1922 TCATGA
SapI 1664 GCTCTTC GCTCTTC(1/4)
4782 GCTCTTC
SexAI 1292 ACCAGGT A^CC[AT]GGT
3085 ACCTGGT
SspBI 2316 TGTACA T^GTACA
2443 TGTACA
XcmI 396 CCAGATTCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1428 CCAGAGCACACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaII BamHI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI