##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AgeI        
AhdI        Alw44I      ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BcnI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaI        BsiEI       
BsiWI       BspDI       BspHI       BspLU11I    BsrFI       
BssHII      BstUI       CelII       Cfr10I      ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       FseI        FspI        KasI        KpnI        
KspI        MluI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SwaI        Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccIII       2467  TCCGGA               T^CCGGA
AcyI         1896  GACGCC               G[GA]^CG[CT]C
AflII        4731  CTTAAG               C^TTAAG
AflIII       3500  ACACGT               A^C[GA][CT]GT
AocI         4184  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      1106  AGCGCT               AGC^GCT
ApaI         2649  GGGCCC               GGGCC^C
BamHI        2205  GGATCC               G^GATCC
BfrI         4731  CTTAAG               C^TTAAG
BglI         1864  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3675  AGATCT               A^GATCT
BsaHI        1896  GACGCC               G[GA]^CG[CT]C
BseAI        2467  TCCGGA               T^CCGGA
BsgI         2612  GTGCAG               GTGCAG(16/14)
BspEI        2467  TCCGGA               T^CCGGA
BspMI        3450  ACCTGC               ACCTGC(4/8)
BsrBI        1972  CCGCTC               CCGCTC(-3/-3)
BssSI        4150  CTCGTG               CACGAG(-5/-1)
BstEII       2877  GGTCACC              G^GT[ACGT]ACC
BstPI        2877  GGTCACC              G^GT[ACGT]ACC
Bsu36I       4184  CCTAAGG              CC^T[ACGT]AGG
CfoI         1107  GCGC                 GCG^C
DraIII        260  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1106  AGCGCT               AGC^GCT
Eco81I       4184  CCTAAGG              CC^T[ACGT]AGG
EcoO65I      2877  GGTCACC              G^GT[ACGT]ACC
FauI         2083  CCCGC                CCCGC(4/6)
HaeII        1106  AGCGCT               [GA]GCGC^[CT]
HgaI         1896  GACGC                GACGC(5/10)
HhaI         1107  GCGC                 GCG^C
Hin1I        1896  GACGCC               G[GA]^CG[CT]C
HinP1I       1107  GCGC                 G^CGC
HpaI         1215  GTTAAC               GTT^AAC
MroI         2467  TCCGGA               T^CCGGA
NcoI         1865  CCATGG               C^CATGG
PaeR7I       2474  CTCGAG               C^TCGAG
PpuMI        3918  AGGTCCT              [GA]G^G[AT]CC[CT]
SexAI        2873  ACCTGGT              A^CC[AT]GGT
XhoI         2474  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AseI         2211  ATTAAT               AT^TAAT
             2797  ATTAAT              
AsnI         2211  ATTAAT               AT^TAAT
             2797  ATTAAT              
AvaI         1010  CTCGGG               C^[CT]CG[GA]G
             2474  CTCGAG              
BanI         2513  GGCACC               G^G[CT][GA]CC
             2556  GGCACC              
BsaWI        2467  TCCGGA               [AT]^CCGG[AT]
             2881  ACCGGA              
BseRI        1070  CTCCTC               GAGGAG(10/8)
             2153  GAGGAG              
BsmBI         480  GAGACG               CGTCTC(1/5)
              641  GAGACG              
BsmI          108  GAATGC               GAATGC(1/-1)
             3653  GCATTC              
BsoBI        1010  CTCGGG               C^[CT]CG[GA]G
             2474  CTCGAG              
Bst1107I     2270  GTATAC               GTA^TAC
             4025  GTATAC              
BstAPI        112  GCAGGACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1777  GCAGTTCGTGC         
BstBI        1135  TTCGAA               TT^CGAA
             1415  TTCGAA              
BstXI        2195  CCATTGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4010  CCACTGGCTTGG        
BstZ17I      2270  GTATAC               GTA^TAC
             4025  GTATAC              
DrdI          172  GACCATATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4312  GACTGTATCGTC        
EcoRI        4534  GAATTC               G^AATTC
             4826  GAATTC              
EcoRV        1056  GATATC               GAT^ATC
             2730  GATATC              
EcoT22I       497  ATGCAT               ATGCA^T
             3525  ATGCAT              
HindIII      3289  AAGCTT               A^AGCTT
             3436  AAGCTT              
MaeII         900  ACGT                 A^CGT
             3502  ACGT                
MfeI          427  CAATTG               C^AATTG
             1283  CAATTG              
MspA1I        358  CAGCTG               C[AC]G^C[GT]G
             3274  CAGCTG              
MunI          427  CAATTG               C^AATTG
             1283  CAATTG              
NsiI          497  ATGCAT               ATGCA^T
             3525  ATGCAT              
NspV         1135  TTCGAA               TT^CGAA
             1415  TTCGAA              
PflMI        2868  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3979  CCAGCACCTGG         
PshBI        2211  ATTAAT               AT^TAAT
             2797  ATTAAT              
PvuII         358  CAGCTG               CAG^CTG
             3274  CAGCTG              
SfuI         1135  TTCGAA               TT^CGAA
             1415  TTCGAA              
SpeI         1513  ACTAGT               A^CTAGT
             4515  ACTAGT              
Van91I       2868  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3979  CCAGCACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BanII       BbsI        BbvI        
BciVI       BclI        BfaI        BmyI        BpmI        
BpuAI       BsaBI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BsrDI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmnI