##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AscI        
AseI        AsnI        AviII       BcgI        BciVI       
BclI        BfrI        BsaBI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstZ17I     ClaI        CpoI        DraI        
DrdI        EagI        EclXI       Eco52I      EcoRV       
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
ScaI        SfiI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       2043  GGTACC               G^GTACC
AccI         1198  GTCTAC               GT^[AC][GT]AC
AccIII       4326  TCCGGA               T^CCGGA
AcsI         2453  GAATTC               [GA]^AATT[CT]
AhdI         3972  GACGGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1673  CCTGAGG              CC^T[ACGT]AGG
ApoI         2453  GAATTC               [GA]^AATT[CT]
Asp700I      2431  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2043  GGTACC               G^GTACC
AspEI        3972  GACGGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI           27  GGCGCC               GGCGC^C
BbrPI        2840  CACGTG               CAC^GTG
BsaAI        2840  CACGTG               [CT]AC^GT[GA]
BseAI        4326  TCCGGA               T^CCGGA
BsmBI        1518  GAGACG               CGTCTC(1/5)
BsmI         1643  GCATTC               GAATGC(1/-1)
BspEI        4326  TCCGGA               T^CCGGA
BsrDI        4343  CATTGC               GCAATG(2/0)
BssHII        292  GCGCGC               G^CGCGC
BstBI        1050  TTCGAA               TT^CGAA
Bsu36I       1673  CCTGAGG              CC^T[ACGT]AGG
Eam1105I     3972  GACGGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1673  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2453  GAATTC               G^AATTC
KasI           27  GGCGCC               G^GCGCC
KpnI         2043  GGTACC               GGTAC^C
KspI          364  CCGCGG               CCGC^GG
MroI         4326  TCCGGA               T^CCGGA
NarI           27  GGCGCC               GG^CGCC
NspI          707  ACATGC               [GA]CATG^[CT]
NspV         1050  TTCGAA               TT^CGAA
PmaCI        2840  CACGTG               CAC^GTG
PmlI         2840  CACGTG               CAC^GTG
PshAI         991  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         364  CCGCGG               CCGC^GG
SbfI         2956  CCTGCAGG             CCTGCA^GG
SfoI           27  GGCGCC               GGC^GCC
SfuI         1050  TTCGAA               TT^CGAA
Sse8387I     2956  CCTGCAGG             CCTGCA^GG
SspI         4687  AATATT               AAT^ATT
XmnI         2431  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII       2565  ACGTGT               A^C[GA][CT]GT
             2841  ACGTGT              
Alw44I       2820  GTGCAC               G^TGCAC
             2941  GTGCAC              
Aor51HI       314  AGCGCT               AGC^GCT
             4079  AGCGCT              
ApaLI        2820  GTGCAC               G^TGCAC
             2941  GTGCAC              
AvrII         622  CCTAGG               C^CTAGG
             3115  CCTAGG              
BglII        1337  AGATCT               A^GATCT
             2499  AGATCT              
BlnI          622  CCTAGG               C^CTAGG
             3115  CCTAGG              
BsaWI        2214  ACCGGA               [AT]^CCGG[AT]
             4326  TCCGGA              
BssSI        3476  CACGAG               CACGAG(-5/-1)
             3677  CACGAG              
DraIII       1223  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2651  CACACGGTG           
Eco47III      314  AGCGCT               AGC^GCT
             4079  AGCGCT              
EcoT22I      1641  ATGCAT               ATGCA^T
             4310  ATGCAT              
NaeI         2891  GCCGGC               GCC^GGC
             2922  GCCGGC              
NcoI         3934  CCATGG               C^CATGG
             4174  CCATGG              
NgoMIV       2891  GCCGGC               G^CCGGC
             2922  GCCGGC              
NsiI         1641  ATGCAT               ATGCA^T
             4310  ATGCAT              
SapI          376  GAAGAGC              GCTCTTC(1/4)
              526  GCTCTTC             
SexAI         938  ACCTGGT              A^CC[AT]GGT
             1224  ACCTGGT             
Tsp509I      2454  AATT                 ^AATT
             4681  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AspI        
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI