##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AviII AvrII
BbrPI BfrI BglI BlnI BsaAI
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BssHII BssSI Bst1107I BstZ17I
Bsu36I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NheI NotI NruI PaeR7I PflMI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SapI SbfI
SexAI SfiI SgrAI SnaBI SphI
Sse8387I SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 421 GTCTAC GT^[AC][GT]AC
AcyI 143 GGCGCC G[GA]^CG[CT]C
AflIII 2455 ACATGT A^C[GA][CT]GT
AgeI 2383 ACCGGT A^CCGGT
AspI 2432 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 28 GGATCC G^GATCC
BbeI 143 GGCGCC GGCGC^C
BbvCI 2131 CCTCAGC CCTCAGC(-5/-2)
BcgI 1114 GCACCAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1188 TGATCA T^GATCA
BlpI 1154 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1154 GCTAAGC GC^T[ACGT]AGC
BsaHI 143 GGCGCC G[GA]^CG[CT]C
BsaWI 2383 ACCGGT [AT]^CCGG[AT]
BsiEI 850 CGACCG CG[GA][CT]^CG
Bsp1407I 3430 TGTACA T^GTACA
BspLU11I 2455 ACATGT A^CATGT
BsrGI 3430 TGTACA T^GTACA
BstAPI 1375 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4561 TTCGAA TT^CGAA
CelII 1154 GCTAAGC GC^T[ACGT]AGC
EcoRI 2567 GAATTC G^AATTC
FbaI 1188 TGATCA T^GATCA
HaeII 143 GGCGCC [GA]GCGC^[CT]
HgaI 829 GACGC GACGC(5/10)
Hin1I 143 GGCGCC G[GA]^CG[CT]C
HincII 2682 GTTGAC GT[CT]^[GA]AC
HindII 2682 GTTGAC GT[CT]^[GA]AC
KasI 143 GGCGCC G^GCGCC
KspI 233 CCGCGG CCGC^GG
MaeII 693 ACGT A^CGT
NaeI 1356 GCCGGC GCC^GGC
NarI 143 GGCGCC GG^CGCC
NgoMIV 1356 GCCGGC G^CCGGC
NspV 4561 TTCGAA TT^CGAA
PacI 2606 TTAATTAA TTAAT^TAA
PflFI 2432 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2383 ACCGGT A^CCGGT
SacII 233 CCGCGG CCGC^GG
ScaI 3121 AGTACT AGT^ACT
SfoI 143 GGCGCC GGC^GCC
SfuI 4561 TTCGAA TT^CGAA
SpeI 4969 ACTAGT A^CTAGT
SspBI 3430 TGTACA T^GTACA
Tth111I 2432 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3754 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 52 GGGCCC GGGCC^C
3843 GGGCCC
Asp700I 317 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4499 GAAACATTTC
BciVI 2639 GGATAC GTATCC(6/5)
1773 GTATCC
BglII 3086 AGATCT A^GATCT
3619 AGATCT
BsrBI 72 GAGCGG CCGCTC(-3/-3)
102 GAGCGG
BsrDI 2687 CATTGC GCAATG(2/0)
4341 CATTGC
BstEII 187 GGTCACC G^GT[ACGT]ACC
1102 GGTGACC
BstPI 187 GGTCACC G^GT[ACGT]ACC
1102 GGTGACC
DraI 2481 TTTAAA TTT^AAA
4424 TTTAAA
DraIII 866 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4572 CACCTTGTG
DrdI 686 GACTGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5068 GACATTGTAGTC
EcoO65I 187 GGTCACC G^GT[ACGT]ACC
1102 GGTGACC
EcoT22I 1596 ATGCAT ATGCA^T
5060 ATGCAT
NdeI 4800 CATATG CA^TATG
5079 CATATG
NsiI 1596 ATGCAT ATGCA^T
5060 ATGCAT
SmaI 32 CCCGGG CCC^GGG
60 CCCGGG
StuI 1221 AGGCCT AGG^CCT
3135 AGGCCT
XmaCI 32 CCCGGG C^CCGGG
60 CCCGGG
XmaI 32 CCCGGG C^CCGGG
60 CCCGGG
XmnI 317 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4499 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII