##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AvaI AviII AvrII
BcgI BfrI BlnI BsaWI BseAI
BsiWI BsoBI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I FseI FspI
HincII HindII HpaI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 150 GACGTC GACGT^C
Acc65I 2086 GGTACC G^GTACC
Asp700I 2483 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2086 GGTACC G^GTACC
AspI 2171 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1966 GGATCC G^GATCC
BbvCI 2229 GCTGAGG CCTCAGC(-5/-2)
BciVI 2466 GGATAC GTATCC(6/5)
BclI 3240 TGATCA T^GATCA
BglI 1602 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2034 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2034 GCTCAGC GC^T[ACGT]AGC
BsaBI 519 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 128 CGACCG CG[GA][CT]^CG
BsmBI 2550 CGTCTC CGTCTC(1/5)
BspDI 4515 ATCGAT AT^CGAT
BspLU11I 4183 ACATGT A^CATGT
BsrDI 613 GCAATG GCAATG(2/0)
BstAPI 2259 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2034 GCTCAGC GC^T[ACGT]AGC
ClaI 4515 ATCGAT AT^CGAT
DraIII 4911 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4287 GACATAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1173 GATATC GAT^ATC
FbaI 3240 TGATCA T^GATCA
HindIII 1976 AAGCTT A^AGCTT
KpnI 2086 GGTACC GGTAC^C
MamI 519 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1057 GCCGGC GCC^GGC
NdeI 3266 CATATG CA^TATG
NgoMIV 1057 GCCGGC G^CCGGC
PflFI 2171 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 368 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 26 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1837 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4368 CTGCAG CTGCA^G
SpeI 3226 ACTAGT A^CTAGT
SphI 1786 GCATGC GCATG^C
SspI 3422 AATATT AAT^ATT
StuI 1994 AGGCCT AGG^CCT
Tth111I 2171 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 368 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2483 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1328 CCTGAGG CC^T[ACGT]AGG
1467 CCTCAGG
AseI 3339 ATTAAT AT^TAAT
4923 ATTAAT
AsnI 3339 ATTAAT AT^TAAT
4923 ATTAAT
AspHI 650 GAGCAC G[AT]GC[AT]^C
2118 GAGCAC
BalI 238 TGGCCA TGG^CCA
3943 TGGCCA
BbeI 231 GGCGCC GGCGC^C
1518 GGCGCC
BbrPI 1794 CACGTG CAC^GTG
2091 CACGTG
BsiHKAI 650 GAGCAC G[AT]GC[AT]^C
2118 GAGCAC
Bsp1407I 2215 TGTACA T^GTACA
4463 TGTACA
BspHI 961 TCATGA T^CATGA
1985 TCATGA
BsrGI 2215 TGTACA T^GTACA
4463 TGTACA
Bsu36I 1328 CCTGAGG CC^T[ACGT]AGG
1467 CCTCAGG
DraII 26 AGGTCCC [GA]G^G[ACGT]CC[CT]
158 GGGGCCT
Eco81I 1328 CCTGAGG CC^T[ACGT]AGG
1467 CCTCAGG
EcoO109I 26 AGGTCCC [GA]G^G[ACGT]CC[CT]
158 GGGGCCT
KasI 231 GGCGCC G^GCGCC
1518 GGCGCC
KspI 215 CCGCGG CCGC^GG
2131 CCGCGG
MluNI 238 TGGCCA TGG^CCA
3943 TGGCCA
MscI 238 TGGCCA TGG^CCA
3943 TGGCCA
MslI 3050 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3167 CATTTCTGTG
NarI 231 GGCGCC GG^CGCC
1518 GGCGCC
PmaCI 1794 CACGTG CAC^GTG
2091 CACGTG
PmlI 1794 CACGTG CAC^GTG
2091 CACGTG
PshBI 3339 ATTAAT AT^TAAT
4923 ATTAAT
RcaI 961 TCATGA T^CATGA
1985 TCATGA
SacII 215 CCGCGG CCGC^GG
2131 CCGCGG
ScaI 921 AGTACT AGT^ACT
3812 AGTACT
SfoI 231 GGCGCC GGC^GCC
1518 GGCGCC
SmlI 1081 CTCAAG C^T[CT][GA]AG
1588 CTCAAG
SspBI 2215 TGTACA T^GTACA
4463 TGTACA
TaqI 1482 TCGA T^CGA
4516 TCGA
TthHB8I 1482 TCGA T^CGA
4516 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII