################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AscI AseI AsnI Asp718I AspI AvrII BbrPI BciVI BclI BfaI BfrI BglII BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoRI EcoRV EcoT22I FbaI HindIII HpaI KpnI MaeI MamI MluI MroI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SalI SapI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 271 GAATTT [GA]^AATT[CT] AhdI 3061 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 847 GTGCAC G^TGCAC Aor51HI 2976 AGCGCT AGC^GCT ApaLI 847 GTGCAC G^TGCAC ApoI 271 GAATTT [GA]^AATT[CT] Asp700I 4437 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3061 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2387 TGGCCA TGG^CCA BamHI 971 GGATCC G^GATCC BcgI 4457 CGACCCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 844 TACGTG [CT]AC^GT[GA] BsaWI 3519 ACCGGA [AT]^CCGG[AT] BsmI 831 GCATTC GAATGC(1/-1) BssSI 3480 CACGAG CACGAG(-5/-1) BstAPI 1372 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 971 GGATCC [GA]^GATC[CT] DraI 4913 TTTAAA TTT^AAA DraIII 1373 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3061 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2976 AGCGCT AGC^GCT FseI 1521 GGCCGGCC GGCCGG^CC MfeI 1273 CAATTG C^AATTG MflI 971 GGATCC [GA]^GATC[CT] MluNI 2387 TGGCCA TGG^CCA MscI 2387 TGGCCA TGG^CCA MseI 4914 TTAA T^TAA MunI 1273 CAATTG C^AATTG NcoI 2222 CCATGG C^CATGG NspI 1161 GCATGT [GA]CATG^[CT] PleI 2179 GACTC GAGTC(4/5) PshAI 2572 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 3185 CGATCG CGAT^CG SbfI 465 CCTGCAGG CCTGCA^GG ScaI 1670 AGTACT AGT^ACT Sse8387I 465 CCTGCAGG CCTGCA^GG StuI 2403 AGGCCT AGG^CCT Tru9I 4914 TTAA T^TAA XhoII 971 GGATCC [GA]^GATC[CT] XmnI 4437 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AocI 1759 CCTCAGG CC^T[ACGT]AGG 3709 CCTCAGG AviII 866 TGCGCA TGC^GCA 899 TGCGCA BlpI 1878 GCTGAGC GC^T[ACGT]AGC 4170 GCTGAGC Bpu1102I 1878 GCTGAGC GC^T[ACGT]AGC 4170 GCTGAGC BstEII 1071 GGTGACC G^GT[ACGT]ACC 1461 GGTCACC BstPI 1071 GGTGACC G^GT[ACGT]ACC 1461 GGTCACC Bsu36I 1759 CCTCAGG CC^T[ACGT]AGG 3709 CCTCAGG CelII 1878 GCTGAGC GC^T[ACGT]AGC 4170 GCTGAGC Eco81I 1759 CCTCAGG CC^T[ACGT]AGG 3709 CCTCAGG EcoNI 2655 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4933 CCTTTTGGAGG EcoO65I 1071 GGTGACC G^GT[ACGT]ACC 1461 GGTCACC FspI 866 TGCGCA TGC^GCA 899 TGCGCA HincII 698 GTCAAC GT[CT]^[GA]AC 4209 GTCAAC HindII 698 GTCAAC GT[CT]^[GA]AC 4209 GTCAAC MaeII 845 ACGT A^CGT 4127 ACGT PaeR7I 770 CTCGAG C^TCGAG 1489 CTCGAG PvuII 1792 CAGCTG CAG^CTG 2641 CAGCTG XhoI 770 CTCGAG C^TCGAG 1489 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PpuMI PstI RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmaCI XmaI