##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AscI        AseI        AsnI        
Asp718I     AspI        AvrII       BbrPI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BsaBI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        EcoRI       EcoRV       EcoT22I     FbaI        
HindIII     HpaI        KpnI        MaeI        MamI        
MluI        MroI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    RcaI        RsrII       SalI        SapI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          271  GAATTT               [GA]^AATT[CT]
AhdI         3061  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        847  GTGCAC               G^TGCAC
Aor51HI      2976  AGCGCT               AGC^GCT
ApaLI         847  GTGCAC               G^TGCAC
ApoI          271  GAATTT               [GA]^AATT[CT]
Asp700I      4437  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3061  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         2387  TGGCCA               TGG^CCA
BamHI         971  GGATCC               G^GATCC
BcgI         4457  CGACCCCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI         844  TACGTG               [CT]AC^GT[GA]
BsaWI        3519  ACCGGA               [AT]^CCGG[AT]
BsmI          831  GCATTC               GAATGC(1/-1)
BssSI        3480  CACGAG               CACGAG(-5/-1)
BstAPI       1372  GCACACAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         971  GGATCC               [GA]^GATC[CT]
DraI         4913  TTTAAA               TTT^AAA
DraIII       1373  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3061  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2976  AGCGCT               AGC^GCT
FseI         1521  GGCCGGCC             GGCCGG^CC
MfeI         1273  CAATTG               C^AATTG
MflI          971  GGATCC               [GA]^GATC[CT]
MluNI        2387  TGGCCA               TGG^CCA
MscI         2387  TGGCCA               TGG^CCA
MseI         4914  TTAA                 T^TAA
MunI         1273  CAATTG               C^AATTG
NcoI         2222  CCATGG               C^CATGG
NspI         1161  GCATGT               [GA]CATG^[CT]
PleI         2179  GACTC                GAGTC(4/5)
PshAI        2572  GACCCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         3185  CGATCG               CGAT^CG
SbfI          465  CCTGCAGG             CCTGCA^GG
ScaI         1670  AGTACT               AGT^ACT
Sse8387I      465  CCTGCAGG             CCTGCA^GG
StuI         2403  AGGCCT               AGG^CCT
Tru9I        4914  TTAA                 T^TAA
XhoII         971  GGATCC               [GA]^GATC[CT]
XmnI         4437  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AocI         1759  CCTCAGG              CC^T[ACGT]AGG
             3709  CCTCAGG             
AviII         866  TGCGCA               TGC^GCA
              899  TGCGCA              
BlpI         1878  GCTGAGC              GC^T[ACGT]AGC
             4170  GCTGAGC             
Bpu1102I     1878  GCTGAGC              GC^T[ACGT]AGC
             4170  GCTGAGC             
BstEII       1071  GGTGACC              G^GT[ACGT]ACC
             1461  GGTCACC             
BstPI        1071  GGTGACC              G^GT[ACGT]ACC
             1461  GGTCACC             
Bsu36I       1759  CCTCAGG              CC^T[ACGT]AGG
             3709  CCTCAGG             
CelII        1878  GCTGAGC              GC^T[ACGT]AGC
             4170  GCTGAGC             
Eco81I       1759  CCTCAGG              CC^T[ACGT]AGG
             3709  CCTCAGG             
EcoNI        2655  CCTGCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4933  CCTTTTGGAGG         
EcoO65I      1071  GGTGACC              G^GT[ACGT]ACC
             1461  GGTCACC             
FspI          866  TGCGCA               TGC^GCA
              899  TGCGCA              
HincII        698  GTCAAC               GT[CT]^[GA]AC
             4209  GTCAAC              
HindII        698  GTCAAC               GT[CT]^[GA]AC
             4209  GTCAAC              
MaeII         845  ACGT                 A^CGT
             4127  ACGT                
PaeR7I        770  CTCGAG               C^TCGAG
             1489  CTCGAG              
PvuII        1792  CAGCTG               CAG^CTG
             2641  CAGCTG              
XhoI          770  CTCGAG               C^TCGAG
             1489  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcnI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PpuMI       PstI        
RsaI        SacI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfiI        SfoI        
SmaI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XmaCI       XmaI