##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BamHI
BbrPI BciVI BfrI BglII BsaBI
BsiWI BsmBI Bsp1407I BspHI BsrGI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I CpoI DraIII DrdI Eam1105I
FseI FspI HpaI KpnI KspI
MamI MflI MluI NcoI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 1103 CGCG CG^CG
AcyI 1166 GGCGCC G[GA]^CG[CT]C
Aor51HI 1082 AGCGCT AGC^GCT
AspI 2105 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1166 GGCGCC GGCGC^C
BcgI 1901 GCAGGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2066 TGATCA T^GATCA
BlpI 3797 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3797 GCTGAGC GC^T[ACGT]AGC
BsaAI 422 TACGTA [CT]AC^GT[GA]
BsaHI 1166 GGCGCC G[GA]^CG[CT]C
BsiEI 578 CGGCCG CG[GA][CT]^CG
BspDI 2677 ATCGAT AT^CGAT
BssSI 1189 CACGAG CACGAG(-5/-1)
BstUI 1103 CGCG CG^CG
CelII 3797 GCTGAGC GC^T[ACGT]AGC
ClaI 2677 ATCGAT AT^CGAT
DraI 1975 TTTAAA TTT^AAA
DsaI 1284 CCACGG C^C[GA][CT]GG
EagI 578 CGGCCG C^GGCCG
EclXI 578 CGGCCG C^GGCCG
Eco47III 1082 AGCGCT AGC^GCT
Eco52I 578 CGGCCG C^GGCCG
EcoRI 2374 GAATTC G^AATTC
EcoRV 1289 GATATC GAT^ATC
EcoT22I 1092 ATGCAT ATGCA^T
FbaI 2066 TGATCA T^GATCA
HgaI 4742 GACGC GACGC(5/10)
Hin1I 1166 GGCGCC G[GA]^CG[CT]C
KasI 1166 GGCGCC G^GCGCC
MvnI 1103 CGCG CG^CG
NaeI 2900 GCCGGC GCC^GGC
NarI 1166 GGCGCC GG^CGCC
NdeI 3155 CATATG CA^TATG
NgoMIV 2900 GCCGGC G^CCGGC
NheI 3361 GCTAGC G^CTAGC
NsiI 1092 ATGCAT ATGCA^T
PflFI 2105 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 210 AGTACT AGT^ACT
SfoI 1166 GGCGCC GGC^GCC
SnaBI 422 TACGTA TAC^GTA
SpeI 523 ACTAGT A^CTAGT
Tth111I 2105 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 400 TCCGGA T^CCGGA
1063 TCCGGA
AcsI 1058 AAATTT [GA]^AATT[CT]
2374 GAATTC
AflIII 57 ACATGT A^C[GA][CT]GT
724 ACATGT
AocI 928 CCTGAGG CC^T[ACGT]AGG
3223 CCTCAGG
ApoI 1058 AAATTT [GA]^AATT[CT]
2374 GAATTC
AvaI 616 CTCGGG C^[CT]CG[GA]G
2241 CCCGAG
AvrII 2332 CCTAGG C^CTAGG
2446 CCTAGG
BlnI 2332 CCTAGG C^CTAGG
2446 CCTAGG
BseAI 400 TCCGGA T^CCGGA
1063 TCCGGA
BsmI 2784 GAATGC GAATGC(1/-1)
1638 GCATTC
BsoBI 616 CTCGGG C^[CT]CG[GA]G
2241 CCCGAG
BspEI 400 TCCGGA T^CCGGA
1063 TCCGGA
BspLU11I 57 ACATGT A^CATGT
724 ACATGT
BsrBI 4720 CCGCTC CCGCTC(-3/-3)
3438 GAGCGG
BsrDI 4298 CATTGC GCAATG(2/0)
693 GCAATG
BsrFI 384 ACCGGC [GA]^CCGG[CT]
2900 GCCGGC
Bsu36I 928 CCTGAGG CC^T[ACGT]AGG
3223 CCTCAGG
Cfr10I 384 ACCGGC [GA]^CCGG[CT]
2900 GCCGGC
Eco81I 928 CCTGAGG CC^T[ACGT]AGG
3223 CCTCAGG
EcoNI 294 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3597 CCTGGCAGAGG
MaeII 423 ACGT A^CGT
1997 ACGT
MfeI 3306 CAATTG C^AATTG
4746 CAATTG
MroI 400 TCCGGA T^CCGGA
1063 TCCGGA
MunI 3306 CAATTG C^AATTG
4746 CAATTG
SacI 1007 GAGCTC GAGCT^C
3709 GAGCTC
TaqI 166 TCGA T^CGA
2678 TCGA
TthHB8I 166 TCGA T^CGA
2678 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI ApaI ApaLI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI