##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BcnI BlnI
BlpI Bpu1102I BsaAI BsaI BsiEI
BsiWI BspDI BspHI BspLU11I BsrFI
BssHII BstUI CelII Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoNI FseI FspI KasI KpnI
KspI MluI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 2467 TCCGGA T^CCGGA
AcyI 1896 GACGCC G[GA]^CG[CT]C
AflII 4731 CTTAAG C^TTAAG
AflIII 3500 ACACGT A^C[GA][CT]GT
AocI 4184 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1106 AGCGCT AGC^GCT
ApaI 2649 GGGCCC GGGCC^C
BamHI 2205 GGATCC G^GATCC
BfrI 4731 CTTAAG C^TTAAG
BglI 1864 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3675 AGATCT A^GATCT
BsaHI 1896 GACGCC G[GA]^CG[CT]C
BseAI 2467 TCCGGA T^CCGGA
BsgI 2612 GTGCAG GTGCAG(16/14)
BspEI 2467 TCCGGA T^CCGGA
BspMI 3450 ACCTGC ACCTGC(4/8)
BsrBI 1972 CCGCTC CCGCTC(-3/-3)
BssSI 4150 CTCGTG CACGAG(-5/-1)
BstEII 2877 GGTCACC G^GT[ACGT]ACC
BstPI 2877 GGTCACC G^GT[ACGT]ACC
Bsu36I 4184 CCTAAGG CC^T[ACGT]AGG
CfoI 1107 GCGC GCG^C
DraIII 260 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1106 AGCGCT AGC^GCT
Eco81I 4184 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2877 GGTCACC G^GT[ACGT]ACC
FauI 2083 CCCGC CCCGC(4/6)
HaeII 1106 AGCGCT [GA]GCGC^[CT]
HgaI 1896 GACGC GACGC(5/10)
HhaI 1107 GCGC GCG^C
Hin1I 1896 GACGCC G[GA]^CG[CT]C
HinP1I 1107 GCGC G^CGC
HpaI 1215 GTTAAC GTT^AAC
MroI 2467 TCCGGA T^CCGGA
NcoI 1865 CCATGG C^CATGG
PaeR7I 2474 CTCGAG C^TCGAG
PpuMI 3918 AGGTCCT [GA]G^G[AT]CC[CT]
SexAI 2873 ACCTGGT A^CC[AT]GGT
XhoI 2474 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AseI 2211 ATTAAT AT^TAAT
2797 ATTAAT
AsnI 2211 ATTAAT AT^TAAT
2797 ATTAAT
AvaI 1010 CTCGGG C^[CT]CG[GA]G
2474 CTCGAG
BanI 2513 GGCACC G^G[CT][GA]CC
2556 GGCACC
BsaWI 2467 TCCGGA [AT]^CCGG[AT]
2881 ACCGGA
BseRI 1070 CTCCTC GAGGAG(10/8)
2153 GAGGAG
BsmBI 480 GAGACG CGTCTC(1/5)
641 GAGACG
BsmI 108 GAATGC GAATGC(1/-1)
3653 GCATTC
BsoBI 1010 CTCGGG C^[CT]CG[GA]G
2474 CTCGAG
Bst1107I 2270 GTATAC GTA^TAC
4025 GTATAC
BstAPI 112 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1777 GCAGTTCGTGC
BstBI 1135 TTCGAA TT^CGAA
1415 TTCGAA
BstXI 2195 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4010 CCACTGGCTTGG
BstZ17I 2270 GTATAC GTA^TAC
4025 GTATAC
DrdI 172 GACCATATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4312 GACTGTATCGTC
EcoRI 4534 GAATTC G^AATTC
4826 GAATTC
EcoRV 1056 GATATC GAT^ATC
2730 GATATC
EcoT22I 497 ATGCAT ATGCA^T
3525 ATGCAT
HindIII 3289 AAGCTT A^AGCTT
3436 AAGCTT
MaeII 900 ACGT A^CGT
3502 ACGT
MfeI 427 CAATTG C^AATTG
1283 CAATTG
MspA1I 358 CAGCTG C[AC]G^C[GT]G
3274 CAGCTG
MunI 427 CAATTG C^AATTG
1283 CAATTG
NsiI 497 ATGCAT ATGCA^T
3525 ATGCAT
NspV 1135 TTCGAA TT^CGAA
1415 TTCGAA
PflMI 2868 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3979 CCAGCACCTGG
PshBI 2211 ATTAAT AT^TAAT
2797 ATTAAT
PvuII 358 CAGCTG CAG^CTG
3274 CAGCTG
SfuI 1135 TTCGAA TT^CGAA
1415 TTCGAA
SpeI 1513 ACTAGT A^CTAGT
4515 ACTAGT
Van91I 2868 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3979 CCAGCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BciVI BclI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI