##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BbeI        BciVI       
BlnI        BsaBI       BseAI       BspDI       BspEI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        DrdI        Eco47III    
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MroI        MunI        NarI        NheI        
NotI        NsiI        NspV        PacI        PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       Sse8387I    SspI        SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        2141  GACGTC               GACGT^C
AccI         1170  GTCTAC               GT^[AC][GT]AC
AflII        4546  CTTAAG               C^TTAAG
AhdI         1889  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3471  GTGCAC               G^TGCAC
AocI         2088  CCTGAGG              CC^T[ACGT]AGG
ApaLI        3471  GTGCAC               G^TGCAC
AspEI        1889  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        2847  CACGTG               CAC^GTG
BclI         2207  TGATCA               T^GATCA
BfrI         4546  CTTAAG               C^TTAAG
BglI         3000  GCCCCCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        2847  CACGTG               [CT]AC^GT[GA]
BsaWI         505  ACCGGA               [AT]^CCGG[AT]
BsiEI        1034  CGGCCG               CG[GA][CT]^CG
BsmBI         175  GAGACG               CGTCTC(1/5)
BsmI         2703  GAATGC               GAATGC(1/-1)
BsrDI        3166  CATTGC               GCAATG(2/0)
BsrFI        3129  GCCGGC               [GA]^CCGG[CT]
BstAPI       4273  GCAGTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2088  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       3129  GCCGGC               [GA]^CCGG[CT]
EagI         1034  CGGCCG               C^GGCCG
Eam1105I     1889  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1034  CGGCCG               C^GGCCG
Eco52I       1034  CGGCCG               C^GGCCG
Eco81I       2088  CCTGAGG              CC^T[ACGT]AGG
FbaI         2207  TGATCA               T^GATCA
MluI         3035  ACGCGT               A^CGCGT
NaeI         3129  GCCGGC               GCC^GGC
NdeI         3842  CATATG               CA^TATG
NgoMIV       3129  GCCGGC               G^CCGGC
NruI         3889  TCGCGA               TCG^CGA
PaeR7I       2168  CTCGAG               C^TCGAG
PmaCI        2847  CACGTG               CAC^GTG
PmlI         2847  CACGTG               CAC^GTG
SpeI         2923  ACTAGT               A^CTAGT
StuI           68  AGGCCT               AGG^CCT
XbaI         3704  TCTAGA               T^CTAGA
XhoI         2168  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          177  GACGCC               G[GA]^CG[CT]C
             2141  GACGTC              
AflIII       2848  ACGTGT               A^C[GA][CT]GT
             3035  ACGCGT              
BalI          223  TGGCCA               TGG^CCA
             1561  TGGCCA              
BanI         2665  GGCACC               G^G[CT][GA]CC
             4632  GGTGCC              
BcgI           34  CGATCACACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5313  CGACGACTTTGC        
BglII        4683  AGATCT               A^GATCT
             4714  AGATCT              
BlpI         1304  GCTCAGC              GC^T[ACGT]AGC
             1486  GCTCAGC             
Bpu1102I     1304  GCTCAGC              GC^T[ACGT]AGC
             1486  GCTCAGC             
BsaHI         177  GACGCC               G[GA]^CG[CT]C
             2141  GACGTC              
BsiWI         650  CGTACG               C^GTACG
             3023  CGTACG              
BspHI        1015  TCATGA               T^CATGA
             1231  TCATGA              
BsrBI        1049  CCGCTC               CCGCTC(-3/-3)
             1806  GAGCGG              
BssSI        3752  CACGAG               CACGAG(-5/-1)
             3688  CTCGTG              
BstEII        755  GGTCACC              G^GT[ACGT]ACC
             3835  GGTCACC             
BstPI         755  GGTCACC              G^GT[ACGT]ACC
             3835  GGTCACC             
BstXI        2846  CCACGTGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3244  CCACTGCGCTGG        
CelII        1304  GCTCAGC              GC^T[ACGT]AGC
             1486  GCTCAGC             
DraIII        889  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1613  CACTCGGTG           
EcoO65I       755  GGTCACC              G^GT[ACGT]ACC
             3835  GGTCACC             
EcoRI         423  GAATTC               G^AATTC
             2023  GAATTC              
Hin1I         177  GACGCC               G[GA]^CG[CT]C
             2141  GACGTC              
HincII       2955  GTCAAC               GT[CT]^[GA]AC
             3859  GTTGAC              
HindII       2955  GTCAAC               GT[CT]^[GA]AC
             3859  GTTGAC              
MluNI         223  TGGCCA               TGG^CCA
             1561  TGGCCA              
MscI          223  TGGCCA               TGG^CCA
             1561  TGGCCA              
RcaI         1015  TCATGA               T^CATGA
             1231  TCATGA              
SapI         2618  GAAGAGC              GCTCTTC(1/4)
              839  GCTCTTC             
ScaI         2906  AGTACT               AGT^ACT
             2920  AGTACT              
SmaI         2573  CCCGGG               CCC^GGG
             3487  CCCGGG              
SphI         3205  GCATGC               GCATG^C
             5177  GCATGC              
XmaCI        2573  CCCGGG               C^CCGGG
             3487  CCCGGG              
XmaI         2573  CCCGGG               C^CCGGG
             3487  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmnI