##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AocI AscI AseI AsnI
Asp718I AvrII BamHI BciVI BclI
BfrI BglII BlnI BsiWI BsmBI
BsmI BspDI BspHI BspLU11I BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI DrdI
Eco81I EcoO65I EcoRV EcoT22I FbaI
HpaI KpnI MfeI MflI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SalI ScaI SfuI SgrAI SnaBI
SpeI SwaI XbaI XhoII
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1082 TCCGGA T^CCGGA
AflIII 4080 ACGTGT A^C[GA][CT]GT
AhdI 3636 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 3458 GATCC GGATC(4/5)
Aor51HI 4011 AGCGCT AGC^GCT
AspEI 3636 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3749 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 918 GCTGAGG CCTCAGC(-5/-2)
BcgI 2354 GCACAGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 3458 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1082 TCCGGA [AT]^CCGG[AT]
BseAI 1082 TCCGGA T^CCGGA
Bsp1407I 4651 TGTACA T^GTACA
BspEI 1082 TCCGGA T^CCGGA
BsrGI 4651 TGTACA T^GTACA
CpoI 4175 CGGACCG CG^G[AT]CCG
Eam1105I 3636 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4011 AGCGCT AGC^GCT
EcoRI 1852 GAATTC G^AATTC
FseI 4218 GGCCGGCC GGCCGG^CC
HincII 4712 GTCAAC GT[CT]^[GA]AC
HindII 4712 GTCAAC GT[CT]^[GA]AC
HindIII 1191 AAGCTT A^AGCTT
MamI 3458 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1082 TCCGGA T^CCGGA
PaeR7I 2140 CTCGAG C^TCGAG
PflFI 3749 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 4175 CGGACCG CG^G[AT]CCG
SexAI 4515 ACCAGGT A^CC[AT]GGT
SspBI 4651 TGTACA T^GTACA
SspI 4825 AATATT AAT^ATT
Tth111I 3749 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2140 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 3368 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3689 GAAGGACTTC
BssHII 3704 GCGCGC G^CGCGC
3706 GCGCGC
DraI 4702 TTTAAA TTT^AAA
4769 TTTAAA
DraIII 4514 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4912 CACCAAGTG
EagI 3494 CGGCCG C^GGCCG
3518 CGGCCG
EclXI 3494 CGGCCG C^GGCCG
3518 CGGCCG
Eco52I 3494 CGGCCG C^GGCCG
3518 CGGCCG
MaeIII 521 GTGAC ^GT[ACGT]AC
2202 GTCAC
NcoI 191 CCATGG C^CATGG
3975 CCATGG
SapI 1272 GAAGAGC GCTCTTC(1/4)
3422 GCTCTTC
SbfI 208 CCTGCAGG CCTGCA^GG
4017 CCTGCAGG
SphI 4004 GCATGC GCATG^C
4074 GCATGC
Sse8387I 208 CCTGCAGG CCTGCA^GG
4017 CCTGCAGG
Tsp45I 521 GTGAC ^GT[GC]AC
2202 GTCAC
XmnI 3368 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3689 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwNI ApaI ApaLI
ApoI AspHI AvaI AvaII AviII
BalI BanI BanII BbeI BbrPI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI