##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Aor51HI AscI Asp718I AspI AvaI
AviII AvrII BanI BbeI BcgI
BciVI BclI BcnI BglI BlnI
BsaHI BsaI BsgI BsiEI BsiWI
BsoBI BspDI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoRI FauI FbaI FseI
FspI Hin1I KasI KpnI KspI
MluI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1409 GTCGAC GT^[AC][GT]AC
AccII 4341 CGCG CG^CG
AflII 3594 CTTAAG C^TTAAG
AhdI 1033 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2637 CCTGAGG CC^T[ACGT]AGG
ApaI 3747 GGGCCC GGGCC^C
AspEI 1033 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3261 TGGCCA TGG^CCA
BamHI 2999 GGATCC G^GATCC
BbrPI 4118 CACGTG CAC^GTG
BfrI 3594 CTTAAG C^TTAAG
BlpI 481 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 481 GCTGAGC GC^T[ACGT]AGC
BsaBI 2913 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4100 GAGACG CGTCTC(1/5)
Bsp1407I 2810 TGTACA T^GTACA
BsrGI 2810 TGTACA T^GTACA
BstEII 2829 GGTTACC G^GT[ACGT]ACC
BstPI 2829 GGTTACC G^GT[ACGT]ACC
BstUI 4341 CGCG CG^CG
Bsu36I 2637 CCTGAGG CC^T[ACGT]AGG
CelII 481 GCTGAGC GC^T[ACGT]AGC
EaeI 3261 TGGCCA [CT]^GGCC[GA]
Eam1105I 1033 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2637 CCTGAGG CC^T[ACGT]AGG
EcoNI 4235 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2829 GGTTACC G^GT[ACGT]ACC
HaeII 4000 AGCGCC [GA]GCGC^[CT]
HgaI 4296 GACGC GACGC(5/10)
HindIII 872 AAGCTT A^AGCTT
HpaI 3809 GTTAAC GTT^AAC
MamI 2913 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3261 TGGCCA TGG^CCA
MscI 3261 TGGCCA TGG^CCA
MvnI 4341 CGCG CG^CG
NruI 4340 TCGCGA TCG^CGA
PflMI 3003 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4118 CACGTG CAC^GTG
PmlI 4118 CACGTG CAC^GTG
PshAI 5128 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1409 GTCGAC G^TCGAC
SapI 790 GAAGAGC GCTCTTC(1/4)
SexAI 1809 ACCTGGT A^CC[AT]GGT
SpeI 2973 ACTAGT A^CTAGT
SphI 2581 GCATGC GCATG^C
SspBI 2810 TGTACA T^GTACA
SwaI 5268 ATTTAAAT ATTT^AAAT
Van91I 3003 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 927 TCCGGA T^CCGGA
2798 TCCGGA
Alw44I 1141 GTGCAC G^TGCAC
3693 GTGCAC
ApaLI 1141 GTGCAC G^TGCAC
3693 GTGCAC
BbsI 634 GAAGAC GAAGAC(2/6)
676 GAAGAC
BbvCI 2403 GCTGAGG CCTCAGC(-5/-2)
3737 GCTGAGG
BpuAI 634 GAAGAC GAAGAC(2/6)
676 GAAGAC
BsaAI 171 TACGTG [CT]AC^GT[GA]
4118 CACGTG
BseAI 927 TCCGGA T^CCGGA
2798 TCCGGA
BsmI 2721 GAATGC GAATGC(1/-1)
1622 GCATTC
BspEI 927 TCCGGA T^CCGGA
2798 TCCGGA
BspMI 332 ACCTGC ACCTGC(4/8)
4204 GCAGGT
BstAPI 2679 GCAAGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4062 GCACCTAATGC
EcoT14I 1444 CCAAGG C^C[AT][AT]GG
4414 CCAAGG
EcoT22I 2686 ATGCAT ATGCA^T
2723 ATGCAT
MfeI 355 CAATTG C^AATTG
4174 CAATTG
MroI 927 TCCGGA T^CCGGA
2798 TCCGGA
MunI 355 CAATTG C^AATTG
4174 CAATTG
NsiI 2686 ATGCAT ATGCA^T
2723 ATGCAT
PpuMI 4134 AGGTCCT [GA]G^G[AT]CC[CT]
5088 GGGTCCT
SacI 1591 GAGCTC GAGCT^C
1642 GAGCTC
StyI 1444 CCAAGG C^C[AT][AT]GG
4414 CCAAGG
XbaI 2046 TCTAGA T^CTAGA
4213 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbvI
BfaI BglII BmyI BpmI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoRV Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StuI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI