##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AocI AscI AseI AsnI AspEI
AspI AviII BcgI BciVI BfrI
BglII BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI Eam1105I
Eco81I EcoRV FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MunI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 5211 TCCGGA T^CCGGA
Alw44I 4919 GTGCAC G^TGCAC
ApaLI 4919 GTGCAC G^TGCAC
BbsI 1426 GAAGAC GAAGAC(2/6)
BclI 3204 TGATCA T^GATCA
BpuAI 1426 GAAGAC GAAGAC(2/6)
BseAI 5211 TCCGGA T^CCGGA
BsiEI 1126 CGGCCG CG[GA][CT]^CG
BspEI 5211 TCCGGA T^CCGGA
BsrDI 2507 GCAATG GCAATG(2/0)
BstAPI 1213 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 548 GACGGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1126 CGGCCG C^GGCCG
EclXI 1126 CGGCCG C^GGCCG
Eco52I 1126 CGGCCG C^GGCCG
EcoRI 3162 GAATTC G^AATTC
EcoT22I 1552 ATGCAT ATGCA^T
FbaI 3204 TGATCA T^GATCA
MroI 5211 TCCGGA T^CCGGA
NaeI 3120 GCCGGC GCC^GGC
NdeI 4234 CATATG CA^TATG
NgoMIV 3120 GCCGGC G^CCGGC
NheI 5163 GCTAGC G^CTAGC
NsiI 1552 ATGCAT ATGCA^T
PshAI 2935 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 4524 ACCAGGT A^CC[AT]GGT
SmaI 3958 CCCGGG CCC^GGG
SphI 930 GCATGC GCATG^C
XmaCI 3958 CCCGGG C^CCGGG
XmaI 3958 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1464 GGTACC G^GTACC
5112 GGTACC
AccI 727 GTCTAC GT^[AC][GT]AC
2206 GTCTAC
AflIII 622 ACACGT A^C[GA][CT]GT
4820 ACGTGT
Asp718I 1464 GGTACC G^GTACC
5112 GGTACC
AvaI 1414 CTCGGG C^[CT]CG[GA]G
3958 CCCGGG
AvrII 2565 CCTAGG C^CTAGG
4775 CCTAGG
BbeI 280 GGCGCC GGCGC^C
3588 GGCGCC
BlnI 2565 CCTAGG C^CTAGG
4775 CCTAGG
BsaWI 1335 ACCGGA [AT]^CCGG[AT]
5211 TCCGGA
BsoBI 1414 CTCGGG C^[CT]CG[GA]G
3958 CCCGGG
Bsp1407I 250 TGTACA T^GTACA
1065 TGTACA
BsrFI 315 GCCGGT [GA]^CCGG[CT]
3120 GCCGGC
BsrGI 250 TGTACA T^GTACA
1065 TGTACA
BstXI 637 CCACCCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2035 CCAGGAGCTTGG
Cfr10I 315 GCCGGT [GA]^CCGG[CT]
3120 GCCGGC
DraI 3192 TTTAAA TTT^AAA
4209 TTTAAA
HgaI 555 GCGTC GACGC(5/10)
2204 GCGTC
HincII 569 GTCAAC GT[CT]^[GA]AC
4813 GTTGAC
HindII 569 GTCAAC GT[CT]^[GA]AC
4813 GTTGAC
KasI 280 GGCGCC G^GCGCC
3588 GGCGCC
KpnI 1464 GGTACC GGTAC^C
5112 GGTACC
NarI 280 GGCGCC GG^CGCC
3588 GGCGCC
SfiI 2100 GGCCCTTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5273 GGCCCACAGGGCC
SfoI 280 GGCGCC GGC^GCC
3588 GGCGCC
SspBI 250 TGTACA T^GTACA
1065 TGTACA
TfiI 1967 GAATC G^A[AT]TC
2857 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI Asp700I AspHI AvaII BalI
BamHI BanI BanII BbrPI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI