##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AhdI        Aor51HI     ApaI        AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BlnI        BsaAI       BsaI        BsiEI       BsiWI       
BspDI       BspHI       BspLU11I    BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       FbaI        FseI        FspI        HindIII     
KasI        KpnI        MluI        NarI        NdeI        
NheI        NotI        NruI        NspV        PflFI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SnaBI       Sse8387I    
SspI        StuI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        154  TCCGGA               T^CCGGA
AcyI          194  GACGCC               G[GA]^CG[CT]C
AseI         3966  ATTAAT               AT^TAAT
AsnI         3966  ATTAAT               AT^TAAT
BalI          304  TGGCCA               TGG^CCA
BbvCI        1810  CCTCAGC              CCTCAGC(-5/-2)
BglI         5349  GCCATAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          994  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      994  GCTAAGC              GC^T[ACGT]AGC
BsaBI        4183  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         194  GACGCC               G[GA]^CG[CT]C
BseAI         154  TCCGGA               T^CCGGA
BspEI         154  TCCGGA               T^CCGGA
BsrBI          30  GAGCGG               CCGCTC(-3/-3)
BssSI        1111  CACGAG               CACGAG(-5/-1)
CelII         994  GCTAAGC              GC^T[ACGT]AGC
DraIII       2166  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I      5390  ATGCAT               ATGCA^T
HaeII        1763  AGCGCC               [GA]GCGC^[CT]
HgaI          194  GACGC                GACGC(5/10)
Hin1I         194  GACGCC               G[GA]^CG[CT]C
HpaI         2489  GTTAAC               GTT^AAC
KspI         2217  CCGCGG               CCGC^GG
MamI         4183  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4148  CAATTG               C^AATTG
MluNI         304  TGGCCA               TGG^CCA
MroI          154  TCCGGA               T^CCGGA
MscI          304  TGGCCA               TGG^CCA
MunI         4148  CAATTG               C^AATTG
NaeI         1141  GCCGGC               GCC^GGC
NgoMIV       1141  GCCGGC               G^CCGGC
NsiI         5390  ATGCAT               ATGCA^T
NspI         2993  GCATGC               [GA]CATG^[CT]
PacI          758  TTAATTAA             TTAAT^TAA
PshAI        2261  GACTCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3966  ATTAAT               AT^TAAT
SacI         1174  GAGCTC               GAGCT^C
SacII        2217  CCGCGG               CCGC^GG
SexAI         357  ACCAGGT              A^CC[AT]GGT
SgrAI        2472  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         1068  CCCGGG               CCC^GGG
SpeI          466  ACTAGT               A^CTAGT
SphI         2993  GCATGC               GCATG^C
XcmI         3356  CCAGAACAGGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1068  CCCGGG               C^CCGGG
XmaI         1068  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          417  GTAGAC               GT^[AC][GT]AC
             3076  GTCTAC              
AccII        2218  CGCG                 CG^CG
             2242  CGCG                
AgeI         1318  ACCGGT               A^CCGGT
             2473  ACCGGT              
AocI         2187  CCTCAGG              CC^T[ACGT]AGG
             3133  CCTCAGG             
BamHI         940  GGATCC               G^GATCC
             3127  GGATCC              
BglII         807  AGATCT               A^GATCT
             5328  AGATCT              
BsmBI         742  CGTCTC               CGTCTC(1/5)
             2040  CGTCTC              
Bsp1407I     4667  TGTACA               T^GTACA
             4693  TGTACA              
BspMI         977  ACCTGC               ACCTGC(4/8)
             2509  GCAGGT              
BsrGI        4667  TGTACA               T^GTACA
             4693  TGTACA              
BstEII       1328  GGTGACC              G^GT[ACGT]ACC
             3572  GGTGACC             
BstPI        1328  GGTGACC              G^GT[ACGT]ACC
             3572  GGTGACC             
BstUI        2218  CGCG                 CG^CG
             2242  CGCG                
BstXI        1422  CCAGACCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4143  CCACACAATTGG        
Bsu36I       2187  CCTCAGG              CC^T[ACGT]AGG
             3133  CCTCAGG             
Eco81I       2187  CCTCAGG              CC^T[ACGT]AGG
             3133  CCTCAGG             
EcoO65I      1328  GGTGACC              G^GT[ACGT]ACC
             3572  GGTGACC             
FauI         2216  CCCGC                CCCGC(4/6)
             2455  CCCGC               
HincII       2489  GTTAAC               GT[CT]^[GA]AC
             5111  GTCAAC              
HindII       2489  GTTAAC               GT[CT]^[GA]AC
             5111  GTCAAC              
MaeII        3074  ACGT                 A^CGT
             4361  ACGT                
MvnI         2218  CGCG                 CG^CG
             2242  CGCG                
PaeR7I       1687  CTCGAG               C^TCGAG
             2386  CTCGAG              
PinAI        1318  ACCGGT               A^CCGGT
             2473  ACCGGT              
PvuII        3313  CAGCTG               CAG^CTG
             3580  CAGCTG              
SspBI        4667  TGTACA               T^GTACA
             4693  TGTACA              
XhoI         1687  CTCGAG               C^TCGAG
             2386  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        AlwNI       ApaLI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI