################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AclI AgeI Aor51HI AscI AseI AsnI AvrII BbeI BlnI BsiWI BspDI BspLU11I BsrFI BssHII BstBI Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI HpaI KasI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AhdI 2565 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2828 GTGCAC G^TGCAC AocI 2546 CCTCAGG CC^T[ACGT]AGG ApaI 1984 GGGCCC GGGCC^C ApaLI 2828 GTGCAC G^TGCAC AspEI 2565 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1929 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 78 GCTGAGG CCTCAGC(-5/-2) BcgI 2620 CGATCCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsiEI 2765 CGACCG CG[GA][CT]^CG BsmI 3958 GAATGC GAATGC(1/-1) Bst1107I 4450 GTATAC GTA^TAC BstZ17I 4450 GTATAC GTA^TAC Bsu36I 2546 CCTCAGG CC^T[ACGT]AGG Eam1105I 2565 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2546 CCTCAGG CC^T[ACGT]AGG HaeII 75 GGCGCT [GA]GCGC^[CT] HindIII 4436 AAGCTT A^AGCTT KspI 1519 CCGCGG CCGC^GG NcoI 2002 CCATGG C^CATGG PaeR7I 4935 CTCGAG C^TCGAG PflFI 1929 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 357 GAGCTC GAGCT^C SacII 1519 CCGCGG CCGC^GG SmaI 2152 CCCGGG CCC^GGG SphI 1970 GCATGC GCATG^C Tth111I 1929 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4935 CTCGAG C^TCGAG XmaCI 2152 CCCGGG C^CCGGG XmaI 2152 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 1191 GGTACC G^GTACC 1501 GGTACC AccI 402 GTCTAC GT^[AC][GT]AC 4450 GTATAC AccIII 435 TCCGGA T^CCGGA 2472 TCCGGA AcyI 1961 GACGCC G[GA]^CG[CT]C 4615 GGCGTC AflII 4439 CTTAAG C^TTAAG 4544 CTTAAG AflIII 1767 ACGTGT A^C[GA][CT]GT 4454 ACGTGT Asp700I 2358 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2417 GAATAAGTTC Asp718I 1191 GGTACC G^GTACC 1501 GGTACC AviII 637 TGCGCA TGC^GCA 3240 TGCGCA BamHI 895 GGATCC G^GATCC 1592 GGATCC BbrPI 972 CACGTG CAC^GTG 1766 CACGTG BbsI 2686 GAAGAC GAAGAC(2/6) 3696 GAAGAC BclI 2432 TGATCA T^GATCA 3163 TGATCA BfrI 4439 CTTAAG C^TTAAG 4544 CTTAAG BglII 2407 AGATCT A^GATCT 4575 AGATCT BlpI 1166 GCTCAGC GC^T[ACGT]AGC 1352 GCTGAGC Bpu1102I 1166 GCTCAGC GC^T[ACGT]AGC 1352 GCTGAGC BpuAI 2686 GAAGAC GAAGAC(2/6) 3696 GAAGAC BsaBI 239 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2361 GATTTTCATC BsaHI 1961 GACGCC G[GA]^CG[CT]C 4615 GGCGTC BsaI 2135 GAGACC GGTCTC(1/5) 3212 GAGACC BseAI 435 TCCGGA T^CCGGA 2472 TCCGGA BspEI 435 TCCGGA T^CCGGA 2472 TCCGGA BspHI 4340 TCATGA T^CATGA 4370 TCATGA BssSI 2721 CACGAG CACGAG(-5/-1) 1016 CTCGTG BstAPI 1699 GCAGGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2821 GCACAGTGTGC BstEII 885 GGTGACC G^GT[ACGT]ACC 1236 GGTGACC BstPI 885 GGTGACC G^GT[ACGT]ACC 1236 GGTGACC CelII 1166 GCTCAGC GC^T[ACGT]AGC 1352 GCTGAGC DraI 3103 TTTAAA TTT^AAA 5421 TTTAAA DraIII 136 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2822 CACAGTGTG EcoO65I 885 GGTGACC G^GT[ACGT]ACC 1236 GGTGACC FbaI 2432 TGATCA T^GATCA 3163 TGATCA FspI 637 TGCGCA TGC^GCA 3240 TGCGCA Hin1I 1961 GACGCC G[GA]^CG[CT]C 4615 GGCGTC HincII 546 GTCAAC GT[CT]^[GA]AC 3676 GTCAAC HindII 546 GTCAAC GT[CT]^[GA]AC 3676 GTCAAC KpnI 1191 GGTACC GGTAC^C 1501 GGTACC MamI 239 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2361 GATTTTCATC MroI 435 TCCGGA T^CCGGA 2472 TCCGGA PflMI 1251 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1788 CCACAGTTTGG PmaCI 972 CACGTG CAC^GTG 1766 CACGTG PmlI 972 CACGTG CAC^GTG 1766 CACGTG RcaI 4340 TCATGA T^CATGA 4370 TCATGA SexAI 1822 ACCAGGT A^CC[AT]GGT 4536 ACCTGGT Van91I 1251 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1788 CCACAGTTTGG XbaI 4018 TCTAGA T^CTAGA 4555 TCTAGA XmnI 2358 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2417 GAATAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BciVI BcnI BfaI BglI BmyI BpmI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII