##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
AvrII       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaBI       BsiWI       BsmBI       
BspDI       BssHII      Bst1107I    BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraIII      Eco47III    
EcoNI       EcoO65I     EcoRI       EcoRV       FbaI        
FseI        KspI        MamI        MluI        NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SgrAI       SnaBI       SphI        Sse8387I    
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AhdI         3119  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          497  CCTGAGG              CC^T[ACGT]AGG
AspEI        3119  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1742  GGATCC               G^GATCC
BbrPI        1804  CACGTG               CAC^GTG
BsmI          547  GAATGC               GAATGC(1/-1)
BsrFI        4471  GCCGGC               [GA]^CCGG[CT]
BssSI        1309  CACGAG               CACGAG(-5/-1)
BstBI        1119  TTCGAA               TT^CGAA
Bsu36I        497  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       4471  GCCGGC               [GA]^CCGG[CT]
DrdI         1439  GACACGCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3119  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        497  CCTGAGG              CC^T[ACGT]AGG
HpaI         4555  GTTAAC               GTT^AAC
NaeI         4471  GCCGGC               GCC^GGC
NdeI         5212  CATATG               CA^TATG
NgoMIV       4471  GCCGGC               G^CCGGC
NspV         1119  TTCGAA               TT^CGAA
PmaCI        1804  CACGTG               CAC^GTG
PmlI         1804  CACGTG               CAC^GTG
SapI         3970  GAAGAGC              GCTCTTC(1/4)
SfuI         1119  TTCGAA               TT^CGAA
SmaI         2479  CCCGGG               CCC^GGG
SpeI         4335  ACTAGT               A^CTAGT
StuI          352  AGGCCT               AGG^CCT
XmaCI        2479  CCCGGG               C^CCGGG
XmaI         2479  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccIII       2151  TCCGGA               T^CCGGA
             2430  TCCGGA              
Asp700I      1867  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2056  GAAGGTCTTC          
AspI          894  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4614  GACAGAGTC           
AvaI         1246  CTCGGG               C^[CT]CG[GA]G
             2479  CCCGGG              
AviII         579  TGCGCA               TGC^GCA
              723  TGCGCA              
BbvCI        2539  CCTCAGC              CCTCAGC(-5/-2)
             4707  CCTCAGC             
BsaI          976  GAGACC               GGTCTC(1/5)
             4809  GGTCTC              
BsaWI        2151  TCCGGA               [AT]^CCGG[AT]
             2430  TCCGGA              
BseAI        2151  TCCGGA               T^CCGGA
             2430  TCCGGA              
BsoBI        1246  CTCGGG               C^[CT]CG[GA]G
             2479  CCCGGG              
Bsp1407I     1149  TGTACA               T^GTACA
             4383  TGTACA              
BspEI        2151  TCCGGA               T^CCGGA
             2430  TCCGGA              
BspHI         816  TCATGA               T^CATGA
             2845  TCATGA              
BspLU11I      261  ACATGT               A^CATGT
             3877  ACATGT              
BsrBI        2410  CCGCTC               CCGCTC(-3/-3)
             1340  GAGCGG              
BsrGI        1149  TGTACA               T^GTACA
             4383  TGTACA              
BstYI          27  GGATCT               [GA]^GATC[CT]
             1742  GGATCC              
EagI         2176  CGGCCG               C^GGCCG
             5125  CGGCCG              
EclXI        2176  CGGCCG               C^GGCCG
             5125  CGGCCG              
Eco52I       2176  CGGCCG               C^GGCCG
             5125  CGGCCG              
EcoT22I       521  ATGCAT               ATGCA^T
             5156  ATGCAT              
FspI          579  TGCGCA               TGC^GCA
              723  TGCGCA              
HindIII       304  AAGCTT               A^AGCTT
             3452  AAGCTT              
MfeI         1777  CAATTG               C^AATTG
             5286  CAATTG              
MflI           27  GGATCT               [GA]^GATC[CT]
             1742  GGATCC              
MroI         2151  TCCGGA               T^CCGGA
             2430  TCCGGA              
MunI         1777  CAATTG               C^AATTG
             5286  CAATTG              
NsiI          521  ATGCAT               ATGCA^T
             5156  ATGCAT              
PflFI         894  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4614  GACAGAGTC           
PpuMI        2103  AGGACCC              [GA]G^G[AT]CC[CT]
             2211  AGGTCCC             
RcaI          816  TCATGA               T^CATGA
             2845  TCATGA              
ScaI         1917  AGTACT               AGT^ACT
             3068  AGTACT              
SspBI        1149  TGTACA               T^GTACA
             4383  TGTACA              
SspI         3776  AATATT               AAT^ATT
             4082  AATATT              
TaqI          801  TCGA                 T^CGA
             1120  TCGA                
Tth111I       894  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4614  GACAGAGTC           
TthHB8I       801  TCGA                 T^CGA
             1120  TCGA                
XhoII          27  GGATCT               [GA]^GATC[CT]
             1742  GGATCC              
XmnI         1867  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2056  GAAGGTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcsI        
AcyI        AfaI        AflIII      AluI        AlwI        
AlwNI       ApaI        ApoI        Asp718I     AspHI       
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvI        BcgI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        KpnI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmlI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI