################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AvrII BciVI BclI BfrI BglI BglII BlnI BsaBI BsiWI BsmBI BspDI BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraIII Eco47III EcoNI EcoO65I EcoRI EcoRV FbaI FseI KspI MamI MluI NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SnaBI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AhdI 3119 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 497 CCTGAGG CC^T[ACGT]AGG AspEI 3119 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1742 GGATCC G^GATCC BbrPI 1804 CACGTG CAC^GTG BsmI 547 GAATGC GAATGC(1/-1) BsrFI 4471 GCCGGC [GA]^CCGG[CT] BssSI 1309 CACGAG CACGAG(-5/-1) BstBI 1119 TTCGAA TT^CGAA Bsu36I 497 CCTGAGG CC^T[ACGT]AGG Cfr10I 4471 GCCGGC [GA]^CCGG[CT] DrdI 1439 GACACGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3119 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 497 CCTGAGG CC^T[ACGT]AGG HpaI 4555 GTTAAC GTT^AAC NaeI 4471 GCCGGC GCC^GGC NdeI 5212 CATATG CA^TATG NgoMIV 4471 GCCGGC G^CCGGC NspV 1119 TTCGAA TT^CGAA PmaCI 1804 CACGTG CAC^GTG PmlI 1804 CACGTG CAC^GTG SapI 3970 GAAGAGC GCTCTTC(1/4) SfuI 1119 TTCGAA TT^CGAA SmaI 2479 CCCGGG CCC^GGG SpeI 4335 ACTAGT A^CTAGT StuI 352 AGGCCT AGG^CCT XmaCI 2479 CCCGGG C^CCGGG XmaI 2479 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccIII 2151 TCCGGA T^CCGGA 2430 TCCGGA Asp700I 1867 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2056 GAAGGTCTTC AspI 894 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4614 GACAGAGTC AvaI 1246 CTCGGG C^[CT]CG[GA]G 2479 CCCGGG AviII 579 TGCGCA TGC^GCA 723 TGCGCA BbvCI 2539 CCTCAGC CCTCAGC(-5/-2) 4707 CCTCAGC BsaI 976 GAGACC GGTCTC(1/5) 4809 GGTCTC BsaWI 2151 TCCGGA [AT]^CCGG[AT] 2430 TCCGGA BseAI 2151 TCCGGA T^CCGGA 2430 TCCGGA BsoBI 1246 CTCGGG C^[CT]CG[GA]G 2479 CCCGGG Bsp1407I 1149 TGTACA T^GTACA 4383 TGTACA BspEI 2151 TCCGGA T^CCGGA 2430 TCCGGA BspHI 816 TCATGA T^CATGA 2845 TCATGA BspLU11I 261 ACATGT A^CATGT 3877 ACATGT BsrBI 2410 CCGCTC CCGCTC(-3/-3) 1340 GAGCGG BsrGI 1149 TGTACA T^GTACA 4383 TGTACA BstYI 27 GGATCT [GA]^GATC[CT] 1742 GGATCC EagI 2176 CGGCCG C^GGCCG 5125 CGGCCG EclXI 2176 CGGCCG C^GGCCG 5125 CGGCCG Eco52I 2176 CGGCCG C^GGCCG 5125 CGGCCG EcoT22I 521 ATGCAT ATGCA^T 5156 ATGCAT FspI 579 TGCGCA TGC^GCA 723 TGCGCA HindIII 304 AAGCTT A^AGCTT 3452 AAGCTT MfeI 1777 CAATTG C^AATTG 5286 CAATTG MflI 27 GGATCT [GA]^GATC[CT] 1742 GGATCC MroI 2151 TCCGGA T^CCGGA 2430 TCCGGA MunI 1777 CAATTG C^AATTG 5286 CAATTG NsiI 521 ATGCAT ATGCA^T 5156 ATGCAT PflFI 894 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4614 GACAGAGTC PpuMI 2103 AGGACCC [GA]G^G[AT]CC[CT] 2211 AGGTCCC RcaI 816 TCATGA T^CATGA 2845 TCATGA ScaI 1917 AGTACT AGT^ACT 3068 AGTACT SspBI 1149 TGTACA T^GTACA 4383 TGTACA SspI 3776 AATATT AAT^ATT 4082 AATATT TaqI 801 TCGA T^CGA 1120 TCGA Tth111I 894 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4614 GACAGAGTC TthHB8I 801 TCGA T^CGA 1120 TCGA XhoII 27 GGATCT [GA]^GATC[CT] 1742 GGATCC XmnI 1867 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2056 GAAGGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI Asp718I AspHI AvaII BalI BanI BanII BbeI BbsI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI