################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AccIII AclI AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BbeI BciVI BlnI BsaBI BseAI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MroI MunI NarI NheI NotI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 2141 GACGTC GACGT^C AccI 1170 GTCTAC GT^[AC][GT]AC AflII 4546 CTTAAG C^TTAAG AhdI 1889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3471 GTGCAC G^TGCAC AocI 2088 CCTGAGG CC^T[ACGT]AGG ApaLI 3471 GTGCAC G^TGCAC AspEI 1889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 2847 CACGTG CAC^GTG BclI 2207 TGATCA T^GATCA BfrI 4546 CTTAAG C^TTAAG BglI 3000 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2847 CACGTG [CT]AC^GT[GA] BsaWI 505 ACCGGA [AT]^CCGG[AT] BsiEI 1034 CGGCCG CG[GA][CT]^CG BsmBI 175 GAGACG CGTCTC(1/5) BsmI 2703 GAATGC GAATGC(1/-1) BsrDI 3166 CATTGC GCAATG(2/0) BsrFI 3129 GCCGGC [GA]^CCGG[CT] BstAPI 4273 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2088 CCTGAGG CC^T[ACGT]AGG Cfr10I 3129 GCCGGC [GA]^CCGG[CT] EagI 1034 CGGCCG C^GGCCG Eam1105I 1889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1034 CGGCCG C^GGCCG Eco52I 1034 CGGCCG C^GGCCG Eco81I 2088 CCTGAGG CC^T[ACGT]AGG FbaI 2207 TGATCA T^GATCA MluI 3035 ACGCGT A^CGCGT NaeI 3129 GCCGGC GCC^GGC NdeI 3842 CATATG CA^TATG NgoMIV 3129 GCCGGC G^CCGGC NruI 3889 TCGCGA TCG^CGA PaeR7I 2168 CTCGAG C^TCGAG PmaCI 2847 CACGTG CAC^GTG PmlI 2847 CACGTG CAC^GTG SpeI 2923 ACTAGT A^CTAGT StuI 68 AGGCCT AGG^CCT XbaI 3704 TCTAGA T^CTAGA XhoI 2168 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 177 GACGCC G[GA]^CG[CT]C 2141 GACGTC AflIII 2848 ACGTGT A^C[GA][CT]GT 3035 ACGCGT BalI 223 TGGCCA TGG^CCA 1561 TGGCCA BanI 2665 GGCACC G^G[CT][GA]CC 4632 GGTGCC BcgI 34 CGATCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5313 CGACGACTTTGC BglII 4683 AGATCT A^GATCT 4714 AGATCT BlpI 1304 GCTCAGC GC^T[ACGT]AGC 1486 GCTCAGC Bpu1102I 1304 GCTCAGC GC^T[ACGT]AGC 1486 GCTCAGC BsaHI 177 GACGCC G[GA]^CG[CT]C 2141 GACGTC BsiWI 650 CGTACG C^GTACG 3023 CGTACG BspHI 1015 TCATGA T^CATGA 1231 TCATGA BsrBI 1049 CCGCTC CCGCTC(-3/-3) 1806 GAGCGG BssSI 3752 CACGAG CACGAG(-5/-1) 3688 CTCGTG BstEII 755 GGTCACC G^GT[ACGT]ACC 3835 GGTCACC BstPI 755 GGTCACC G^GT[ACGT]ACC 3835 GGTCACC BstXI 2846 CCACGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3244 CCACTGCGCTGG CelII 1304 GCTCAGC GC^T[ACGT]AGC 1486 GCTCAGC DraIII 889 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG 1613 CACTCGGTG EcoO65I 755 GGTCACC G^GT[ACGT]ACC 3835 GGTCACC EcoRI 423 GAATTC G^AATTC 2023 GAATTC Hin1I 177 GACGCC G[GA]^CG[CT]C 2141 GACGTC HincII 2955 GTCAAC GT[CT]^[GA]AC 3859 GTTGAC HindII 2955 GTCAAC GT[CT]^[GA]AC 3859 GTTGAC MluNI 223 TGGCCA TGG^CCA 1561 TGGCCA MscI 223 TGGCCA TGG^CCA 1561 TGGCCA RcaI 1015 TCATGA T^CATGA 1231 TCATGA SapI 2618 GAAGAGC GCTCTTC(1/4) 839 GCTCTTC ScaI 2906 AGTACT AGT^ACT 2920 AGTACT SmaI 2573 CCCGGG CCC^GGG 3487 CCCGGG SphI 3205 GCATGC GCATG^C 5177 GCATGC XmaCI 2573 CCCGGG C^CCGGG 3487 CCCGGG XmaI 2573 CCCGGG C^CCGGG 3487 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI