################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccII AclI AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BcnI BlnI BlpI Bpu1102I BsaAI BsaI BsiEI BsiWI BspDI BspHI BspLU11I BsrFI BssHII BstUI CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoNI FseI FspI KasI KpnI KspI MluI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 2467 TCCGGA T^CCGGA AcyI 1896 GACGCC G[GA]^CG[CT]C AflII 4731 CTTAAG C^TTAAG AflIII 3500 ACACGT A^C[GA][CT]GT AocI 4184 CCTAAGG CC^T[ACGT]AGG Aor51HI 1106 AGCGCT AGC^GCT ApaI 2649 GGGCCC GGGCC^C BamHI 2205 GGATCC G^GATCC BfrI 4731 CTTAAG C^TTAAG BglI 1864 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3675 AGATCT A^GATCT BsaHI 1896 GACGCC G[GA]^CG[CT]C BseAI 2467 TCCGGA T^CCGGA BsgI 2612 GTGCAG GTGCAG(16/14) BspEI 2467 TCCGGA T^CCGGA BspMI 3450 ACCTGC ACCTGC(4/8) BsrBI 1972 CCGCTC CCGCTC(-3/-3) BssSI 4150 CTCGTG CACGAG(-5/-1) BstEII 2877 GGTCACC G^GT[ACGT]ACC BstPI 2877 GGTCACC G^GT[ACGT]ACC Bsu36I 4184 CCTAAGG CC^T[ACGT]AGG CfoI 1107 GCGC GCG^C DraIII 260 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1106 AGCGCT AGC^GCT Eco81I 4184 CCTAAGG CC^T[ACGT]AGG EcoO65I 2877 GGTCACC G^GT[ACGT]ACC FauI 2083 CCCGC CCCGC(4/6) HaeII 1106 AGCGCT [GA]GCGC^[CT] HgaI 1896 GACGC GACGC(5/10) HhaI 1107 GCGC GCG^C Hin1I 1896 GACGCC G[GA]^CG[CT]C HinP1I 1107 GCGC G^CGC HpaI 1215 GTTAAC GTT^AAC MroI 2467 TCCGGA T^CCGGA NcoI 1865 CCATGG C^CATGG PaeR7I 2474 CTCGAG C^TCGAG PpuMI 3918 AGGTCCT [GA]G^G[AT]CC[CT] SexAI 2873 ACCTGGT A^CC[AT]GGT XhoI 2474 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AseI 2211 ATTAAT AT^TAAT 2797 ATTAAT AsnI 2211 ATTAAT AT^TAAT 2797 ATTAAT AvaI 1010 CTCGGG C^[CT]CG[GA]G 2474 CTCGAG BanI 2513 GGCACC G^G[CT][GA]CC 2556 GGCACC BsaWI 2467 TCCGGA [AT]^CCGG[AT] 2881 ACCGGA BseRI 1070 CTCCTC GAGGAG(10/8) 2153 GAGGAG BsmBI 480 GAGACG CGTCTC(1/5) 641 GAGACG BsmI 108 GAATGC GAATGC(1/-1) 3653 GCATTC BsoBI 1010 CTCGGG C^[CT]CG[GA]G 2474 CTCGAG Bst1107I 2270 GTATAC GTA^TAC 4025 GTATAC BstAPI 112 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1777 GCAGTTCGTGC BstBI 1135 TTCGAA TT^CGAA 1415 TTCGAA BstXI 2195 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4010 CCACTGGCTTGG BstZ17I 2270 GTATAC GTA^TAC 4025 GTATAC DrdI 172 GACCATATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4312 GACTGTATCGTC EcoRI 4534 GAATTC G^AATTC 4826 GAATTC EcoRV 1056 GATATC GAT^ATC 2730 GATATC EcoT22I 497 ATGCAT ATGCA^T 3525 ATGCAT HindIII 3289 AAGCTT A^AGCTT 3436 AAGCTT MaeII 900 ACGT A^CGT 3502 ACGT MfeI 427 CAATTG C^AATTG 1283 CAATTG MspA1I 358 CAGCTG C[AC]G^C[GT]G 3274 CAGCTG MunI 427 CAATTG C^AATTG 1283 CAATTG NsiI 497 ATGCAT ATGCA^T 3525 ATGCAT NspV 1135 TTCGAA TT^CGAA 1415 TTCGAA PflMI 2868 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3979 CCAGCACCTGG PshBI 2211 ATTAAT AT^TAAT 2797 ATTAAT PvuII 358 CAGCTG CAG^CTG 3274 CAGCTG SfuI 1135 TTCGAA TT^CGAA 1415 TTCGAA SpeI 1513 ACTAGT A^CTAGT 4515 ACTAGT Van91I 2868 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3979 CCAGCACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanII BbsI BbvI BciVI BclI BfaI BmyI BpmI BpuAI BsaBI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI