################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccIII AclI AgeI Alw44I ApaI ApaLI AscI AvaI AviII AvrII BamHI BbrPI BcgI BlnI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstPI Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I EcoNI EcoO65I EcoRV FauI FseI FspI HpaI KspI MluI MroI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1107 GGTACC G^GTACC AcyI 58 GGCGCC G[GA]^CG[CT]C AflII 151 CTTAAG C^TTAAG AhdI 3404 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2776 CCTGAGG CC^T[ACGT]AGG Aor51HI 4538 AGCGCT AGC^GCT Asp718I 1107 GGTACC G^GTACC AspEI 3404 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 81 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1622 TGGCCA TGG^CCA BanII 1296 GAGCTC G[GA]GC[CT]^C BbeI 58 GGCGCC GGCGC^C BbvCI 922 GCTGAGG CCTCAGC(-5/-2) BfrI 151 CTTAAG C^TTAAG BglI 24 GCCGCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 3331 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3331 GCTAAGC GC^T[ACGT]AGC BsaAI 2251 TACGTA [CT]AC^GT[GA] BsaHI 58 GGCGCC G[GA]^CG[CT]C BsmI 3147 GCATTC GAATGC(1/-1) BspMI 1992 ACCTGC ACCTGC(4/8) Bst1107I 180 GTATAC GTA^TAC BstZ17I 180 GTATAC GTA^TAC Bsu36I 2776 CCTGAGG CC^T[ACGT]AGG CelII 3331 GCTAAGC GC^T[ACGT]AGC DraII 2402 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 3221 CCATGG C^C[GA][CT]GG EaeI 1622 TGGCCA [CT]^GGCC[GA] Eam1105I 3404 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4538 AGCGCT AGC^GCT Eco81I 2776 CCTGAGG CC^T[ACGT]AGG EcoO109I 2402 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 773 GAATTC G^AATTC EcoT14I 3221 CCATGG C^C[AT][AT]GG Hin1I 58 GGCGCC G[GA]^CG[CT]C HincII 112 GTTGAC GT[CT]^[GA]AC HindII 112 GTTGAC GT[CT]^[GA]AC KasI 58 GGCGCC G^GCGCC KpnI 1107 GGTACC GGTAC^C MfeI 224 CAATTG C^AATTG MluNI 1622 TGGCCA TGG^CCA MscI 1622 TGGCCA TGG^CCA MunI 224 CAATTG C^AATTG NarI 58 GGCGCC GG^CGCC NcoI 3221 CCATGG C^CATGG PflFI 81 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 555 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1296 GAGCTC GAGCT^C SapI 1603 GCTCTTC GCTCTTC(1/4) ScaI 913 AGTACT AGT^ACT SfoI 58 GGCGCC GGC^GCC SnaBI 2251 TACGTA TAC^GTA StuI 854 AGGCCT AGG^CCT StyI 3221 CCATGG C^C[AT][AT]GG Tth111I 81 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 555 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2384 CCAGTCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 109 CGCG CG^CG 1981 CGCG AlwNI 502 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 1175 CAGCAGCTG AseI 2521 ATTAAT AT^TAAT 5073 ATTAAT AsnI 2521 ATTAAT AT^TAAT 5073 ATTAAT AvaII 1714 GGACC G^G[AT]CC 2700 GGACC BanI 58 GGCGCC G^G[CT][GA]CC 1107 GGTACC BciVI 1230 GGATAC GTATCC(6/5) 2718 GTATCC BcnI 62 CCGGG CC^[GC]GG 90 CCCGG BpmI 3065 CTCCAG CTGGAG(16/14) 614 CTGGAG BsaI 3545 GAGACC GGTCTC(1/5) 412 GGTCTC BseRI 175 GAGGAG GAGGAG(10/8) 890 GAGGAG BspHI 1817 TCATGA T^CATGA 2304 TCATGA BstUI 109 CGCG CG^CG 1981 CGCG DraIII 1754 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1865 CACAATGTG EarI 1604 CTCTTC CTCTTC(1/4) 4932 GAAGAG HaeII 58 GGCGCC [GA]GCGC^[CT] 4538 AGCGCT HapII 62 CCGG C^CGG 91 CCGG HgaI 208 GACGC GACGC(5/10) 1665 GCGTC HpaII 62 CCGG C^CGG 91 CCGG Ksp632I 1604 CTCTTC CTCTTC(1/4) 4932 GAAGAG MspA1I 1178 CAGCTG C[AC]G^C[GT]G 4248 CAGCTG MspI 62 CCGG C^CGG 91 CCGG MvnI 109 CGCG CG^CG 1981 CGCG NciI 62 CCGGG CC^[GC]GG 90 CCCGG NdeI 1531 CATATG CA^TATG 3612 CATATG PshBI 2521 ATTAAT AT^TAAT 5073 ATTAAT PvuII 1178 CAGCTG CAG^CTG 4248 CAGCTG RcaI 1817 TCATGA T^CATGA 2304 TCATGA SpeI 2934 ACTAGT A^CTAGT 4211 ACTAGT SphI 2004 GCATGC GCATG^C 5061 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI BbsI BbvI BclI BfaI BglII BmyI BpuAI BsaBI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstBI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EcoRII EcoT22I FbaI Fnu4HI FokI HaeIII HhaI HinP1I HindIII HinfI HphI ItaI MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI NspV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI