##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AcyI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AviII BbeI BbrPI BcgI BglI
BlpI Bpu1102I BsaAI BsaHI BseRI
BsiWI BsmBI BsrBI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
CpoI DraII DraIII DrdI Eco47III
Eco81I EcoO109I FseI FspI HaeII
Hin1I KasI KpnI KspI MluI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflMI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SacI SacII SbfI SexAI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I StuI Van91I
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 1057 GTCGAC GT^[AC][GT]AC
AccII 3058 CGCG CG^CG
AccIII 5290 TCCGGA T^CCGGA
AclI 1721 AACGTT AA^CGTT
AflII 721 CTTAAG C^TTAAG
AgeI 135 ACCGGT A^CCGGT
AhdI 605 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 605 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3156 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3893 CTCGAG C^[CT]CG[GA]G
AvrII 3519 CCTAGG C^CTAGG
BalI 1816 TGGCCA TGG^CCA
BamHI 3856 GGATCC G^GATCC
BciVI 1518 GGATAC GTATCC(6/5)
BclI 3289 TGATCA T^GATCA
BcnI 3053 CCGGG CC^[GC]GG
BfrI 721 CTTAAG C^TTAAG
BlnI 3519 CCTAGG C^CTAGG
BseAI 5290 TCCGGA T^CCGGA
BsiEI 3050 CGGCCG CG[GA][CT]^CG
BsoBI 3893 CTCGAG C^[CT]CG[GA]G
Bsp1407I 5418 TGTACA T^GTACA
BspDI 2605 ATCGAT AT^CGAT
BspEI 5290 TCCGGA T^CCGGA
BspMI 730 GCAGGT ACCTGC(4/8)
BsrFI 135 ACCGGT [GA]^CCGG[CT]
BsrGI 5418 TGTACA T^GTACA
BssSI 593 CACGAG CACGAG(-5/-1)
BstUI 3058 CGCG CG^CG
BstXI 1508 CCACGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 3057 GCGC GCG^C
Cfr10I 135 ACCGGT [GA]^CCGG[CT]
ClaI 2605 ATCGAT AT^CGAT
DsaI 410 CCATGG C^C[GA][CT]GG
EagI 3050 CGGCCG C^GGCCG
Eam1105I 605 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3050 CGGCCG C^GGCCG
Eco52I 3050 CGGCCG C^GGCCG
EcoNI 13 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2258 GAATTC G^AATTC
FauI 1264 CCCGC CCCGC(4/6)
FbaI 3289 TGATCA T^GATCA
HgaI 630 GACGC GACGC(5/10)
HhaI 3057 GCGC GCG^C
HinP1I 3057 GCGC G^CGC
HpaI 4120 GTTAAC GTT^AAC
MluNI 1816 TGGCCA TGG^CCA
MroI 5290 TCCGGA T^CCGGA
MscI 1816 TGGCCA TGG^CCA
MvnI 3058 CGCG CG^CG
NciI 3053 CCGGG CC^[GC]GG
NcoI 410 CCATGG C^CATGG
PaeR7I 3893 CTCGAG C^TCGAG
PflFI 3156 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 135 ACCGGT A^CCGGT
Psp1406I 1721 AACGTT AA^CGTT
SalI 1057 GTCGAC G^TCGAC
SapI 112 GAAGAGC GCTCTTC(1/4)
ScaI 256 AGTACT AGT^ACT
SgrAI 134 CACCGGTG C[GA]^CCGG[CT]G
SspBI 5418 TGTACA T^GTACA
SwaI 2820 ATTTAAAT ATTT^AAAT
Tth111I 3156 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3893 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 4551 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
5382 CAGAAGCTG
AspHI 5027 GAGCAC G[AT]GC[AT]^C
5258 GTGCTC
AvaII 22 GGACC G^G[AT]CC
612 GGTCC
BanI 2487 GGTGCC G^G[CT][GA]CC
3202 GGTGCC
BbsI 3871 GAAGAC GAAGAC(2/6)
4036 GTCTTC
BbvCI 2527 CCTCAGC CCTCAGC(-5/-2)
156 GCTGAGG
BglII 799 AGATCT A^GATCT
3413 AGATCT
BpuAI 3871 GAAGAC GAAGAC(2/6)
4036 GTCTTC
BsaBI 1104 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4868 GATGCTCATC
BsaWI 135 ACCGGT [AT]^CCGG[AT]
5290 TCCGGA
BsiHKAI 5027 GAGCAC G[AT]GC[AT]^C
5258 GTGCTC
BspHI 1795 TCATGA T^CATGA
4193 TCATGA
BsrDI 42 CATTGC GCAATG(2/0)
1000 GCAATG
BstEII 985 GGTGACC G^GT[ACGT]ACC
2001 GGTGACC
BstPI 985 GGTGACC G^GT[ACGT]ACC
2001 GGTGACC
EaeI 1816 TGGCCA [CT]^GGCC[GA]
3050 CGGCCG
EcoO65I 985 GGTGACC G^GT[ACGT]ACC
2001 GGTGACC
EcoRV 1108 GATATC GAT^ATC
3524 GATATC
HincII 1057 GTCGAC GT[CT]^[GA]AC
4120 GTTAAC
HindII 1057 GTCGAC GT[CT]^[GA]AC
4120 GTTAAC
MamI 1104 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4868 GATGCTCATC
MfeI 1582 CAATTG C^AATTG
4199 CAATTG
MspA1I 2530 CAGCTG C[AC]G^C[GT]G
3191 CAGCTG
MunI 1582 CAATTG C^AATTG
4199 CAATTG
NdeI 271 CATATG CA^TATG
359 CATATG
PvuII 2530 CAGCTG CAG^CTG
3191 CAGCTG
RcaI 1795 TCATGA T^CATGA
4193 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI