##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaI ApaLI AscI AvaI AviII
AvrII BamHI BbrPI BcgI BlnI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstPI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoNI EcoO65I EcoRV
FauI FseI FspI HpaI KspI
MluI MroI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SgrAI
SmaI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1107 GGTACC G^GTACC
AcyI 58 GGCGCC G[GA]^CG[CT]C
AflII 151 CTTAAG C^TTAAG
AhdI 3404 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2776 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4538 AGCGCT AGC^GCT
Asp718I 1107 GGTACC G^GTACC
AspEI 3404 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 81 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1622 TGGCCA TGG^CCA
BanII 1296 GAGCTC G[GA]GC[CT]^C
BbeI 58 GGCGCC GGCGC^C
BbvCI 922 GCTGAGG CCTCAGC(-5/-2)
BfrI 151 CTTAAG C^TTAAG
BglI 24 GCCGCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3331 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3331 GCTAAGC GC^T[ACGT]AGC
BsaAI 2251 TACGTA [CT]AC^GT[GA]
BsaHI 58 GGCGCC G[GA]^CG[CT]C
BsmI 3147 GCATTC GAATGC(1/-1)
BspMI 1992 ACCTGC ACCTGC(4/8)
Bst1107I 180 GTATAC GTA^TAC
BstZ17I 180 GTATAC GTA^TAC
Bsu36I 2776 CCTGAGG CC^T[ACGT]AGG
CelII 3331 GCTAAGC GC^T[ACGT]AGC
DraII 2402 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 3221 CCATGG C^C[GA][CT]GG
EaeI 1622 TGGCCA [CT]^GGCC[GA]
Eam1105I 3404 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4538 AGCGCT AGC^GCT
Eco81I 2776 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2402 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 773 GAATTC G^AATTC
EcoT14I 3221 CCATGG C^C[AT][AT]GG
Hin1I 58 GGCGCC G[GA]^CG[CT]C
HincII 112 GTTGAC GT[CT]^[GA]AC
HindII 112 GTTGAC GT[CT]^[GA]AC
KasI 58 GGCGCC G^GCGCC
KpnI 1107 GGTACC GGTAC^C
MfeI 224 CAATTG C^AATTG
MluNI 1622 TGGCCA TGG^CCA
MscI 1622 TGGCCA TGG^CCA
MunI 224 CAATTG C^AATTG
NarI 58 GGCGCC GG^CGCC
NcoI 3221 CCATGG C^CATGG
PflFI 81 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 555 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1296 GAGCTC GAGCT^C
SapI 1603 GCTCTTC GCTCTTC(1/4)
ScaI 913 AGTACT AGT^ACT
SfoI 58 GGCGCC GGC^GCC
SnaBI 2251 TACGTA TAC^GTA
StuI 854 AGGCCT AGG^CCT
StyI 3221 CCATGG C^C[AT][AT]GG
Tth111I 81 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 555 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2384 CCAGTCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 109 CGCG CG^CG
1981 CGCG
AlwNI 502 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1175 CAGCAGCTG
AseI 2521 ATTAAT AT^TAAT
5073 ATTAAT
AsnI 2521 ATTAAT AT^TAAT
5073 ATTAAT
AvaII 1714 GGACC G^G[AT]CC
2700 GGACC
BanI 58 GGCGCC G^G[CT][GA]CC
1107 GGTACC
BciVI 1230 GGATAC GTATCC(6/5)
2718 GTATCC
BcnI 62 CCGGG CC^[GC]GG
90 CCCGG
BpmI 3065 CTCCAG CTGGAG(16/14)
614 CTGGAG
BsaI 3545 GAGACC GGTCTC(1/5)
412 GGTCTC
BseRI 175 GAGGAG GAGGAG(10/8)
890 GAGGAG
BspHI 1817 TCATGA T^CATGA
2304 TCATGA
BstUI 109 CGCG CG^CG
1981 CGCG
DraIII 1754 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1865 CACAATGTG
EarI 1604 CTCTTC CTCTTC(1/4)
4932 GAAGAG
HaeII 58 GGCGCC [GA]GCGC^[CT]
4538 AGCGCT
HapII 62 CCGG C^CGG
91 CCGG
HgaI 208 GACGC GACGC(5/10)
1665 GCGTC
HpaII 62 CCGG C^CGG
91 CCGG
Ksp632I 1604 CTCTTC CTCTTC(1/4)
4932 GAAGAG
MspA1I 1178 CAGCTG C[AC]G^C[GT]G
4248 CAGCTG
MspI 62 CCGG C^CGG
91 CCGG
MvnI 109 CGCG CG^CG
1981 CGCG
NciI 62 CCGGG CC^[GC]GG
90 CCCGG
NdeI 1531 CATATG CA^TATG
3612 CATATG
PshBI 2521 ATTAAT AT^TAAT
5073 ATTAAT
PvuII 1178 CAGCTG CAG^CTG
4248 CAGCTG
RcaI 1817 TCATGA T^CATGA
2304 TCATGA
SpeI 2934 ACTAGT A^CTAGT
4211 ACTAGT
SphI 2004 GCATGC GCATG^C
5061 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
BbsI BbvI BclI BfaI BglII
BmyI BpuAI BsaBI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI
BsrI BssKI BstBI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EcoRII EcoT22I
FbaI Fnu4HI FokI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI NspV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI SspBI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI