##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       AclI        AgeI        Aor51HI     AscI        
AseI        AsnI        AvrII       BbeI        BlnI        
BsiWI       BspDI       BspLU11I    BsrFI       BssHII      
BstBI       Cfr10I      ClaI        CpoI        DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FseI        HpaI        KasI        MfeI        
MluI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AhdI         2565  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2828  GTGCAC               G^TGCAC
AocI         2546  CCTCAGG              CC^T[ACGT]AGG
ApaI         1984  GGGCCC               GGGCC^C
ApaLI        2828  GTGCAC               G^TGCAC
AspEI        2565  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1929  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI          78  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2620  CGATCCTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI        2765  CGACCG               CG[GA][CT]^CG
BsmI         3958  GAATGC               GAATGC(1/-1)
Bst1107I     4450  GTATAC               GTA^TAC
BstZ17I      4450  GTATAC               GTA^TAC
Bsu36I       2546  CCTCAGG              CC^T[ACGT]AGG
Eam1105I     2565  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2546  CCTCAGG              CC^T[ACGT]AGG
HaeII          75  GGCGCT               [GA]GCGC^[CT]
HindIII      4436  AAGCTT               A^AGCTT
KspI         1519  CCGCGG               CCGC^GG
NcoI         2002  CCATGG               C^CATGG
PaeR7I       4935  CTCGAG               C^TCGAG
PflFI        1929  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI          357  GAGCTC               GAGCT^C
SacII        1519  CCGCGG               CCGC^GG
SmaI         2152  CCCGGG               CCC^GGG
SphI         1970  GCATGC               GCATG^C
Tth111I      1929  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         4935  CTCGAG               C^TCGAG
XmaCI        2152  CCCGGG               C^CCGGG
XmaI         2152  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       1191  GGTACC               G^GTACC
             1501  GGTACC              
AccI          402  GTCTAC               GT^[AC][GT]AC
             4450  GTATAC              
AccIII        435  TCCGGA               T^CCGGA
             2472  TCCGGA              
AcyI         1961  GACGCC               G[GA]^CG[CT]C
             4615  GGCGTC              
AflII        4439  CTTAAG               C^TTAAG
             4544  CTTAAG              
AflIII       1767  ACGTGT               A^C[GA][CT]GT
             4454  ACGTGT              
Asp700I      2358  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2417  GAATAAGTTC          
Asp718I      1191  GGTACC               G^GTACC
             1501  GGTACC              
AviII         637  TGCGCA               TGC^GCA
             3240  TGCGCA              
BamHI         895  GGATCC               G^GATCC
             1592  GGATCC              
BbrPI         972  CACGTG               CAC^GTG
             1766  CACGTG              
BbsI         2686  GAAGAC               GAAGAC(2/6)
             3696  GAAGAC              
BclI         2432  TGATCA               T^GATCA
             3163  TGATCA              
BfrI         4439  CTTAAG               C^TTAAG
             4544  CTTAAG              
BglII        2407  AGATCT               A^GATCT
             4575  AGATCT              
BlpI         1166  GCTCAGC              GC^T[ACGT]AGC
             1352  GCTGAGC             
Bpu1102I     1166  GCTCAGC              GC^T[ACGT]AGC
             1352  GCTGAGC             
BpuAI        2686  GAAGAC               GAAGAC(2/6)
             3696  GAAGAC              
BsaBI         239  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2361  GATTTTCATC          
BsaHI        1961  GACGCC               G[GA]^CG[CT]C
             4615  GGCGTC              
BsaI         2135  GAGACC               GGTCTC(1/5)
             3212  GAGACC              
BseAI         435  TCCGGA               T^CCGGA
             2472  TCCGGA              
BspEI         435  TCCGGA               T^CCGGA
             2472  TCCGGA              
BspHI        4340  TCATGA               T^CATGA
             4370  TCATGA              
BssSI        2721  CACGAG               CACGAG(-5/-1)
             1016  CTCGTG              
BstAPI       1699  GCAGGTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2821  GCACAGTGTGC         
BstEII        885  GGTGACC              G^GT[ACGT]ACC
             1236  GGTGACC             
BstPI         885  GGTGACC              G^GT[ACGT]ACC
             1236  GGTGACC             
CelII        1166  GCTCAGC              GC^T[ACGT]AGC
             1352  GCTGAGC             
DraI         3103  TTTAAA               TTT^AAA
             5421  TTTAAA              
DraIII        136  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2822  CACAGTGTG           
EcoO65I       885  GGTGACC              G^GT[ACGT]ACC
             1236  GGTGACC             
FbaI         2432  TGATCA               T^GATCA
             3163  TGATCA              
FspI          637  TGCGCA               TGC^GCA
             3240  TGCGCA              
Hin1I        1961  GACGCC               G[GA]^CG[CT]C
             4615  GGCGTC              
HincII        546  GTCAAC               GT[CT]^[GA]AC
             3676  GTCAAC              
HindII        546  GTCAAC               GT[CT]^[GA]AC
             3676  GTCAAC              
KpnI         1191  GGTACC               GGTAC^C
             1501  GGTACC              
MamI          239  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2361  GATTTTCATC          
MroI          435  TCCGGA               T^CCGGA
             2472  TCCGGA              
PflMI        1251  CCAGCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1788  CCACAGTTTGG         
PmaCI         972  CACGTG               CAC^GTG
             1766  CACGTG              
PmlI          972  CACGTG               CAC^GTG
             1766  CACGTG              
RcaI         4340  TCATGA               T^CATGA
             4370  TCATGA              
SexAI        1822  ACCAGGT              A^CC[AT]GGT
             4536  ACCTGGT             
Van91I       1251  CCAGCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1788  CCACAGTTTGG         
XbaI         4018  TCTAGA               T^CTAGA
             4555  TCTAGA              
XmnI         2358  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2417  GAATAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BciVI       BcnI        BfaI        BglI        BmyI        
BpmI        BsaAI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII