##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        Alw44I      
ApaI        ApaLI       AscI        AvaI        AviII       
AvrII       BamHI       BbrPI       BcgI        BlnI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      BssSI       BstAPI      BstEII      BstPI       
Cfr10I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco52I      EcoNI       EcoO65I     EcoRV       
FauI        FseI        FspI        HpaI        KspI        
MluI        MroI        NaeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SgrAI       
SmaI        Sse8387I    SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I       1107  GGTACC               G^GTACC
AcyI           58  GGCGCC               G[GA]^CG[CT]C
AflII         151  CTTAAG               C^TTAAG
AhdI         3404  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2776  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      4538  AGCGCT               AGC^GCT
Asp718I      1107  GGTACC               G^GTACC
AspEI        3404  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI           81  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1622  TGGCCA               TGG^CCA
BanII        1296  GAGCTC               G[GA]GC[CT]^C
BbeI           58  GGCGCC               GGCGC^C
BbvCI         922  GCTGAGG              CCTCAGC(-5/-2)
BfrI          151  CTTAAG               C^TTAAG
BglI           24  GCCGCACGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3331  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     3331  GCTAAGC              GC^T[ACGT]AGC
BsaAI        2251  TACGTA               [CT]AC^GT[GA]
BsaHI          58  GGCGCC               G[GA]^CG[CT]C
BsmI         3147  GCATTC               GAATGC(1/-1)
BspMI        1992  ACCTGC               ACCTGC(4/8)
Bst1107I      180  GTATAC               GTA^TAC
BstZ17I       180  GTATAC               GTA^TAC
Bsu36I       2776  CCTGAGG              CC^T[ACGT]AGG
CelII        3331  GCTAAGC              GC^T[ACGT]AGC
DraII        2402  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI         3221  CCATGG               C^C[GA][CT]GG
EaeI         1622  TGGCCA               [CT]^GGCC[GA]
Eam1105I     3404  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4538  AGCGCT               AGC^GCT
Eco81I       2776  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     2402  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         773  GAATTC               G^AATTC
EcoT14I      3221  CCATGG               C^C[AT][AT]GG
Hin1I          58  GGCGCC               G[GA]^CG[CT]C
HincII        112  GTTGAC               GT[CT]^[GA]AC
HindII        112  GTTGAC               GT[CT]^[GA]AC
KasI           58  GGCGCC               G^GCGCC
KpnI         1107  GGTACC               GGTAC^C
MfeI          224  CAATTG               C^AATTG
MluNI        1622  TGGCCA               TGG^CCA
MscI         1622  TGGCCA               TGG^CCA
MunI          224  CAATTG               C^AATTG
NarI           58  GGCGCC               GG^CGCC
NcoI         3221  CCATGG               C^CATGG
PflFI          81  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         555  CCACCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         1296  GAGCTC               GAGCT^C
SapI         1603  GCTCTTC              GCTCTTC(1/4)
ScaI          913  AGTACT               AGT^ACT
SfoI           58  GGCGCC               GGC^GCC
SnaBI        2251  TACGTA               TAC^GTA
StuI          854  AGGCCT               AGG^CCT
StyI         3221  CCATGG               C^C[AT][AT]GG
Tth111I        81  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        555  CCACCAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2384  CCAGTCAGACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         109  CGCG                 CG^CG
             1981  CGCG                
AlwNI         502  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1175  CAGCAGCTG           
AseI         2521  ATTAAT               AT^TAAT
             5073  ATTAAT              
AsnI         2521  ATTAAT               AT^TAAT
             5073  ATTAAT              
AvaII        1714  GGACC                G^G[AT]CC
             2700  GGACC               
BanI           58  GGCGCC               G^G[CT][GA]CC
             1107  GGTACC              
BciVI        1230  GGATAC               GTATCC(6/5)
             2718  GTATCC              
BcnI           62  CCGGG                CC^[GC]GG
               90  CCCGG               
BpmI         3065  CTCCAG               CTGGAG(16/14)
              614  CTGGAG              
BsaI         3545  GAGACC               GGTCTC(1/5)
              412  GGTCTC              
BseRI         175  GAGGAG               GAGGAG(10/8)
              890  GAGGAG              
BspHI        1817  TCATGA               T^CATGA
             2304  TCATGA              
BstUI         109  CGCG                 CG^CG
             1981  CGCG                
DraIII       1754  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1865  CACAATGTG           
EarI         1604  CTCTTC               CTCTTC(1/4)
             4932  GAAGAG              
HaeII          58  GGCGCC               [GA]GCGC^[CT]
             4538  AGCGCT              
HapII          62  CCGG                 C^CGG
               91  CCGG                
HgaI          208  GACGC                GACGC(5/10)
             1665  GCGTC               
HpaII          62  CCGG                 C^CGG
               91  CCGG                
Ksp632I      1604  CTCTTC               CTCTTC(1/4)
             4932  GAAGAG              
MspA1I       1178  CAGCTG               C[AC]G^C[GT]G
             4248  CAGCTG              
MspI           62  CCGG                 C^CGG
               91  CCGG                
MvnI          109  CGCG                 CG^CG
             1981  CGCG                
NciI           62  CCGGG                CC^[GC]GG
               90  CCCGG               
NdeI         1531  CATATG               CA^TATG
             3612  CATATG              
PshBI        2521  ATTAAT               AT^TAAT
             5073  ATTAAT              
PvuII        1178  CAGCTG               CAG^CTG
             4248  CAGCTG              
RcaI         1817  TCATGA               T^CATGA
             2304  TCATGA              
SpeI         2934  ACTAGT               A^CTAGT
             4211  ACTAGT              
SphI         2004  GCATGC               GCATG^C
             5061  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        Asp700I     AspHI       
BbsI        BbvI        BclI        BfaI        BglII       
BmyI        BpuAI       BsaBI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    Bsp1407I    BspLU11I    BsrDI       BsrGI       
BsrI        BssKI       BstBI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EcoRII      EcoT22I     
FbaI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HindIII     HinfI       HphI        ItaI        
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NsiI        NspI        NspV        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfuI        SmlI        SspBI       SspI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI