##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI AocI AscI AseI AsnI
AspEI AvrII BclI BfrI BglII
BlnI BsaBI BsiWI BsmI BspLU11I
BssHII Bst1107I BstBI BstZ17I Bsu36I
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI HindIII HpaI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 3886 TCCGGA T^CCGGA
AcsI 1193 GAATTC [GA]^AATT[CT]
Alw44I 1423 GTGCAC G^TGCAC
ApaLI 1423 GTGCAC G^TGCAC
ApoI 1193 GAATTC [GA]^AATT[CT]
BseAI 3886 TCCGGA T^CCGGA
BsiEI 1131 CGGTCG CG[GA][CT]^CG
BsmBI 2801 CGTCTC CGTCTC(1/5)
Bsp1407I 4078 TGTACA T^GTACA
BspDI 271 ATCGAT AT^CGAT
BspEI 3886 TCCGGA T^CCGGA
BspHI 1151 TCATGA T^CATGA
BsrGI 4078 TGTACA T^GTACA
BssSI 2722 CTCGTG CACGAG(-5/-1)
ClaI 271 ATCGAT AT^CGAT
EcoRI 1193 GAATTC G^AATTC
KspI 5545 CCGCGG CCGC^GG
MroI 3886 TCCGGA T^CCGGA
RcaI 1151 TCATGA T^CATGA
SacII 5545 CCGCGG CCGC^GG
ScaI 1628 AGTACT AGT^ACT
SexAI 52 ACCTGGT A^CC[AT]GGT
SspBI 4078 TGTACA T^GTACA
XbaI 2287 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1244 GGTACC G^GTACC
1811 GGTACC
Aor51HI 1345 AGCGCT AGC^GCT
4449 AGCGCT
Asp700I 1614 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3674 GAAGCCCTTC
Asp718I 1244 GGTACC G^GTACC
1811 GGTACC
AviII 1694 TGCGCA TGC^GCA
2345 TGCGCA
BamHI 3889 GGATCC G^GATCC
5541 GGATCC
BbeI 312 GGCGCC GGCGC^C
4176 GGCGCC
BbsI 4301 GAAGAC GAAGAC(2/6)
5220 GAAGAC
BbvCI 488 CCTCAGC CCTCAGC(-5/-2)
5102 GCTGAGG
BciVI 665 GTATCC GTATCC(6/5)
5532 GTATCC
BglI 3960 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5254 GCCATCATGGC
BlpI 2472 GCTGAGC GC^T[ACGT]AGC
3969 GCTGAGC
Bpu1102I 2472 GCTGAGC GC^T[ACGT]AGC
3969 GCTGAGC
BpuAI 4301 GAAGAC GAAGAC(2/6)
5220 GAAGAC
BsrDI 179 GCAATG GCAATG(2/0)
1418 GCAATG
BstAPI 1686 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5447 GCAGCCACTGC
CelII 2472 GCTGAGC GC^T[ACGT]AGC
3969 GCTGAGC
DrdI 2610 GACCCGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5354 GACAGATAAGTC
Eco47III 1345 AGCGCT AGC^GCT
4449 AGCGCT
EcoNI 1083 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4646 CCTGGCGGAGG
FspI 1694 TGCGCA TGC^GCA
2345 TGCGCA
HincII 90 GTTGAC GT[CT]^[GA]AC
1033 GTCAAC
HindII 90 GTTGAC GT[CT]^[GA]AC
1033 GTCAAC
KasI 312 GGCGCC G^GCGCC
4176 GGCGCC
KpnI 1244 GGTACC GGTAC^C
1811 GGTACC
NaeI 187 GCCGGC GCC^GGC
4006 GCCGGC
NarI 312 GGCGCC GG^CGCC
4176 GGCGCC
NgoMIV 187 GCCGGC G^CCGGC
4006 GCCGGC
NheI 291 GCTAGC G^CTAGC
5098 GCTAGC
SbfI 2304 CCTGCAGG CCTGCA^GG
3764 CCTGCAGG
SfoI 312 GGCGCC GGC^GCC
4176 GGCGCC
SphI 2240 GCATGC GCATG^C
2675 GCATGC
Sse8387I 2304 CCTGCAGG CCTGCA^GG
3764 CCTGCAGG
XmnI 1614 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3674 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbvI BcgI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI