##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AcyI AgeI AhdI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BciVI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsiWI
BsmBI BsoBI BspDI BspHI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I EcoO65I
FauI FseI FspI Hin1I HpaI
KasI KpnI KspI MluI NarI
NotI NruI NspV PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 3352 AACGTT AA^CGTT
Alw44I 3238 GTGCAC G^TGCAC
AocI 2481 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2104 AGCGCT AGC^GCT
ApaLI 3238 GTGCAC G^TGCAC
BamHI 5345 GGATCC G^GATCC
BanI 2678 GGCACC G^G[CT][GA]CC
BanII 1853 GGGCTC G[GA]GC[CT]^C
BcgI 3358 GCATCTTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3583 TGATCA T^GATCA
BsaBI 100 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3113 CGATCG CG[GA][CT]^CG
BsrBI 498 GAGCGG CCGCTC(-3/-3)
BstAPI 2995 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 216 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2481 CCTGAGG CC^T[ACGT]AGG
DrdI 4221 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 5075 CCATGG C^C[GA][CT]GG
Eco47III 2104 AGCGCT AGC^GCT
Eco81I 2481 CCTGAGG CC^T[ACGT]AGG
EcoRI 2487 GAATTC G^AATTC
FbaI 3583 TGATCA T^GATCA
HincII 1524 GTTGAC GT[CT]^[GA]AC
HindII 1524 GTTGAC GT[CT]^[GA]AC
MamI 100 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 758 CAATTG C^AATTG
MunI 758 CAATTG C^AATTG
NaeI 3396 GCCGGC GCC^GGC
NcoI 5075 CCATGG C^CATGG
NgoMIV 3396 GCCGGC G^CCGGC
NheI 4845 GCTAGC G^CTAGC
PacI 5247 TTAATTAA TTAAT^TAA
PflMI 3005 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3352 AACGTT AA^CGTT
PvuI 3113 CGATCG CGAT^CG
SwaI 101 ATTTAAAT ATTT^AAAT
Van91I 3005 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 232 CGCG CG^CG
234 CGCG
AccIII 921 TCCGGA T^CCGGA
2852 TCCGGA
AflII 2135 CTTAAG C^TTAAG
4238 CTTAAG
AflIII 4439 ACATGT A^C[GA][CT]GT
5146 ACATGT
AlwNI 1096 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1468 CAGACCCTG
AseI 3552 ATTAAT AT^TAAT
4066 ATTAAT
AsnI 3552 ATTAAT AT^TAAT
4066 ATTAAT
AspHI 1935 GTGCTC G[AT]GC[AT]^C
3238 GTGCAC
BalI 1736 TGGCCA TGG^CCA
1816 TGGCCA
BcnI 188 CCGGG CC^[GC]GG
283 CCCGG
BfrI 2135 CTTAAG C^TTAAG
4238 CTTAAG
BglII 852 AGATCT A^GATCT
1488 AGATCT
BsaWI 921 TCCGGA [AT]^CCGG[AT]
2852 TCCGGA
BseAI 921 TCCGGA T^CCGGA
2852 TCCGGA
BseRI 1648 CTCCTC GAGGAG(10/8)
765 GAGGAG
BsiHKAI 1935 GTGCTC G[AT]GC[AT]^C
3238 GTGCAC
Bsp1407I 3298 TGTACA T^GTACA
5149 TGTACA
BspEI 921 TCCGGA T^CCGGA
2852 TCCGGA
BspLU11I 4439 ACATGT A^CATGT
5146 ACATGT
BsrFI 2084 GCCGGT [GA]^CCGG[CT]
3396 GCCGGC
BsrGI 3298 TGTACA T^GTACA
5149 TGTACA
BstUI 232 CGCG CG^CG
234 CGCG
Cfr10I 2084 GCCGGT [GA]^CCGG[CT]
3396 GCCGGC
DraII 893 AGGACCC [GA]G^G[ACGT]CC[CT]
1097 AGGTCCT
EcoNI 60 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2018 CCTGAAGAAGG
EcoO109I 893 AGGACCC [GA]G^G[ACGT]CC[CT]
1097 AGGTCCT
EcoRV 1347 GATATC GAT^ATC
1607 GATATC
EcoT22I 3259 ATGCAT ATGCA^T
4897 ATGCAT
HaeII 2027 GGCGCT [GA]GCGC^[CT]
2104 AGCGCT
HgaI 1367 GACGC GACGC(5/10)
235 GCGTC
HindIII 1223 AAGCTT A^AGCTT
3563 AAGCTT
MluNI 1736 TGGCCA TGG^CCA
1816 TGGCCA
MroI 921 TCCGGA T^CCGGA
2852 TCCGGA
MscI 1736 TGGCCA TGG^CCA
1816 TGGCCA
MvnI 232 CGCG CG^CG
234 CGCG
NciI 188 CCGGG CC^[GC]GG
283 CCCGG
NsiI 3259 ATGCAT ATGCA^T
4897 ATGCAT
PpuMI 893 AGGACCC [GA]G^G[AT]CC[CT]
1097 AGGTCCT
PshBI 3552 ATTAAT AT^TAAT
4066 ATTAAT
PvuII 846 CAGCTG CAG^CTG
2863 CAGCTG
ScaI 3178 AGTACT AGT^ACT
4259 AGTACT
SspBI 3298 TGTACA T^GTACA
5149 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI