################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AccIII AgeI AhdI Aor51HI ApaI AscI Asp700I Asp718I AspEI AviII BbeI BcgI BglI BglII BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FauI FseI FspI HaeII HgaI HindIII KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PpuMI PshAI PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 1612 GACGTC GACGT^C AccII 940 CGCG CG^CG AclI 3913 AACGTT AA^CGTT AcyI 1612 GACGTC G[GA]^CG[CT]C AflII 4980 CTTAAG C^TTAAG Alw44I 1520 GTGCAC G^TGCAC ApaLI 1520 GTGCAC G^TGCAC AseI 4308 ATTAAT AT^TAAT AsnI 4308 ATTAAT AT^TAAT AvrII 3373 CCTAGG C^CTAGG BamHI 5882 GGATCC G^GATCC BbrPI 1501 CACGTG CAC^GTG BfrI 4980 CTTAAG C^TTAAG BlnI 3373 CCTAGG C^CTAGG BsaAI 1501 CACGTG [CT]AC^GT[GA] BsaHI 1612 GACGTC G[GA]^CG[CT]C Bsp1407I 4513 TGTACA T^GTACA BspMI 1441 GCAGGT ACCTGC(4/8) BsrGI 4513 TGTACA T^GTACA BssSI 1414 CTCGTG CACGAG(-5/-1) BstUI 940 CGCG CG^CG DraIII 2163 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 3362 GAATTC G^AATTC Hin1I 1612 GACGTC G[GA]^CG[CT]C HpaI 5639 GTTAAC GTT^AAC MfeI 1154 CAATTG C^AATTG MunI 1154 CAATTG C^AATTG MvnI 940 CGCG CG^CG NdeI 2369 CATATG CA^TATG NheI 3420 GCTAGC G^CTAGC PmaCI 1501 CACGTG CAC^GTG PmeI 5628 GTTTAAAC GTTT^AAAC PmlI 1501 CACGTG CAC^GTG PshBI 4308 ATTAAT AT^TAAT Psp1406I 3913 AACGTT AA^CGTT SalI 2155 GTCGAC G^TCGAC SphI 2103 GCATGC GCATG^C SspBI 4513 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 3002 ACATGT A^C[GA][CT]GT 5332 ACATGT AlwNI 268 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 1403 CAGTGGCTG AvaI 4025 CCCGGG C^[CT]CG[GA]G 4223 CCCGGG AvaII 308 GGACC G^G[AT]CC 1311 GGACC BalI 869 TGGCCA TGG^CCA 1890 TGGCCA BanI 11 GGTGCC G^G[CT][GA]CC 1578 GGTGCC BclI 1752 TGATCA T^GATCA 4958 TGATCA BsmI 227 GAATGC GAATGC(1/-1) 1030 GAATGC BsoBI 4025 CCCGGG C^[CT]CG[GA]G 4223 CCCGGG BspLU11I 3002 ACATGT A^CATGT 5332 ACATGT BsrDI 4260 CATTGC GCAATG(2/0) 106 GCAATG BstAPI 1402 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3518 GCAGGAAATGC EaeI 869 TGGCCA [CT]^GGCC[GA] 1890 TGGCCA EcoNI 615 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5617 CCTCAAATAGG EcoRV 1904 GATATC GAT^ATC 4872 GATATC FbaI 1752 TGATCA T^GATCA 4958 TGATCA MluNI 869 TGGCCA TGG^CCA 1890 TGGCCA MscI 869 TGGCCA TGG^CCA 1890 TGGCCA MspA1I 1944 CAGCTG C[AC]G^C[GT]G 3118 CAGCTG PflMI 1975 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5254 CCACATGCTGG PvuII 1944 CAGCTG CAG^CTG 3118 CAGCTG SacI 187 GAGCTC GAGCT^C 2553 GAGCTC SmaI 4025 CCCGGG CCC^GGG 4223 CCCGGG StuI 2097 AGGCCT AGG^CCT 4583 AGGCCT TaqI 1616 TCGA T^CGA 2156 TCGA TthHB8I 1616 TCGA T^CGA 2156 TCGA Van91I 1975 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5254 CCACATGCTGG XbaI 954 TCTAGA T^CTAGA 2403 TCTAGA XmaCI 4025 CCCGGG C^CCGGG 4223 CCCGGG XmaI 4025 CCCGGG C^CCGGG 4223 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AocI ApoI AspHI AspI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflFI PleI PstI RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I XcmI XhoII