##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Aor51HI ApaI AscI AspEI
AviII BbrPI BcgI BciVI BcnI
BglI BlpI Bpu1102I BseAI BsiEI
BsiWI BspEI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI FspI HpaI
KspI MluI MroI MvnI NaeI
NciI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI Sse8387I SwaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 665 GGCGCC G[GA]^CG[CT]C
AflIII 1543 ACATGT A^C[GA][CT]GT
AocI 791 CCTGAGG CC^T[ACGT]AGG
AvaI 1157 CTCGAG C^[CT]CG[GA]G
AvrII 2012 CCTAGG C^CTAGG
BamHI 4745 GGATCC G^GATCC
BbeI 665 GGCGCC GGCGC^C
BbvCI 3295 GCTGAGG CCTCAGC(-5/-2)
BglII 2484 AGATCT A^GATCT
BlnI 2012 CCTAGG C^CTAGG
BsaAI 4174 TACGTA [CT]AC^GT[GA]
BsaBI 5694 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 665 GGCGCC G[GA]^CG[CT]C
BsaWI 615 ACCGGA [AT]^CCGG[AT]
BsmBI 214 GAGACG CGTCTC(1/5)
BsoBI 1157 CTCGAG C^[CT]CG[GA]G
BspDI 2864 ATCGAT AT^CGAT
BspHI 5581 TCATGA T^CATGA
BspLU11I 1543 ACATGT A^CATGT
BsrBI 542 GAGCGG CCGCTC(-3/-3)
BsrDI 3291 CATTGC GCAATG(2/0)
BssSI 4885 CACGAG CACGAG(-5/-1)
Bsu36I 791 CCTGAGG CC^T[ACGT]AGG
CfoI 666 GCGC GCG^C
ClaI 2864 ATCGAT AT^CGAT
DraIII 2105 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 807 CCATGG C^C[GA][CT]GG
Eco81I 791 CCTGAGG CC^T[ACGT]AGG
HaeII 665 GGCGCC [GA]GCGC^[CT]
HapII 616 CCGG C^CGG
HgaI 216 GACGC GACGC(5/10)
HhaI 666 GCGC GCG^C
Hin1I 665 GGCGCC G[GA]^CG[CT]C
HinP1I 666 GCGC G^CGC
HincII 5930 GTTGAC GT[CT]^[GA]AC
HindII 5930 GTTGAC GT[CT]^[GA]AC
HindIII 2369 AAGCTT A^AGCTT
HpaII 616 CCGG C^CGG
KasI 665 GGCGCC G^GCGCC
MamI 5694 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 616 CCGG C^CGG
NarI 665 GGCGCC GG^CGCC
NcoI 807 CCATGG C^CATGG
PaeR7I 1157 CTCGAG C^TCGAG
PshAI 1759 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5581 TCATGA T^CATGA
SacI 671 GAGCTC GAGCT^C
SexAI 5034 ACCTGGT A^CC[AT]GGT
SfoI 665 GGCGCC GGC^GCC
SnaBI 4174 TACGTA TAC^GTA
SpeI 4983 ACTAGT A^CTAGT
SphI 1207 GCATGC GCATG^C
StuI 2973 AGGCCT AGG^CCT
XhoI 1157 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 4869 GGTACC G^GTACC
4880 GGTACC
Alw44I 963 GTGCAC G^TGCAC
5534 GTGCAC
ApaLI 963 GTGCAC G^TGCAC
5534 GTGCAC
Asp718I 4869 GGTACC G^GTACC
4880 GGTACC
AspI 469 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5406 GACCTCGTC
BalI 600 TGGCCA TGG^CCA
2049 TGGCCA
BanII 671 GAGCTC G[GA]GC[CT]^C
1569 GAGCCC
BsaI 1968 GGTCTC GGTCTC(1/5)
2537 GGTCTC
Bsp1407I 1462 TGTACA T^GTACA
3056 TGTACA
BsrGI 1462 TGTACA T^GTACA
3056 TGTACA
BstXI 2929 CCAAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4866 CCAGGTACCTGG
DraII 467 GGGACCC [GA]G^G[ACGT]CC[CT]
498 AGGTCCT
EaeI 600 TGGCCA [CT]^GGCC[GA]
2049 TGGCCA
EcoO109I 467 GGGACCC [GA]G^G[ACGT]CC[CT]
498 AGGTCCT
EcoT22I 1908 ATGCAT ATGCA^T
5856 ATGCAT
KpnI 4869 GGTACC GGTAC^C
4880 GGTACC
MfeI 1373 CAATTG C^AATTG
3981 CAATTG
MluNI 600 TGGCCA TGG^CCA
2049 TGGCCA
MscI 600 TGGCCA TGG^CCA
2049 TGGCCA
MunI 1373 CAATTG C^AATTG
3981 CAATTG
NdeI 3052 CATATG CA^TATG
5859 CATATG
NsiI 1908 ATGCAT ATGCA^T
5856 ATGCAT
PflFI 469 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5406 GACCTCGTC
PpuMI 467 GGGACCC [GA]G^G[AT]CC[CT]
498 AGGTCCT
SspBI 1462 TGTACA T^GTACA
3056 TGTACA
Tth111I 469 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5406 GACCTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BbsI BbvI BclI BfaI BfrI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmnI