##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AflII       
AgeI        Aor51HI     ApaI        AscI        Asp700I     
AviII       BamHI       BbeI        BbrPI       BcgI        
BclI        BfrI        BglI        BsaAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BssHII      Bst1107I    BstBI       BstUI       
BstZ17I     ClaI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KspI        MamI        MluI        
MroI        MvnI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PvuI        SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AclI         4318  AACGTT               AA^CGTT
AcyI         3854  GGCGTC               G[GA]^CG[CT]C
Alw44I       5538  GTGCAC               G^TGCAC
ApaLI        5538  GTGCAC               G^TGCAC
AspI         5621  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        4248  GGATAC               GTATCC(6/5)
BlpI          230  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      230  GCTTAGC              GC^T[ACGT]AGC
BsaHI        3854  GGCGTC               G[GA]^CG[CT]C
BsmBI        1525  GAGACG               CGTCTC(1/5)
Bsp1407I     4832  TGTACA               T^GTACA
BspLU11I     3309  ACATGT               A^CATGT
BsrFI        4043  GCCGGC               [GA]^CCGG[CT]
BsrGI        4832  TGTACA               T^GTACA
BssSI        4359  CACGAG               CACGAG(-5/-1)
BstEII       2517  GGTCACC              G^GT[ACGT]ACC
BstPI        2517  GGTCACC              G^GT[ACGT]ACC
CelII         230  GCTTAGC              GC^T[ACGT]AGC
Cfr10I       4043  GCCGGC               [GA]^CCGG[CT]
CpoI         3547  CGGTCCG              CG^G[AT]CCG
EcoO65I      2517  GGTCACC              G^GT[ACGT]ACC
HaeII        3114  GGCGCT               [GA]GCGC^[CT]
Hin1I        3854  GGCGTC               G[GA]^CG[CT]C
HincII       3922  GTTGAC               GT[CT]^[GA]AC
HindII       3922  GTTGAC               GT[CT]^[GA]AC
NaeI         4043  GCCGGC               GCC^GGC
NgoMIV       4043  GCCGGC               G^CCGGC
PaeR7I       5087  CTCGAG               C^TCGAG
PflFI        5621  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1386  CCATCCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     4318  AACGTT               AA^CGTT
RsrII        3547  CGGTCCG              CG^G[AT]CCG
SapI         2630  GAAGAGC              GCTCTTC(1/4)
SexAI        1105  ACCTGGT              A^CC[AT]GGT
SspBI        4832  TGTACA               T^GTACA
Tth111I      5621  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1386  CCATCCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1545  TCTAGA               T^CTAGA
XhoI         5087  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I       2420  GGTACC               G^GTACC
             3173  GGTACC              
AflIII        303  ACACGT               A^C[GA][CT]GT
             3309  ACATGT              
AhdI         3786  GACAGACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3925  GACCTAGGGTC         
AseI         1370  ATTAAT               AT^TAAT
             5231  ATTAAT              
AsnI         1370  ATTAAT               AT^TAAT
             5231  ATTAAT              
Asp718I      2420  GGTACC               G^GTACC
             3173  GGTACC              
AspEI        3786  GACAGACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3925  GACCTAGGGTC         
AvrII        3927  CCTAGG               C^CTAGG
             5497  CCTAGG              
BbvCI         764  GCTGAGG              CCTCAGC(-5/-2)
             1144  GCTGAGG             
BglII        1930  AGATCT               A^GATCT
             4643  AGATCT              
BlnI         3927  CCTAGG               C^CTAGG
             5497  CCTAGG              
BsaI         1233  GAGACC               GGTCTC(1/5)
             5619  GAGACC              
BspMI        4452  ACCTGC               ACCTGC(4/8)
             4488  ACCTGC              
BsrDI        3880  CATTGC               GCAATG(2/0)
             4970  CATTGC              
BstAPI       1176  GCAGAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4222  GCAAAGCATGC         
BstXI        1073  CCAGCATTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3465  CCAAGATGCTGG        
Eam1105I     3786  GACAGACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3925  GACCTAGGGTC         
HgaI         1173  GACGC                GACGC(5/10)
             3855  GCGTC               
KpnI         2420  GGTACC               GGTAC^C
             3173  GGTACC              
MfeI         4148  CAATTG               C^AATTG
             5042  CAATTG              
MunI         4148  CAATTG               C^AATTG
             5042  CAATTG              
NcoI         4020  CCATGG               C^CATGG
             5051  CCATGG              
NdeI         1852  CATATG               CA^TATG
             4739  CATATG              
PshAI        4593  GACAAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4737  GACATATGTC          
PshBI        1370  ATTAAT               AT^TAAT
             5231  ATTAAT              
PstI         2734  CTGCAG               CTGCA^G
             3967  CTGCAG              
XcmI         1633  CCAGTGAACTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5081  CCACAGCTCGAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PvuII       
RcaI        RsaI        SacI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmaI        
SmlI        SphI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmaCI       
XmaI