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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BbeI BfrI BglII BlnI BsiWI
BsmBI Bsp1407I BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FseI FspI KasI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII ScaI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 5122 GACGTC GACGT^C
Acc65I 2072 GGTACC G^GTACC
Aor51HI 3851 AGCGCT AGC^GCT
Asp700I 2532 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2072 GGTACC G^GTACC
BamHI 266 GGATCC G^GATCC
BbrPI 3265 CACGTG CAC^GTG
BsaAI 3265 CACGTG [CT]AC^GT[GA]
BsaBI 2460 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4592 CGACCG CG[GA][CT]^CG
BspDI 2152 ATCGAT AT^CGAT
BspHI 146 TCATGA T^CATGA
BsrFI 5324 ACCGGC [GA]^CCGG[CT]
BssSI 2077 CACGAG CACGAG(-5/-1)
Cfr10I 5324 ACCGGC [GA]^CCGG[CT]
ClaI 2152 ATCGAT AT^CGAT
DrdI 1350 GACAGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3851 AGCGCT AGC^GCT
EcoRI 4924 GAATTC G^AATTC
HaeII 3851 AGCGCT [GA]GCGC^[CT]
HpaI 7047 GTTAAC GTT^AAC
KpnI 2072 GGTACC GGTAC^C
KspI 3623 CCGCGG CCGC^GG
MamI 2460 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PmaCI 3265 CACGTG CAC^GTG
PmlI 3265 CACGTG CAC^GTG
RcaI 146 TCATGA T^CATGA
SacII 3623 CCGCGG CCGC^GG
SalI 4590 GTCGAC G^TCGAC
SapI 6273 GAAGAGC GCTCTTC(1/4)
SbfI 1672 CCTGCAGG CCTGCA^GG
SphI 282 GCATGC GCATG^C
Sse8387I 1672 CCTGCAGG CCTGCA^GG
XmnI 2532 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1058 GTAGAC GT^[AC][GT]AC
4590 GTCGAC
AflIII 3695 ACATGT A^C[GA][CT]GT
5780 ACATGT
Alw44I 1498 GTGCAC G^TGCAC
5800 GTGCAC
AocI 3487 CCTGAGG CC^T[ACGT]AGG
4584 CCTCAGG
ApaLI 1498 GTGCAC G^TGCAC
5800 GTGCAC
AspI 132 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5251 GACCAGGTC
BcgI 4230 CGACATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2022 GCAGCCACCTCG
BciVI 5762 GTATCC GTATCC(6/5)
6437 GTATCC
BclI 572 TGATCA T^GATCA
7231 TGATCA
BsmI 885 GCATTC GAATGC(1/-1)
4705 GCATTC
BspLU11I 3695 ACATGT A^CATGT
5780 ACATGT
BsrBI 1298 CCGCTC CCGCTC(-3/-3)
5459 GAGCGG
BsrDI 4236 CATTGC GCAATG(2/0)
6757 CATTGC
BstAPI 2178 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4511 GCAGGAGCTGC
Bsu36I 3487 CCTGAGG CC^T[ACGT]AGG
4584 CCTCAGG
DraIII 4689 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
6347 CACACGGTG
Eco81I 3487 CCTGAGG CC^T[ACGT]AGG
4584 CCTCAGG
FbaI 572 TGATCA T^GATCA
7231 TGATCA
PflFI 132 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5251 GACCAGGTC
Tth111I 132 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5251 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfiI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI