################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccI AccII AccIII AflII AgeI Aor51HI ApaI AscI Asp700I AviII BamHI BbeI BbrPI BcgI BclI BfrI BglI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstUI BstZ17I ClaI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MamI MluI MroI MvnI NarI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PvuI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 4318 AACGTT AA^CGTT AcyI 3854 GGCGTC G[GA]^CG[CT]C Alw44I 5538 GTGCAC G^TGCAC ApaLI 5538 GTGCAC G^TGCAC AspI 5621 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 4248 GGATAC GTATCC(6/5) BlpI 230 GCTTAGC GC^T[ACGT]AGC Bpu1102I 230 GCTTAGC GC^T[ACGT]AGC BsaHI 3854 GGCGTC G[GA]^CG[CT]C BsmBI 1525 GAGACG CGTCTC(1/5) Bsp1407I 4832 TGTACA T^GTACA BspLU11I 3309 ACATGT A^CATGT BsrFI 4043 GCCGGC [GA]^CCGG[CT] BsrGI 4832 TGTACA T^GTACA BssSI 4359 CACGAG CACGAG(-5/-1) BstEII 2517 GGTCACC G^GT[ACGT]ACC BstPI 2517 GGTCACC G^GT[ACGT]ACC CelII 230 GCTTAGC GC^T[ACGT]AGC Cfr10I 4043 GCCGGC [GA]^CCGG[CT] CpoI 3547 CGGTCCG CG^G[AT]CCG EcoO65I 2517 GGTCACC G^GT[ACGT]ACC HaeII 3114 GGCGCT [GA]GCGC^[CT] Hin1I 3854 GGCGTC G[GA]^CG[CT]C HincII 3922 GTTGAC GT[CT]^[GA]AC HindII 3922 GTTGAC GT[CT]^[GA]AC NaeI 4043 GCCGGC GCC^GGC NgoMIV 4043 GCCGGC G^CCGGC PaeR7I 5087 CTCGAG C^TCGAG PflFI 5621 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1386 CCATCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 4318 AACGTT AA^CGTT RsrII 3547 CGGTCCG CG^G[AT]CCG SapI 2630 GAAGAGC GCTCTTC(1/4) SexAI 1105 ACCTGGT A^CC[AT]GGT SspBI 4832 TGTACA T^GTACA Tth111I 5621 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1386 CCATCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1545 TCTAGA T^CTAGA XhoI 5087 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 2420 GGTACC G^GTACC 3173 GGTACC AflIII 303 ACACGT A^C[GA][CT]GT 3309 ACATGT AhdI 3786 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3925 GACCTAGGGTC AseI 1370 ATTAAT AT^TAAT 5231 ATTAAT AsnI 1370 ATTAAT AT^TAAT 5231 ATTAAT Asp718I 2420 GGTACC G^GTACC 3173 GGTACC AspEI 3786 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3925 GACCTAGGGTC AvrII 3927 CCTAGG C^CTAGG 5497 CCTAGG BbvCI 764 GCTGAGG CCTCAGC(-5/-2) 1144 GCTGAGG BglII 1930 AGATCT A^GATCT 4643 AGATCT BlnI 3927 CCTAGG C^CTAGG 5497 CCTAGG BsaI 1233 GAGACC GGTCTC(1/5) 5619 GAGACC BspMI 4452 ACCTGC ACCTGC(4/8) 4488 ACCTGC BsrDI 3880 CATTGC GCAATG(2/0) 4970 CATTGC BstAPI 1176 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4222 GCAAAGCATGC BstXI 1073 CCAGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3465 CCAAGATGCTGG Eam1105I 3786 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3925 GACCTAGGGTC HgaI 1173 GACGC GACGC(5/10) 3855 GCGTC KpnI 2420 GGTACC GGTAC^C 3173 GGTACC MfeI 4148 CAATTG C^AATTG 5042 CAATTG MunI 4148 CAATTG C^AATTG 5042 CAATTG NcoI 4020 CCATGG C^CATGG 5051 CCATGG NdeI 1852 CATATG CA^TATG 4739 CATATG PshAI 4593 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4737 GACATATGTC PshBI 1370 ATTAAT AT^TAAT 5231 ATTAAT PstI 2734 CTGCAG CTGCA^G 3967 CTGCAG XcmI 1633 CCAGTGAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5081 CCACAGCTCGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BsrBI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmaI SmlI SphI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI